Restriction map for
pFN21AE5346
(Circular) (Six-base) MAPSORT of: pFN21AE5346.seq Check: 4280 from: 1 to: 5560
pFN21AE5346 5560 bp
With 182 enzymes: SgfI *
January 30, 2014 17:17 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2045 2903 1937
Size: 108 858 4594
Fragments arranged by size:
4594 858 108
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2126 2399 3623 5007 5275 1632
Size: 494 273 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 494 273 268
AflII C'TTAA_G
Cuts at: 820 1017 2192 820
Size: 197 1175 4188
Fragments arranged by size:
4188 1175 197
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5560
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 3303 4875 3303
Size: 1572 3988
ApaLI G'TGCA_C
Cuts at: 3617 4773 3617
Size: 1156 4404
ApoI r'AATT_y
Cuts at: 1493 2225 2455 2554 2605 2869 3095 3247 5356
Size: 732 230 99 51 264 226 152 2109
Cuts at: 5356 1493
Size: 1697
Fragments arranged by size:
2109 1697 732 264 230 226 152 99
51
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 1870
Size: 73 942 4545
Fragments arranged by size:
4545 942 73
AvrII C'CTAG_G
Cuts at: 2780 2780
Size: 5560
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2881 4336 618
Size: 325 217 627 1094 1455 1842
Fragments arranged by size:
1842 1455 1094 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2879 1391
Size: 1488 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2327 928
Size: 971 428 4161
Fragments arranged by size:
4161 971 428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2776 2825 4171
Size: 107 122 71 631 605 1104 49 1346
Cuts at: 4171 136
Size: 1525
Fragments arranged by size:
1525 1346 1104 631 605 122 107 71
49
BglII A'GATC_T
Cuts at: 2101 5555 2101
Size: 3454 2106
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 3621 4777 1790
Size: 1831 1156 2573
Fragments arranged by size:
2573 1831 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5560
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3291 4249 442
Size: 416 53 83 399 24 1874 958 1753
Fragments arranged by size:
1874 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2725 2742 4220 1239
Size: 1486 17 1478 2579
Fragments arranged by size:
2579 1486 1478 17
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 2018 5554 2018
Size: 3536 2024
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3367 3749 4384 275
Size: 53 83 186 936 1834 382 635 1451
Fragments arranged by size:
1834 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3993 4391 4665 4812 5223 3993
Size: 398 274 147 411 4330
Fragments arranged by size:
4330 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5335
Fragments arranged by size:
5335 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5560
BseYI C'CCAG_C
Cuts at: 4763 5259 4763
Size: 496 5064
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4987
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3621 3706 4777 3621
Size: 85 1071 4404
Fragments arranged by size:
4404 1071 85
BsmI GAATG_Cn'
Cuts at: 2688 3071 3164 2688
Size: 383 93 5084
Fragments arranged by size:
5084 383 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2879 3621 3706 4777 1391
Size: 399 1089 742 85 1071 2174
Fragments arranged by size:
2174 1089 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5223 5223
Size: 5560
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2915 844
Size: 2071 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3447 1394
Size: 429 1624 3507
Fragments arranged by size:
3507 1624 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4055 4229 65
Size: 1183 2807 174 1396
Fragments arranged by size:
2807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4204 4391 1700
Size: 110 2394 187 2869
Fragments arranged by size:
2869 2394 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2507 794
Size: 1713 3847
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5560
BstXI CCAn_nnnn'nTGG
Cuts at: 2237 2237
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2101 2890 3257 3642 3659 5111 5197 5209
Size: 129 789 367 385 17 1452 86 12
Cuts at: 5209 5555 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 789 385 367 346 129 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2113 2113
Size: 5560
BtgI C'CryG_G
Cuts at: 513 1065 2037 2820 513
Size: 552 972 783 3253
Fragments arranged by size:
3253 972 783 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 3076 3870 3890 3076
Size: 794 20 4746
Fragments arranged by size:
4746 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2610 2864 2610
Size: 254 5306
DraIII CAC_nnn'GTG
Cuts at: 2178 2178
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2649 3007 3896
Size: 54 1425 50 275 18 819 358 889
Cuts at: 3896 5525 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 819 358 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2611 3490 1353
Size: 1258 879 3423
Fragments arranged by size:
3423 1258 879
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2026 4147 4521 4667 387
Size: 1064 138 437 2121 374 146 1280
Fragments arranged by size:
2121 1280 1064 437 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2126 2399 2725 2742 3623 4220 5007
Size: 393 494 273 326 17 881 597 787
Cuts at: 5007 5275 1239
Size: 268 1524
Fragments arranged by size:
1524 881 787 597 494 393 326 273
268 17
EcoRI G'AATT_C
Cuts at: 2554 2869 3247 2554
Size: 315 378 4867
Fragments arranged by size:
4867 378 315
EcoRV GAT'ATC
Cuts at: 2792 2792
Size: 5560
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2719 2719
Size: 5560
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 4707 1678
Size: 3029 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2093 2125 2199 2231 2279
Size: 32 135 32 521 32 74 32 48
Cuts at: 2279 2311 2776 2808 4201 4233 4275 4307 1373
Size: 32 465 32 1393 32 42 32 2626
Fragments arranged by size:
2626 1393 521 465 135 74 48 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2478 2904 3150 669
Size: 988 281 540 426 246 3079
Fragments arranged by size:
3079 988 540 426 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 2478 3150 2478
Size: 672 4888
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2246 4674 4858 1305
Size: 941 2428 184 2007
Fragments arranged by size:
2428 2007 941 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2651 10
Size: 54 1425 343 819 2919
Fragments arranged by size:
2919 1425 819 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2127 3518 3877 4036 518
Size: 648 961 1391 359 159 2042
Fragments arranged by size:
2042 1391 961 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2976 3653 4801 5046 1606
Size: 1370 677 1148 245 2120
Fragments arranged by size:
2120 1370 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2037 2820 513
Size: 552 972 783 3253
Fragments arranged by size:
3253 972 783 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2918 4463 1132
Size: 97 1689 1545 2229
Fragments arranged by size:
2229 1689 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2065 1348
Size: 717 4843
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5560
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2912 830
Size: 2082 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2908 4043 4724 4915 826
Size: 219 1863 1135 681 191 1471
Fragments arranged by size:
1863 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 3664 4565 4827 820
Size: 197 926 249 1472 901 262 1553
Fragments arranged by size:
1553 1472 926 901 262 249 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5560
StyI C'CwwG_G
Cuts at: 513 1065 2037 2588 2780 2820 2999 513
Size: 552 972 551 192 40 179 3074
Fragments arranged by size:
3074 972 552 551 192 179 40
SwaI ATTT'AAAT
Cuts at: 2610 2610
Size: 5560
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 3587 3604 3757 3942 4387 325
Size: 1255 519 1488 17 153 185 445 1498
Fragments arranged by size:
1498 1488 1255 519 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3806 5518 96
Size: 274 80 33 51 1443 1829 1712 138
Fragments arranged by size:
1829 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5520 5552 5520
Size: 32 5528
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 3689 3689
Size: 5560
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI
EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI