(Circular) (Six-base) MAPSORT of: pFN21AE1718.seq Check: 2923 from: 1 to: 5377 pFN21AE1718 5377 bp With 182 enzymes: SgfI * January 30, 2014 17:16 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3187 278 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2720 1937 Size: 783 4594 Acc65I G'GTAC_C Cuts at: 2698 2698 Size: 5377 AclI AA'CG_TT Cuts at: 1563 3504 3877 1563 Size: 1941 373 3063 Fragments arranged by size: 3063 1941 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2441 2581 2648 3440 4824 5092 1632 Size: 708 101 140 67 792 1384 268 1917 Fragments arranged by size: 1917 1384 792 708 268 140 101 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5180 AflIII A'CryG_T Cuts at: 1203 2067 2538 4276 5253 1203 Size: 864 471 1738 977 1327 Fragments arranged by size: 1738 1327 977 864 471 AgeI A'CCGG_T Cuts at: 4208 4208 Size: 5377 AhdI GACnn_n'nnGTC Cuts at: 4106 4106 Size: 5377 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5377 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5377 AlwNI CAG_nnn'CTG Cuts at: 2512 3120 4692 2512 Size: 608 1572 3197 Fragments arranged by size: 3197 1572 608 ApaI G_GGCC'C Cuts at: 2595 2595 Size: 5377 ApaLI G'TGCA_C Cuts at: 3434 4590 3434 Size: 1156 4221 ApoI r'AATT_y Cuts at: 1493 2686 2912 3064 5173 1493 Size: 1193 226 152 2109 1697 Fragments arranged by size: 2109 1697 1193 226 152 AseI AT'TA_AT Cuts at: 160 1032 3931 160 Size: 872 2899 1606 Fragments arranged by size: 2899 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 AvaI C'yCGr_G Cuts at: 1870 1943 2702 1870 Size: 73 759 4545 Fragments arranged by size: 4545 759 73 BamHI G'GATC_C Cuts at: 2707 3074 2707 Size: 367 5010 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2698 4153 618 Size: 325 217 627 911 1455 1842 Fragments arranged by size: 1842 1455 911 627 325 217 BanII G_rGCy'C Cuts at: 1391 2595 2696 1391 Size: 1204 101 4072 Fragments arranged by size: 4072 1204 101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4406 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3568 3602 1903 Size: 34 1631 34 3678 Fragments arranged by size: 3678 1631 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2098 2128 2267 2423 3269 4479 2098 Size: 30 139 156 846 1210 2996 Fragments arranged by size: 2996 1210 846 156 139 30 BclI T'GATC_A Cuts at: 1548 1548 Size: 5377 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3988 136 Size: 107 122 71 631 605 2316 1525 Fragments arranged by size: 2316 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5372 5372 Size: 5377 BlpI GC'TnA_GC Cuts at: 2794 2794 Size: 5377 Bme1580I G_kGCm'C Cuts at: 1790 2595 2626 3438 4594 1790 Size: 805 31 812 1156 2573 Fragments arranged by size: 2573 1156 812 805 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5377 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3108 4066 442 Size: 416 53 83 399 24 1691 958 1753 Fragments arranged by size: 1753 1691 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5377 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2383 Size: 5377 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2319 4037 1239 Size: 1080 1718 2579 Fragments arranged by size: 2579 1718 1080 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3502 4367 4665 3502 Size: 865 298 4214 Fragments arranged by size: 4214 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4040 882 Size: 617 2541 2219 Fragments arranged by size: 2541 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3168 493 Size: 711 1964 2702 Fragments arranged by size: 2702 1964 711 BsaBI GATnn'nnATC Cuts at: 5371 5371 Size: 5377 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3184 3566 4201 275 Size: 53 83 186 936 1651 382 635 1451 Fragments arranged by size: 1651 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3810 4208 4482 4629 5040 3810 Size: 398 274 147 411 4147 Fragments arranged by size: 4147 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2000 2030 2317 2347 1178 Size: 30 165 30 597 30 287 30 4208 Fragments arranged by size: 4208 597 287 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5377 BseYI C'CCAG_C Cuts at: 2355 2664 4580 5076 2355 Size: 309 1916 496 2656 Fragments arranged by size: 2656 1916 496 309 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4804 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2827 3588 4616 1540 Size: 275 186 826 761 1028 2301 Fragments arranged by size: 2301 1028 826 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3438 3523 4594 3438 Size: 85 1071 4221 Fragments arranged by size: 4221 1071 85 BsmI GAATG_Cn' Cuts at: 2888 2981 2888 Size: 93 5284 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2595 2626 2696 3438 3523 4594 1391 Size: 399 805 31 70 742 85 1071 2174 Fragments arranged by size: 2174 1071 805 742 399 85 70 31 BspEI T'CCGG_A Cuts at: 5040 5040 Size: 5377 BspHI T'CATG_A Cuts at: 3266 4996 3266 Size: 1730 3647 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2732 844 Size: 1888 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3264 1394 Size: 429 1441 3507 Fragments arranged by size: 3507 1441 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3872 4046 65 Size: 1183 2624 174 1396 Fragments arranged by size: 2624 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4021 4208 1700 Size: 110 2211 187 2869 Fragments arranged by size: 2869 2211 187 110 BsrGI T'GTAC_A Cuts at: 96 2348 96 Size: 2252 3125 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5377 BssSI C'ACGA_G Cuts at: 3437 4449 3437 Size: 1012 4365 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5377 BstBI TT'CG_AA Cuts at: 4181 4181 Size: 5377 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5377 BstYI r'GATC_y Cuts at: 1972 2707 3074 3459 3476 4928 5014 5026 5372 Size: 735 367 385 17 1452 86 12 346 Cuts at: 5372 1972 Size: 1977 Fragments arranged by size: 1977 1452 735 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2368 2368 Size: 5377 BtgI C'CryG_G Cuts at: 513 1065 2004 2197 513 Size: 552 939 193 3693 Fragments arranged by size: 3693 939 552 193 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4638 Fragments arranged by size: 4638 683 56 BtsI GCAGTG_nn' Cuts at: 2893 3687 3707 2893 Size: 794 20 4563 Fragments arranged by size: 4563 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5377 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5342 DraI TTT'AAA Cuts at: 2681 2681 Size: 5377 DraIII CAC_nnn'GTG Cuts at: 2163 2163 Size: 5377 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2068 4384 809 Size: 507 752 2316 1802 Fragments arranged by size: 2316 1802 752 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 2824 3713 Size: 54 1425 50 275 18 226 768 889 Cuts at: 3713 5342 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 768 275 226 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2824 1537 Size: 275 1012 4090 Fragments arranged by size: 4090 1012 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2408 3307 1353 Size: 1055 899 3423 Fragments arranged by size: 3423 1055 899 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2353 3964 4338 4484 387 Size: 1064 138 764 1611 374 146 1280 Fragments arranged by size: 1611 1280 1064 764 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2319 2340 2441 2581 2648 3440 4037 Size: 393 687 21 101 140 67 792 597 Cuts at: 4037 4824 5092 1239 Size: 787 268 1524 Fragments arranged by size: 1524 792 787 687 597 393 268 140 101 67 21 EcoO109I rG'GnC_Cy Cuts at: 2592 2592 Size: 5377 EcoRI G'AATT_C Cuts at: 2686 3064 2686 Size: 378 4999 FspI TGC'GCA Cuts at: 3883 3883 Size: 5377 HaeII r_GCGC'y Cuts at: 1678 4524 1678 Size: 2846 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2401 2433 4018 4050 4092 Size: 32 135 32 829 32 1585 32 42 Cuts at: 4092 4124 1373 Size: 32 2626 Fragments arranged by size: 2626 1585 829 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2136 2721 2967 669 Size: 988 281 198 585 246 3079 Fragments arranged by size: 3079 988 585 281 246 198 HindIII A'AGCT_T Cuts at: 748 748 Size: 5377 HpaI GTT'AAC Cuts at: 2136 2967 2136 Size: 831 4546 KpnI G_GTAC'C Cuts at: 2702 2702 Size: 5377 MfeI C'AATT_G Cuts at: 2976 2976 Size: 5377 MluI A'CGCG_T Cuts at: 5253 5253 Size: 5377 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2082 4491 4675 1305 Size: 777 2409 184 2007 Fragments arranged by size: 2409 2007 777 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2058 10 Size: 54 1425 343 226 3329 Fragments arranged by size: 3329 1425 343 226 54 MslI CAynn'nnrTG Cuts at: 518 1166 3335 3694 3853 518 Size: 648 2169 359 159 2042 Fragments arranged by size: 2169 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2668 2793 3470 4618 4863 1606 Size: 1062 125 677 1148 245 2120 Fragments arranged by size: 2120 1148 1062 677 245 125 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3886 Fragments arranged by size: 3886 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5377 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5377 NotI GC'GGCC_GC Cuts at: 2824 2824 Size: 5377 NspI r_CATG'y Cuts at: 1132 1229 2071 2542 2735 4280 1132 Size: 97 842 471 193 1545 2229 Fragments arranged by size: 2229 1545 842 471 193 97 PasI CC'CwG_GG Cuts at: 1348 2403 1348 Size: 1055 4322 PciI A'CATG_T Cuts at: 2067 2538 4276 2067 Size: 471 1738 3168 Fragments arranged by size: 3168 1738 471 PflMI CCAn_nnn'nTGG Cuts at: 1108 2512 1108 Size: 1404 3973 PmeI GTTT'AAAC Cuts at: 2681 2681 Size: 5377 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3446 3478 4959 4991 3446 Size: 32 1481 32 3832 Fragments arranged by size: 3832 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5377 PsiI TTA'TAA Cuts at: 2947 2947 Size: 5377 PspOMI G'GGCC_C Cuts at: 2591 2591 Size: 5377 PstI C_TGCA'G Cuts at: 830 2729 830 Size: 1899 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5377 PvuII CAG'CTG Cuts at: 2668 2668 Size: 5377 SalI G'TCGA_C Cuts at: 1936 2719 1936 Size: 783 4594 SbfI CC_TGCA'GG Cuts at: 2729 2729 Size: 5377 ScaI AGT'ACT Cuts at: 3625 5337 3625 Size: 1712 3665 SfcI C'TryA_G Cuts at: 826 1045 2725 3860 4541 4732 826 Size: 219 1680 1135 681 191 1471 Fragments arranged by size: 1680 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5377 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5377 SmaI CCC'GGG Cuts at: 1872 2704 1872 Size: 832 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3481 4382 4644 820 Size: 197 926 1538 901 262 1553 Fragments arranged by size: 1553 1538 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3168 493 Size: 2675 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5377 SphI G_CATG'C Cuts at: 1132 2735 1132 Size: 1603 3774 SspI AAT'ATT Cuts at: 5 52 2304 3301 5 Size: 47 2252 997 2081 Fragments arranged by size: 2252 2081 997 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5377 StyI C'CwwG_G Cuts at: 513 1065 2004 2816 513 Size: 552 939 812 3074 Fragments arranged by size: 3074 939 812 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2279 3404 3421 3574 3759 4204 325 Size: 1255 699 1125 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1125 699 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2338 2348 3623 Size: 274 80 33 51 1443 361 10 1275 Cuts at: 3623 5335 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1275 361 274 138 80 51 33 10 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2317 2349 2421 2453 5337 5369 2317 Size: 32 72 32 2884 32 2325 Fragments arranged by size: 2884 2325 72 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5377 XbaI T'CTAG_A Cuts at: 2713 2713 Size: 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5377 XmaI C'CCGG_G Cuts at: 1870 2702 1870 Size: 832 4545 XmnI GAAnn'nnTTC Cuts at: 3506 3506 Size: 5377 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3185 276 Size: 53 83 186 936 1651 2468 Fragments arranged by size: 2468 1651 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI