################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AfaI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MaeII MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 39 GTCTAC GT^[AC][GT]AC AflII 187 CTTAAG C^TTAAG AlwI 148 GATCC GGATC(4/5) AocI 107 CCTTAGG CC^T[ACGT]AGG AvrII 775 CCTAGG C^CTAGG BalI 644 TGGCCA TGG^CCA BbsI 329 GTCTTC GAAGAC(2/6) BfrI 187 CTTAAG C^TTAAG BglII 96 AGATCT A^GATCT BlnI 775 CCTAGG C^CTAGG BpuAI 329 GTCTTC GAAGAC(2/6) BsaBI 9 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 677 GAATGC GAATGC(1/-1) BstAPI 496 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 225 CCATGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 96 AGATCT [GA]^GATC[CT] Bsu36I 107 CCTTAGG CC^T[ACGT]AGG Cac8I 747 GCCAGC GC[ACGT]^[ACGT]GC DraI 603 TTTAAA TTT^AAA DraIII 168 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 644 TGGCCA [CT]^GGCC[GA] EarI 611 GAAGAG CTCTTC(1/4) Eco81I 107 CCTTAGG CC^T[ACGT]AGG EcoRI 549 GAATTC G^AATTC EcoRV 785 GATATC GAT^ATC FauI 50 GCGGG CCCGC(4/6) HincII 471 GTTAAC GT[CT]^[GA]AC HindII 471 GTTAAC GT[CT]^[GA]AC HpaI 471 GTTAAC GTT^AAC HphI 339 TCACC GGTGA(8/7) Ksp632I 611 GAAGAG CTCTTC(1/4) MamI 9 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 96 AGATCT [GA]^GATC[CT] MluNI 644 TGGCCA TGG^CCA MscI 644 TGGCCA TGG^CCA MslI 118 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG SfaNI 456 GATGC GCATC(5/9) SmlI 187 CTTAAG C^T[CT][GA]AG SwaI 602 ATTTAAAT ATTT^AAAT TaqI 92 TCGA T^CGA TthHB8I 92 TCGA T^CGA XhoII 96 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AvaII 53 GGTCC G^G[AT]CC 633 GGACC BbvI 522 GCAGC GCAGC(8/12) 811 GCTGC BglI 765 GCCAAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 814 GCCATGGCGGC BpmI 735 CTCCAG CTGGAG(16/14) 752 CTCCAG BstF5I 830 CATCC GGATG(2/0) 8 GGATG Cfr13I 53 GGTCC G^G[ACGT]CC 633 GGACC DpnII 97 GATC ^GATC 148 GATC DsaI 32 CCATGG C^C[GA][CT]GG 815 CCATGG FokI 830 CATCC GGATG(9/13) 8 GGATG HaeIII 645 GGCC GG^CC 773 GGCC MboI 97 GATC ^GATC 148 GATC MnlI 485 CCTC CCTC(7/6) 803 GAGG NcoI 32 CCATGG C^CATGG 815 CCATGG NdeII 97 GATC ^GATC 148 GATC NlaIV 52 GGGTCC GG[ACGT]^[ACGT]CC 633 GGACCC PleI 366 GACTC GAGTC(4/5) 37 GAGTC Sau3AI 97 GATC ^GATC 148 GATC Sau96I 53 GGTCC G^G[ACGT]CC 633 GGACC TseI 522 GCAGC G^C[AT]GC 811 GCTGC TspRI 172 CAGTG CA[GC]TG[ACGT][ACGT]^ 300 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AcsI AluI ApoI BfaI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI DdeI EcoRII EcoT14I Fnu4HI HinfI ItaI MaeI MaeIII MboII MseI MvaI MwoI NlaIII ScrFI StyI TfiI Tru9I Tsp45I Tsp509I