Restriction map for
pFN21AE6492
(Circular) (Six-base) MAPSORT of: pFN21AE6492.seq Check: 1042 from: 1 to: 6538
pFN21AE6492 6538 bp
With 182 enzymes: SgfI *
March 18, 2015 14:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2294 2294
Size: 6538
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3786 4348 278
Size: 53 83 186 936 2250 562 2468
Fragments arranged by size:
2468 2250 936 562 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2292 2382 3881 1937
Size: 355 90 1499 4594
Fragments arranged by size:
4594 1499 355 90
Acc65I G'GTAC_C
Cuts at: 3859 3859
Size: 6538
AclI AA'CG_TT
Cuts at: 1563 4665 5038 1563
Size: 3102 373 3063
Fragments arranged by size:
3102 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2528 2777 4601 5985 6253 1632
Size: 896 249 1824 1384 268 1917
Fragments arranged by size:
1917 1824 1384 896 268 249
AfeI AGC'GCT
Cuts at: 2216 2457 2216
Size: 241 6297
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6341
AflIII A'CryG_T
Cuts at: 1203 2295 3615 5437 6414 1203
Size: 1092 1320 1822 977 1327
Fragments arranged by size:
1822 1327 1320 1092 977
AgeI A'CCGG_T
Cuts at: 5369 5369
Size: 6538
AhdI GACnn_n'nnGTC
Cuts at: 5267 5267
Size: 6538
AleI CACnn'nnGTG
Cuts at: 1166 3633 1166
Size: 2467 4071
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6538
AlwNI CAG_nnn'CTG
Cuts at: 2087 2437 2559 4281 5853 2087
Size: 350 122 1722 1572 2772
Fragments arranged by size:
2772 1722 1572 350 122
ApaLI G'TGCA_C
Cuts at: 2671 4595 5751 2671
Size: 1924 1156 3458
Fragments arranged by size:
3458 1924 1156
ApoI r'AATT_y
Cuts at: 1493 3847 4073 4225 6334 1493
Size: 2354 226 152 2109 1697
Fragments arranged by size:
2354 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5092 160
Size: 872 4060 1606
Fragments arranged by size:
4060 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 3042 3268 3863 1870
Size: 73 628 471 226 595 4545
Fragments arranged by size:
4545 628 595 471 226 73
BamHI G'GATC_C
Cuts at: 3117 3868 4235 3117
Size: 751 367 5420
Fragments arranged by size:
5420 751 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2306 3248 3859 5314 618
Size: 325 217 627 519 942 611 1455 1842
Fragments arranged by size:
1842 1455 942 627 611 519 325 217
BanII G_rGCy'C
Cuts at: 1391 2931 3063 3717 3857 1391
Size: 1540 132 654 140 4072
Fragments arranged by size:
4072 1540 654 140 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3199 3776 928
Size: 971 1300 577 3690
Fragments arranged by size:
3690 1300 971 577
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4729 4763 1903
Size: 34 2792 34 3678
Fragments arranged by size:
3678 2792 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2904 4430 5640 2904
Size: 1526 1210 3802
Fragments arranged by size:
3802 1526 1210
BclI T'GATC_A
Cuts at: 1548 2439 1548
Size: 891 5647
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2926 3144 5149
Size: 107 122 71 631 605 1254 218 2005
Cuts at: 5149 136
Size: 1525
Fragments arranged by size:
2005 1525 1254 631 605 218 122 107
71
BglII A'GATC_T
Cuts at: 2283 2844 2856 6533 2283
Size: 561 12 3677 2288
Fragments arranged by size:
3677 2288 561 12
BlpI GC'TnA_GC
Cuts at: 3955 3955
Size: 6538
Bme1580I G_kGCm'C
Cuts at: 1790 2675 4599 5755 1790
Size: 885 1924 1156 2573
Fragments arranged by size:
2573 1924 1156 885
BmgBI CAC'GTC
Cuts at: 1318 3618 1318
Size: 2300 4238
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4269 5227 442
Size: 416 53 83 399 24 2852 958 1753
Fragments arranged by size:
2852 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2538 1056
Size: 1482 5056
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3466 3466
Size: 6538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5198 1239
Size: 3959 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4663 5528 5826 4663
Size: 865 298 5375
Fragments arranged by size:
5375 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3109 5201 882
Size: 617 1610 2092 2219
Fragments arranged by size:
2219 2092 1610 617
BsaAI yAC'GTr
Cuts at: 493 1204 3316 4329 493
Size: 711 2112 1013 2702
Fragments arranged by size:
2702 2112 1013 711
BsaBI GATnn'nnATC
Cuts at: 6532 6532
Size: 6538
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3783 4345 4727 5362
Size: 53 83 186 936 2250 562 382 635
Cuts at: 5362 275
Size: 1451
Fragments arranged by size:
2250 1451 936 635 562 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2541 3165 4971 5369 5643 5790 6201 2541
Size: 624 1806 398 274 147 411 2878
Fragments arranged by size:
2878 1806 624 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6313
Fragments arranged by size:
6313 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6538
BseYI C'CCAG_C
Cuts at: 5741 6237 5741
Size: 496 6042
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2853 3338 1163
Size: 573 1117 485 4363
Fragments arranged by size:
4363 1117 573 485
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3988 4749 5777 1540
Size: 275 186 1987 761 1028 2301
Fragments arranged by size:
2301 1987 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2675 2740 3063 3717 4599 4684 5755 2675
Size: 65 323 654 882 85 1071 3458
Fragments arranged by size:
3458 1071 882 654 323 85 65
BsmI GAATG_Cn'
Cuts at: 3366 4049 4142 3366
Size: 683 93 5762
Fragments arranged by size:
5762 683 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2675 2740 2931 3063 3717 3857 4599
Size: 399 885 65 191 132 654 140 742
Cuts at: 4599 4684 5755 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 885 742 654 399 191 140
132 85 65
BspEI T'CCGG_A
Cuts at: 6201 6201
Size: 6538
BspHI T'CATG_A
Cuts at: 3063 4427 6157 3063
Size: 1364 1730 3444
Fragments arranged by size:
3444 1730 1364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2294 3192 3893 844
Size: 1450 898 701 3489
Fragments arranged by size:
3489 1450 898 701
BsrBI CCG'CTC
Cuts at: 1394 1823 4425 1394
Size: 429 2602 3507
Fragments arranged by size:
3507 2602 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2050 2172 2886 5033 5207 65
Size: 1183 802 122 714 2147 174 1396
Fragments arranged by size:
2147 1396 1183 802 714 174 122
BsrFI r'CCGG_y
Cuts at: 1700 1810 5182 5369 1700
Size: 110 3372 187 2869
Fragments arranged by size:
3372 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6538
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6538
BssSI C'ACGA_G
Cuts at: 2903 4598 5610 2903
Size: 1695 1012 3831
Fragments arranged by size:
3831 1695 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2329 794
Size: 1535 5003
BstBI TT'CG_AA
Cuts at: 5342 5342
Size: 6538
BstEII G'GTnAC_C
Cuts at: 1184 2185 3403 1184
Size: 1001 1218 4319
Fragments arranged by size:
4319 1218 1001
BstXI CCAn_nnnn'nTGG
Cuts at: 2245 2661 3460 2245
Size: 416 799 5323
Fragments arranged by size:
5323 799 416
BstYI r'GATC_y
Cuts at: 1972 2154 2283 2338 2599 2836 2844 2856 3117
Size: 182 129 55 261 237 8 12 261
Cuts at: 3117 3868 4235 4620 4637 6089 6175 6187 6533
Size: 751 367 385 17 1452 86 12 346
Cuts at: 6533 1972
Size: 1977
Fragments arranged by size:
1977 1452 751 385 367 346 261 261
237 182 129 86 55 17 12 12
8
BstZ17I GTA'TAC
Cuts at: 2383 2383
Size: 6538
Bsu36I CC'TnA_GG
Cuts at: 2872 2872
Size: 6538
BtgI C'CryG_G
Cuts at: 513 1065 2004 2484 2621 2932 3015 3399 3498
Size: 552 939 480 137 311 83 384 99
Cuts at: 3498 513
Size: 3553
Fragments arranged by size:
3553 939 552 480 384 311 137 99
83
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5799
Fragments arranged by size:
5799 683 56
BtsI GCAGTG_nn'
Cuts at: 2373 4054 4848 4868 2373
Size: 1681 794 20 4043
Fragments arranged by size:
4043 1681 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6538
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6503
DraI TTT'AAA
Cuts at: 3842 3842
Size: 6538
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3500 5545 809
Size: 507 2184 2045 1802
Fragments arranged by size:
2184 2045 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2015 2349 2367
Size: 54 1425 50 275 18 185 334 18
Cuts at: 2367 2624 3145 3720 3985 4874 6503 8
Size: 257 521 575 265 889 1629 43
Fragments arranged by size:
1629 1425 889 575 521 334 275 265
257 185 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 3985 1537
Size: 275 2173 4090
Fragments arranged by size:
4090 2173 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2777 2816 3158 3224 3356 3705 4468 1353
Size: 1424 39 342 66 132 349 763 3423
Fragments arranged by size:
3423 1424 763 349 342 132 66 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5125 5499 5645 387
Size: 1064 138 3536 374 146 1280
Fragments arranged by size:
3536 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3061 3715 3061
Size: 654 5884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2528 2777 4601 5198 5985 6253 1239
Size: 393 896 249 1824 597 787 268 1524
Fragments arranged by size:
1824 1524 896 787 597 393 268 249
EcoO109I rG'GnC_Cy
Cuts at: 2632 3259 2632
Size: 627 5911
EcoRI G'AATT_C
Cuts at: 3847 4225 3847
Size: 378 6160
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2539 3268 2539
Size: 729 5809
FspI TGC'GCA
Cuts at: 5044 5044
Size: 6538
HaeII r_GCGC'y
Cuts at: 1678 2218 2459 3349 5685 1678
Size: 540 241 890 2336 2531
Fragments arranged by size:
2531 2336 890 540 241
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2599 2631 5179 5211 5253
Size: 32 135 32 1027 32 2548 32 42
Cuts at: 5253 5285 1373
Size: 32 2626
Fragments arranged by size:
2626 2548 1027 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3787 3882 4128 669
Size: 988 281 1849 95 246 3079
Fragments arranged by size:
3079 1849 988 281 246 95
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6538
HpaI GTT'AAC
Cuts at: 4128 4128
Size: 6538
KpnI G_GTAC'C
Cuts at: 3863 3863
Size: 6538
MfeI C'AATT_G
Cuts at: 4137 4137
Size: 6538
MluI A'CGCG_T
Cuts at: 6414 6414
Size: 6538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2029 5652 5836 1305
Size: 724 3623 184 2007
Fragments arranged by size:
3623 2007 724 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2017 2351 2369 10
Size: 54 1425 343 185 334 18 4179
Fragments arranged by size:
4179 1425 343 334 185 54 18
MslI CAynn'nnrTG
Cuts at: 518 1166 2192 2465 2780 3068 3633 4496 4855
Size: 648 1026 273 315 288 565 863 359
Cuts at: 4855 5014 518
Size: 159 2042
Fragments arranged by size:
2042 1026 863 648 565 359 315 288
273 159
MspA1I CmG'CkG
Cuts at: 1606 2486 2559 2605 2695 3633 3954 4631 5779
Size: 880 73 46 90 938 321 677 1148
Cuts at: 5779 6024 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 938 880 677 321 245 90
73 46
NcoI C'CATG_G
Cuts at: 513 1065 2004 3015 513
Size: 552 939 1011 4036
Fragments arranged by size:
4036 1011 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6538
NheI G'CTAG_C
Cuts at: 1052 2534 1052
Size: 1482 5056
NotI GC'GGCC_GC
Cuts at: 3985 3985
Size: 6538
NspI r_CATG'y
Cuts at: 1132 1229 2299 3896 5441 1132
Size: 97 1070 1597 1545 2229
Fragments arranged by size:
2229 1597 1545 1070 97
PasI CC'CwG_GG
Cuts at: 1348 2789 1348
Size: 1441 5097
PciI A'CATG_T
Cuts at: 2295 5437 2295
Size: 3142 3396
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 3114 3678 1108
Size: 902 1104 564 3968
Fragments arranged by size:
3968 1104 902 564
PfoI T'CCnGG_A
Cuts at: 3300 3777 3300
Size: 477 6061
PmeI GTTT'AAAC
Cuts at: 3842 3842
Size: 6538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4607 4639 6120 6152 4607
Size: 32 1481 32 4993
Fragments arranged by size:
4993 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2632 2632
Size: 6538
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6538
PsiI TTA'TAA
Cuts at: 4108 4108
Size: 6538
PstI C_TGCA'G
Cuts at: 830 2433 2864 3189 3890 830
Size: 1603 431 325 701 3478
Fragments arranged by size:
3478 1603 701 431 325
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6538
PvuII CAG'CTG
Cuts at: 2559 3633 2559
Size: 1074 5464
SacI G_AGCT'C
Cuts at: 3063 3717 3063
Size: 654 5884
SacII CC_GC'GG
Cuts at: 2487 2487
Size: 6538
SalI G'TCGA_C
Cuts at: 1936 3880 1936
Size: 1944 4594
SapI GCTCTTCn'nnn_
Cuts at: 3158 3356 3705 3158
Size: 198 349 5991
Fragments arranged by size:
5991 349 198
SbfI CC_TGCA'GG
Cuts at: 3890 3890
Size: 6538
ScaI AGT'ACT
Cuts at: 2519 4786 6498 2519
Size: 2267 1712 2559
Fragments arranged by size:
2559 2267 1712
SexAI A'CCwGG_T
Cuts at: 3476 3476
Size: 6538
SfcI C'TryA_G
Cuts at: 826 1045 2429 2473 2860 3185 3886 5021 5702
Size: 219 1384 44 387 325 701 1135 681
Cuts at: 5702 5893 826
Size: 191 1471
Fragments arranged by size:
1471 1384 1135 701 681 387 325 219
191 44
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6538
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6538
SmaI CCC'GGG
Cuts at: 1872 2573 3865 1872
Size: 701 1292 4545
Fragments arranged by size:
4545 1292 701
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4642 5543 5805 820
Size: 197 926 2699 901 262 1553
Fragments arranged by size:
2699 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4329 493
Size: 3836 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6538
SphI G_CATG'C
Cuts at: 1132 3896 1132
Size: 2764 3774
SspI AAT'ATT
Cuts at: 5 52 4462 5
Size: 47 4410 2081
Fragments arranged by size:
4410 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6538
StyI C'CwwG_G
Cuts at: 513 1065 2004 3015 3453 3762 3977 513
Size: 552 939 1011 438 309 215 3074
Fragments arranged by size:
3074 1011 939 552 438 309 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2012 4565 4582 4735 4920 5365 325
Size: 1255 432 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 432 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2251 2517 4784
Size: 274 80 33 51 1443 274 266 2267
Cuts at: 4784 6496 96
Size: 1712 138
Fragments arranged by size:
2267 1712 1443 274 274 266 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6498 6530 6498
Size: 32 6506
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6538
XbaI T'CTAG_A
Cuts at: 3874 3874
Size: 6538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2905 3013 2905
Size: 108 6430
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6538
XmaI C'CCGG_G
Cuts at: 1870 2571 3863 1870
Size: 701 1292 4545
Fragments arranged by size:
4545 1292 701
XmnI GAAnn'nnTTC
Cuts at: 2216 2519 4667 2216
Size: 303 2148 4087
Fragments arranged by size:
4087 2148 303
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3784 4346 276
Size: 53 83 186 936 2250 562 2468
Fragments arranged by size:
2468 2250 936 562 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI DraIII EcoNI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI
SwaI