Restriction map for
pFN21AE1718
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstYI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI Fnu4HI FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaII ItaI KasI
KpnI KspI MaeI MaeII MamI
MfeI MflI MluI MroI MslI
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I StuI StyI
SwaI TseI Tsp509I Tth111I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AlwI 368 GGATC GGATC(4/5)
AlwNI 502 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 362 CCTCAGG CC^T[ACGT]AGG
ApaI 586 GGGCCC GGGCC^C
BalI 51 TGGCCA TGG^CCA
BanII 586 GGGCCC G[GA]GC[CT]^C
BpmI 329 CTCCAG CTGGAG(16/14)
Bsp1407I 343 TGTACA T^GTACA
BsrGI 343 TGTACA T^GTACA
Bsu36I 362 CCTCAGG CC^T[ACGT]AGG
DpnII 369 GATC ^GATC
DraII 586 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 153 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 57 GACAGACATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 192 CCGTGG C^C[GA][CT]GG
EaeI 51 TGGCCA [CT]^GGCC[GA]
EarI 408 GAAGAG CTCTTC(1/4)
Eco81I 362 CCTCAGG CC^T[ACGT]AGG
EcoO109I 586 GGGCCCT [GA]G^G[ACGT]CC[CT]
HincII 129 GTTAAC GT[CT]^[GA]AC
HindII 129 GTTAAC GT[CT]^[GA]AC
HpaI 129 GTTAAC GTT^AAC
Ksp632I 408 GAAGAG CTCTTC(1/4)
MboI 369 GATC ^GATC
MluNI 51 TGGCCA TGG^CCA
MscI 51 TGGCCA TGG^CCA
MspA1I 661 CAGCTG C[AC]G^C[GT]G
NdeII 369 GATC ^GATC
PflMI 501 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 661 CAGCTG CAG^CTG
Sau3AI 369 GATC ^GATC
SfaNI 449 GCATC GCATC(5/9)
SspBI 343 TGTACA T^GTACA
SspI 297 AATATT AAT^ATT
Van91I 501 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 62 ACATGT A^C[GA][CT]GT
533 ACATGT
BmyI 586 GGGCCC G[AGT]GC[ACT]^C
617 GGGCAC
BsmAI 225 GAGAC GTCTC(1/5)
515 GAGAC
BsmFI 539 GGGAC GGGAC(10/14)
504 GTCCC
Bsp1286I 586 GGGCCC G[AGT]GC[ACT]^C
617 GGGCAC
BspLU11I 62 ACATGT A^CATGT
533 ACATGT
BssKI 398 CCAGG ^CC[ACGT]GG
507 CCTGG
BstNI 398 CCAGG CC^[AT]GG
507 CCTGG
DdeI 257 CTAAG C^T[ACGT]AG
363 CTCAG
EcoRII 398 CCAGG ^CC[AT]GG
507 CCTGG
FauI 584 GCGGG CCCGC(4/6)
615 GCGGG
MaeIII 319 GTCAC ^GT[ACGT]AC
552 GTCAC
MboII 408 GAAGA GAAGA(8/7)
417 GAAGA
MseI 130 TTAA T^TAA
566 TTAA
MvaI 398 CCAGG CC^[AT]GG
507 CCTGG
NlaIV 309 GGTTCC GG[ACGT]^[ACGT]CC
586 GGGCCC
NspI 62 ACATGT [GA]CATG^[CT]
533 ACATGT
ScrFI 398 CCAGG CC^[ACGT]GG
507 CCTGG
TfiI 240 GATTC G^A[AT]TC
472 GATTC
Tru9I 130 TTAA T^TAA
566 TTAA
Tsp45I 319 GTCAC ^GT[GC]AC
552 GTCAC
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# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AfaI AluI BciVI BsaJI
BsiYI BslI BsrI Cac8I Cfr13I
HaeIII HinfI HphI MnlI NlaIII
PleI RsaI Sau96I TaqI TspRI
TthHB8I