(Linear) (Six-base) MAPSORT of: KIEE6492.seq Check: 3693 from: 1 to: 1833 KIEE6492.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 289 1833 Size: 289 1544 AatII G_ACGT'C Cuts at: 0 1781 1833 Size: 1781 52 AccI GT'mk_AC Cuts at: 0 287 377 1833 Size: 287 90 1456 Fragments arranged by size: 1456 287 90 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 772 1833 Size: 523 249 1061 Fragments arranged by size: 1061 523 249 AfeI AGC'GCT Cuts at: 0 211 452 1833 Size: 211 241 1381 Fragments arranged by size: 1381 241 211 AflIII A'CryG_T Cuts at: 0 290 1610 1833 Size: 290 1320 223 Fragments arranged by size: 1320 290 223 AleI CACnn'nnGTG Cuts at: 0 1628 1833 Size: 1628 205 AlwNI CAG_nnn'CTG Cuts at: 0 82 432 554 1833 Size: 82 350 122 1279 Fragments arranged by size: 1279 350 122 82 ApaLI G'TGCA_C Cuts at: 0 666 1833 Size: 666 1167 AvaI C'yCGr_G Cuts at: 0 566 1037 1263 1833 Size: 566 471 226 570 Fragments arranged by size: 570 566 471 226 BamHI G'GATC_C Cuts at: 0 1112 1833 Size: 1112 721 BanI G'GyrC_C Cuts at: 0 301 1243 1833 Size: 301 942 590 Fragments arranged by size: 942 590 301 BanII G_rGCy'C Cuts at: 0 926 1058 1712 1833 Size: 926 132 654 121 Fragments arranged by size: 926 654 132 121 BbsI GAAGACnn'nnnn_ Cuts at: 0 1194 1771 1833 Size: 1194 577 62 Fragments arranged by size: 1194 577 62 BciVI GTATCCnnnnn_n' Cuts at: 0 899 1833 Size: 899 934 BclI T'GATC_A Cuts at: 0 434 1833 Size: 434 1399 BglI GCCn_nnn'nGGC Cuts at: 0 921 1139 1833 Size: 921 218 694 Fragments arranged by size: 921 694 218 BglII A'GATC_T Cuts at: 0 278 839 851 1833 Size: 278 561 12 982 Fragments arranged by size: 982 561 278 12 Bme1580I G_kGCm'C Cuts at: 0 670 1833 Size: 670 1163 BmgBI CAC'GTC Cuts at: 0 1613 1833 Size: 1613 220 BmtI G_CTAG'C Cuts at: 0 533 1833 Size: 533 1300 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1461 1833 Size: 1461 372 BsaI GGTCTCn'nnnn_ Cuts at: 0 1104 1833 Size: 1104 729 BsaAI yAC'GTr Cuts at: 0 1311 1833 Size: 1311 522 BsaHI Gr'CG_yC Cuts at: 0 1778 1833 Size: 1778 55 BsaWI w'CCGG_w Cuts at: 0 536 1160 1833 Size: 536 624 673 Fragments arranged by size: 673 624 536 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 848 1333 1833 Size: 848 485 500 Fragments arranged by size: 848 500 485 BsiHKAI G_wGCw'C Cuts at: 0 670 735 1058 1712 1833 Size: 670 65 323 654 121 Fragments arranged by size: 670 654 323 121 65 BsmI GAATG_Cn' Cuts at: 0 1361 1833 Size: 1361 472 Bsp1286I G_dGCh'C Cuts at: 0 670 735 926 1058 1712 1833 Size: 670 65 191 132 654 121 Fragments arranged by size: 670 654 191 132 121 65 BspHI T'CATG_A Cuts at: 0 1058 1833 Size: 1058 775 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 289 1187 1833 Size: 289 898 646 Fragments arranged by size: 898 646 289 BsrDI GCAATG_nn' Cuts at: 0 45 167 881 1833 Size: 45 122 714 952 Fragments arranged by size: 952 714 122 45 BssSI C'ACGA_G Cuts at: 0 898 1833 Size: 898 935 BstAPI GCAn_nnn'nTGC Cuts at: 0 324 1833 Size: 324 1509 BstEII G'GTnAC_C Cuts at: 0 180 1398 1833 Size: 180 1218 435 Fragments arranged by size: 1218 435 180 BstXI CCAn_nnnn'nTGG Cuts at: 0 240 656 1455 1833 Size: 240 416 799 378 Fragments arranged by size: 799 416 378 240 BstYI r'GATC_y Cuts at: 0 149 278 333 594 831 839 851 1112 Size: 149 129 55 261 237 8 12 261 Cuts at: 1112 1833 Size: 721 Fragments arranged by size: 721 261 261 237 149 129 55 12 8 BstZ17I GTA'TAC Cuts at: 0 378 1833 Size: 378 1455 Bsu36I CC'TnA_GG Cuts at: 0 867 1833 Size: 867 966 BtgI C'CryG_G Cuts at: 0 479 616 927 1010 1394 1493 1833 Size: 479 137 311 83 384 99 340 Fragments arranged by size: 479 384 340 311 137 99 83 BtsI GCAGTG_nn' Cuts at: 0 368 1833 Size: 368 1465 DrdI GACnn_nn'nnGTC Cuts at: 0 1495 1833 Size: 1495 338 EaeI y'GGCC_r Cuts at: 0 10 344 362 619 1140 1715 1833 Size: 10 334 18 257 521 575 118 Fragments arranged by size: 575 521 334 257 118 18 10 EarI CTCTTCn'nnn_ Cuts at: 0 772 811 1153 1219 1351 1700 1833 Size: 772 39 342 66 132 349 133 Fragments arranged by size: 772 349 342 133 132 66 39 EcoICRI GAG'CTC Cuts at: 0 1056 1710 1833 Size: 1056 654 123 Fragments arranged by size: 1056 654 123 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 523 772 1833 Size: 523 249 1061 Fragments arranged by size: 1061 523 249 EcoO109I rG'GnC_Cy Cuts at: 0 627 1254 1833 Size: 627 627 579 Fragments arranged by size: 627 627 579 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 534 1263 1833 Size: 534 729 570 Fragments arranged by size: 729 570 534 HaeII r_GCGC'y Cuts at: 0 213 454 1344 1833 Size: 213 241 890 489 Fragments arranged by size: 890 489 241 213 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 594 626 1833 Size: 594 32 1207 Fragments arranged by size: 1207 594 32 HincII GTy'rAC Cuts at: 0 1782 1833 Size: 1782 51 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 24 1833 Size: 24 1809 MscI TGG'CCA Cuts at: 0 12 346 364 1833 Size: 12 334 18 1469 Fragments arranged by size: 1469 334 18 12 MslI CAynn'nnrTG Cuts at: 0 187 460 775 1063 1628 1833 Size: 187 273 315 288 565 205 Fragments arranged by size: 565 315 288 273 205 187 MspA1I CmG'CkG Cuts at: 0 481 554 600 690 1628 1833 Size: 481 73 46 90 938 205 Fragments arranged by size: 938 481 205 90 73 46 NcoI C'CATG_G Cuts at: 0 1010 1833 Size: 1010 823 NheI G'CTAG_C Cuts at: 0 529 1833 Size: 529 1304 NspI r_CATG'y Cuts at: 0 294 1833 Size: 294 1539 PasI CC'CwG_GG Cuts at: 0 784 1833 Size: 784 1049 PciI A'CATG_T Cuts at: 0 290 1833 Size: 290 1543 PflMI CCAn_nnn'nTGG Cuts at: 0 1109 1673 1833 Size: 1109 564 160 Fragments arranged by size: 1109 564 160 PfoI T'CCnGG_A Cuts at: 0 1295 1772 1833 Size: 1295 477 61 Fragments arranged by size: 1295 477 61 PpuMI rG'GwC_Cy Cuts at: 0 627 1833 Size: 627 1206 PstI C_TGCA'G Cuts at: 0 428 859 1184 1833 Size: 428 431 325 649 Fragments arranged by size: 649 431 428 325 PvuII CAG'CTG Cuts at: 0 554 1628 1833 Size: 554 1074 205 Fragments arranged by size: 1074 554 205 SacI G_AGCT'C Cuts at: 0 1058 1712 1833 Size: 1058 654 121 Fragments arranged by size: 1058 654 121 SacII CC_GC'GG Cuts at: 0 482 1833 Size: 482 1351 SapI GCTCTTCn'nnn_ Cuts at: 0 1153 1351 1700 1833 Size: 1153 198 349 133 Fragments arranged by size: 1153 349 198 133 ScaI AGT'ACT Cuts at: 0 514 1833 Size: 514 1319 SexAI A'CCwGG_T Cuts at: 0 1471 1833 Size: 1471 362 SfcI C'TryA_G Cuts at: 0 424 468 855 1180 1833 Size: 424 44 387 325 653 Fragments arranged by size: 653 424 387 325 44 SmaI CCC'GGG Cuts at: 0 568 1833 Size: 568 1265 StyI C'CwwG_G Cuts at: 0 1010 1448 1757 1833 Size: 1010 438 309 76 Fragments arranged by size: 1010 438 309 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 7 1833 Size: 7 1826 TatI w'GTAC_w Cuts at: 0 246 512 1833 Size: 246 266 1321 Fragments arranged by size: 1321 266 246 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 900 1008 1833 Size: 900 108 825 Fragments arranged by size: 900 825 108 XmaI C'CCGG_G Cuts at: 0 566 1833 Size: 566 1267 XmnI GAAnn'nnTTC Cuts at: 0 211 514 1833 Size: 211 303 1319 Fragments arranged by size: 1319 303 211 ZraI GAC'GTC Cuts at: 0 1779 1833 Size: 1779 54 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AleI AlwNI ApaLI AvaI BamHI BanI BanII BbsI BciVI BclI BglI BglII Bme1580I BmgBI BmtI BplI BsaI BsaAI BsaHI BsaWI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I NcoI NheI NspI PasI PciI PflMI PfoI PpuMI PstI PvuII SacI SacII SapI ScaI SexAI SfcI SmaI StyI TaqII TatI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AflII AgeI AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BfrBI BlpI BmrI BpmI Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstBI BtgZI ClaI CspCI DraI DraIII EagI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI