Restriction map for
pFN21AE1718
(Linear) (Six-base) MAPSORT of: KIEE1718.seq Check: 3699 from: 1 to: 672
KIEE1718.orf
With 182 enzymes: SgfI *
January 29, 2014 11:44 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 436 576 643 672
Size: 335 101 140 67 29
Fragments arranged by size:
335 140 101 67 29
AflIII A'CryG_T
Cuts at: 0 62 533 672
Size: 62 471 139
Fragments arranged by size:
471 139 62
AlwNI CAG_nnn'CTG
Cuts at: 0 507 672
Size: 507 165
ApaI G_GGCC'C
Cuts at: 0 590 672
Size: 590 82
BanII G_rGCy'C
Cuts at: 0 590 672
Size: 590 82
BciVI GTATCCnnnnn_n'
Cuts at: 0 93 123 262 418 672
Size: 93 30 139 156 254
Fragments arranged by size:
254 156 139 93 30
Bme1580I G_kGCm'C
Cuts at: 0 590 621 672
Size: 590 31 51
Fragments arranged by size:
590 51 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 378 672
Size: 378 294
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 672
Size: 314 358
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 312 342 672
Size: 25 287 30 330
Fragments arranged by size:
330 287 30 25
BseYI C'CCAG_C
Cuts at: 0 350 659 672
Size: 350 309 13
Fragments arranged by size:
350 309 13
Bsp1286I G_dGCh'C
Cuts at: 0 590 621 672
Size: 590 31 51
Fragments arranged by size:
590 51 31
BsrGI T'GTAC_A
Cuts at: 0 343 672
Size: 343 329
Bsu36I CC'TnA_GG
Cuts at: 0 363 672
Size: 363 309
BtgI C'CryG_G
Cuts at: 0 192 672
Size: 192 480
DraIII CAC_nnn'GTG
Cuts at: 0 158 672
Size: 158 514
DrdI GACnn_nn'nnGTC
Cuts at: 0 63 672
Size: 63 609
EaeI y'GGCC_r
Cuts at: 0 51 672
Size: 51 621
EarI CTCTTCn'nnn_
Cuts at: 0 403 672
Size: 403 269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 348 672
Size: 348 324
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 335 436 576 643 672
Size: 314 21 101 140 67 29
Fragments arranged by size:
314 140 101 67 29 21
EcoO109I rG'GnC_Cy
Cuts at: 0 587 672
Size: 587 85
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 672
Size: 396 32 244
Fragments arranged by size:
396 244 32
HincII GTy'rAC
Cuts at: 0 131 672
Size: 131 541
HpaI GTT'AAC
Cuts at: 0 131 672
Size: 131 541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 77 672
Size: 77 595
MscI TGG'CCA
Cuts at: 0 53 672
Size: 53 619
MspA1I CmG'CkG
Cuts at: 0 663 672
Size: 663 9
NspI r_CATG'y
Cuts at: 0 66 537 672
Size: 66 471 135
Fragments arranged by size:
471 135 66
PasI CC'CwG_GG
Cuts at: 0 398 672
Size: 398 274
PciI A'CATG_T
Cuts at: 0 62 533 672
Size: 62 471 139
Fragments arranged by size:
471 139 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 507 672
Size: 507 165
PspOMI G'GGCC_C
Cuts at: 0 586 672
Size: 586 86
PvuII CAG'CTG
Cuts at: 0 663 672
Size: 663 9
SspI AAT'ATT
Cuts at: 0 299 672
Size: 299 373
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 274 672
Size: 274 398
TatI w'GTAC_w
Cuts at: 0 333 343 672
Size: 333 10 329
Fragments arranged by size:
333 329 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 312 344 416 448 672
Size: 312 32 72 32 224
Fragments arranged by size:
312 224 72 32 32
Enzymes that do cut:
AcuI AflIII AlwNI ApaI BanII BciVI Bme1580I BplI
BpmI BsaXI BseYI Bsp1286I BsrGI Bsu36I BtgI DraIII
DrdI EaeI EarI EciI Eco57MI EcoO109I Hin4I HincII
HpaI MmeI MscI MspA1I NspI PasI PciI PflMI
PspOMI PvuII SspI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI
ClaI CspCI DraI EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI StyI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI