Restriction map for
pFN21AE6492
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AhdI ApaI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BbeI BbrPI BbvCI
BcgI BfrI BlnI BlpI BpmI
Bpu1102I BsaBI BseAI BseRI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrFI BsrGI BssHII BstBI
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SbfI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp509I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 1777 GACGTC GACGT^C
AccII 480 CGCG CG^CG
AcyI 1777 GACGTC G[GA]^CG[CT]C
Alw44I 666 GTGCAC G^TGCAC
AocI 866 CCTCAGG CC^T[ACGT]AGG
ApaLI 666 GTGCAC G^TGCAC
BamHI 1112 GGATCC G^GATCC
BciVI 888 GTATCC GTATCC(6/5)
BclI 434 TGATCA T^GATCA
BfaI 530 CTAG C^TAG
BsaAI 1309 TACGTG [CT]AC^GT[GA]
BsaHI 1777 GACGTC G[GA]^CG[CT]C
BsaI 1098 GGTCTC GGTCTC(1/5)
BsmFI 714 GTCCC GGGAC(10/14)
BsmI 1361 GCATTC GAATGC(1/-1)
BspHI 1058 TCATGA T^CATGA
BspLU11I 290 ACATGT A^CATGT
BssSI 898 CTCGTG CACGAG(-5/-1)
Bst1107I 376 GTATAC GTA^TAC
BstAPI 318 GCAGTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 480 CGCG CG^CG
BstZ17I 376 GTATAC GTA^TAC
Bsu36I 866 CCTCAGG CC^T[ACGT]AGG
DrdI 1489 GACCCCGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 866 CCTCAGG CC^T[ACGT]AGG
FauI 557 GCGGG CCCGC(4/6)
FbaI 434 TGATCA T^GATCA
Hin1I 1777 GACGTC G[GA]^CG[CT]C
HincII 1780 GTCAAC GT[CT]^[GA]AC
HindII 1780 GTCAAC GT[CT]^[GA]AC
KspI 479 CCGCGG CCGC^GG
MaeI 530 CTAG C^TAG
MvnI 480 CGCG CG^CG
NcoI 1010 CCATGG C^CATGG
NheI 529 GCTAGC G^CTAGC
NspI 290 ACATGT [GA]CATG^[CT]
PleI 693 GACTC GAGTC(4/5)
PpuMI 626 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1058 TCATGA T^CATGA
SacII 479 CCGCGG CCGC^GG
ScaI 512 AGTACT AGT^ACT
SexAI 1471 ACCTGGT A^CC[AT]GGT
SmaI 566 CCCGGG CCC^GGG
XmaCI 566 CCCGGG C^CCGGG
XmaI 566 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 286 GTAGAC GT^[AC][GT]AC
376 GTATAC
AflIII 290 ACATGT A^C[GA][CT]GT
1610 ACACGT
Aor51HI 209 AGCGCT AGC^GCT
450 AGCGCT
Asp700I 207 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
510 GAAGTACTTC
BanI 301 GGTGCC G^G[CT][GA]CC
1243 GGTGCC
BbsI 1764 GAAGAC GAAGAC(2/6)
1201 GTCTTC
BglI 915 GCCCACAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1133 GCCAGGGCGGC
BpuAI 1764 GAAGAC GAAGAC(2/6)
1201 GTCTTC
BsaWI 536 ACCGGA [AT]^CCGG[AT]
1160 TCCGGT
BsgI 827 GTGCAG GTGCAG(16/14)
1312 GTGCAG
BspMI 1178 ACCTGC ACCTGC(4/8)
298 GCAGGT
BstEII 180 GGTCACC G^GT[ACGT]ACC
1398 GGTCACC
BstPI 180 GGTCACC G^GT[ACGT]ACC
1398 GGTCACC
DraII 626 GGGTCCT [GA]G^G[ACGT]CC[CT]
1253 GGGGCCT
Eco47III 209 AGCGCT AGC^GCT
450 AGCGCT
EcoO109I 626 GGGTCCT [GA]G^G[ACGT]CC[CT]
1253 GGGGCCT
EcoO65I 180 GGTCACC G^GT[ACGT]ACC
1398 GGTCACC
HgaI 429 GACGC GACGC(5/10)
1583 GCGTC
MseI 116 TTAA T^TAA
986 TTAA
PflMI 1103 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1667 CCATCCTGTGG
PvuII 552 CAGCTG CAG^CTG
1626 CAGCTG
SacI 1054 GAGCTC GAGCT^C
1708 GAGCTC
TaqI 1320 TCGA T^CGA
1592 TCGA
TfiI 227 GAATC G^A[AT]TC
1023 GAATC
Tru9I 116 TTAA T^TAA
986 TTAA
TthHB8I 1320 TCGA T^CGA
1592 TCGA
Van91I 1103 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1667 CCATCCTGTGG
XcmI 893 CCAAACTCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1001 CCATCTTCACCATGG
XmnI 207 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
510 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbvI BcnI BglII BmyI BsaJI
BsiHKAI BsiYI BslI BsmAI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TseI Tsp45I TspRI XhoII