(Linear) (Six-base) MAPSORT of: KIEE5346.seq  Check: 3971  from: 1  to: 855



KIEE5346.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:56  ..

AccI GT'mk_AC

Cuts at:      0      40     855 
   Size:         40     815
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     394     855 
   Size:        121     273     461

  Fragments arranged by size: 

                 461     273     121
 
AflII C'TTAA_G

Cuts at:      0     187     855 
   Size:        187     668
 
ApoI r'AATT_y

Cuts at:      0     220     450     549     600     855 
   Size:        220     230      99      51     255

  Fragments arranged by size: 

                 255     230     220      99      51
 
AvrII C'CTAG_G

Cuts at:      0     775     855 
   Size:        775      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     322     855 
   Size:        322     533
 
BglI GCCn_nnn'nGGC

Cuts at:      0     771     820     855 
   Size:        771      49      35

  Fragments arranged by size: 

                 771      49      35
 
BglII A'GATC_T

Cuts at:      0      96     855 
   Size:         96     759
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     720     737     855 
   Size:        720      17     118

  Fragments arranged by size: 

                 720     118      17
 
BsaBI GATnn'nnATC

Cuts at:      0      13     855 
   Size:         13     842
 
BsmI GAATG_Cn'

Cuts at:      0     683     855 
   Size:        683     172
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     502     855 
   Size:        502     353
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     232     855 
   Size:        232     623
 
BstYI r'GATC_y

Cuts at:      0      96     855 
   Size:         96     759
 
Bsu36I CC'TnA_GG

Cuts at:      0     108     855 
   Size:        108     747
 
BtgI C'CryG_G

Cuts at:      0      32     815     855 
   Size:         32     783      40

  Fragments arranged by size: 

                 783      40      32
 
DraI TTT'AAA

Cuts at:      0     605     855 
   Size:        605     250
 
DraIII CAC_nnn'GTG

Cuts at:      0     173     855 
   Size:        173     682
 
EaeI y'GGCC_r

Cuts at:      0     644     855 
   Size:        644     211
 
EarI CTCTTCn'nnn_

Cuts at:      0     606     855 
   Size:        606     249
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      21     855 
   Size:         21     834
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     394     720     737     855 
   Size:        121     273     326      17     118

  Fragments arranged by size: 

                 326     273     121     118      17
 
EcoRI G'AATT_C

Cuts at:      0     549     855 
   Size:        549     306
 
EcoRV GAT'ATC

Cuts at:      0     787     855 
   Size:        787      68
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     714     855 
   Size:        714     141
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      88     120     194     226     274     306     771     803 
   Size:         88      32      74      32      48      32     465      32

Cuts at:    803     855 
   Size:         52

  Fragments arranged by size: 

                 465      88      74      52      48      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     473     855 
   Size:        473     382
 
HpaI GTT'AAC

Cuts at:      0     473     855 
   Size:        473     382
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     241     855 
   Size:        241     614
 
MscI TGG'CCA

Cuts at:      0     646     855 
   Size:        646     209
 
MslI CAynn'nnrTG

Cuts at:      0     122     855 
   Size:        122     733
 
NcoI C'CATG_G

Cuts at:      0      32     815     855 
   Size:         32     783      40

  Fragments arranged by size: 

                 783      40      32
 
PasI CC'CwG_GG

Cuts at:      0      60     855 
   Size:         60     795
 
SmlI C'TyrA_G

Cuts at:      0     187     855 
   Size:        187     668
 
StyI C'CwwG_G

Cuts at:      0      32     583     775     815     855 
   Size:         32     551     192      40      40

  Fragments arranged by size: 

                 551     192      40      40      32
 
SwaI ATTT'AAAT

Cuts at:      0     605     855 
   Size:        605     250
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      94     855 
   Size:         94     761
 

 Enzymes that do cut:

     AccI     AcuI    AflII     ApoI    AvrII     BbsI     BglI    BglII
     BpmI    BsaBI     BsmI   BstAPI    BstXI    BstYI   Bsu36I     BtgI
     DraI   DraIII     EaeI     EarI     EciI  Eco57MI    EcoRI    EcoRV
     FalI    Hin4I   HincII     HpaI     MmeI     MscI     MslI     NcoI
     PasI     SmlI     StyI     SwaI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I    BtgZI     BtsI     ClaI
    CspCI     DrdI     EagI  EcoICRI    EcoNI EcoO109I     FseI     FspI
    FspAI    HaeII  HindIII     KasI     KpnI     MfeI     MluI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI