Restriction map for
pFN21AE5287
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# NO CUT ENZYMES ( 193 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BpuAI
BsaAI BsaBI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MslI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tsp509I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 322 GTCTAC GT^[AC][GT]AC
AspI 316 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 549 GGTGCC G^G[CT][GA]CC
BglII 626 AGATCT A^GATCT
BlpI 253 GCTCAGC GC^T[ACGT]AGC
BmyI 550 GTGCCC G[AGT]GC[ACT]^C
BpmI 436 CTCCAG CTGGAG(16/14)
Bpu1102I 253 GCTCAGC GC^T[ACGT]AGC
BsaI 49 GAGACC GGTCTC(1/5)
BsiYI 540 CCAGCAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 540 CCAGCAAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 49 GAGAC GTCTC(1/5)
Bsp1286I 550 GTGCCC G[AGT]GC[ACT]^C
BspMI 453 GCAGGT ACCTGC(4/8)
BsrI 655 CCAGT ACTGG(1/-1)
BstAPI 543 GCAAGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 626 AGATCT [GA]^GATC[CT]
CelII 253 GCTCAGC GC^T[ACGT]AGC
MaeIII 637 GTGAC ^GT[ACGT]AC
MflI 626 AGATCT [GA]^GATC[CT]
NlaIII 121 CATG CATG^
PflFI 316 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 540 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
TfiI 79 GAATC G^A[AT]TC
Tsp45I 637 GTGAC ^GT[GC]AC
Tth111I 316 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 540 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 626 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AluI 309 AGCT AG^CT
567 AGCT
AlwNI 184 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
496 CAGCCCCTG
BssKI 24 CCTGG ^CC[ACGT]GG
153 CCAGG
BstF5I 12 CATCC GGATG(2/0)
144 GGATG
BstNI 24 CCTGG CC^[AT]GG
153 CCAGG
Cac8I 19 GCCTGC GC[ACGT]^[ACGT]GC
258 GCCTGC
DraII 5 AGGTCCT [GA]G^G[ACGT]CC[CT]
455 AGGTCCC
EcoO109I 5 AGGTCCT [GA]G^G[ACGT]CC[CT]
455 AGGTCCC
EcoRII 24 CCTGG ^CC[AT]GG
153 CCAGG
FokI 12 CATCC GGATG(9/13)
144 GGATG
MboII 228 TCTTC GAAGA(8/7)
420 TCTTC
MvaI 24 CCTGG CC^[AT]GG
153 CCAGG
NlaIV 456 GGTCCC GG[ACGT]^[ACGT]CC
549 GGTGCC
PleI 477 GACTC GAGTC(4/5)
639 GACTC
PpuMI 5 AGGTCCT [GA]G^G[AT]CC[CT]
455 AGGTCCC
ScrFI 24 CCTGG CC^[ACGT]GG
153 CCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AvaII BbvCI BbvI BsmFI Cfr13I
DdeI DpnII Fnu4HI HinfI ItaI
MboI MnlI MseI MwoI NdeII
Sau3AI Sau96I SfaNI Tru9I TseI
TspRI