(Linear) (Six-base) MAPSORT of: KIEE5287.seq  Check: 3499  from: 1  to: 678



KIEE5287.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:00  ..

AccI GT'mk_AC

Cuts at:      0     323     678 
   Size:        323     355
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     358     678 
   Size:        358     320
 
AlwNI CAG_nnn'CTG

Cuts at:      0     189     501     678 
   Size:        189     312     177

  Fragments arranged by size: 

                 312     189     177
 
BanI G'GyrC_C

Cuts at:      0     549     678 
   Size:        549     129
 
BbvCI CC'TCA_GC

Cuts at:      0     281     461     494     515     678 
   Size:        281     180      33      21     163

  Fragments arranged by size: 

                 281     180     163      33      21
 
BglII A'GATC_T

Cuts at:      0     626     678 
   Size:        626      52
 
BlpI GC'TnA_GC

Cuts at:      0     254     678 
   Size:        254     424
 
Bme1580I G_kGCm'C

Cuts at:      0     554     678 
   Size:        554     124
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     649     678 
   Size:        649      29
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     678 
   Size:        421     257
 
Bpu10I CC'TnA_GC

Cuts at:      0     281     461     494     515     678 
   Size:        281     180      33      21     163

  Fragments arranged by size: 

                 281     180     163      33      21
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      43     678 
   Size:         43     635
 
BseYI C'CCAG_C

Cuts at:      0     539     678 
   Size:        539     139
 
Bsp1286I G_dGCh'C

Cuts at:      0     554     678 
   Size:        554     124
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     444     678 
   Size:        444     234
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     549     678 
   Size:        549     129
 
BstYI r'GATC_y

Cuts at:      0     626     678 
   Size:        626      52
 
BtsI GCAGTG_nn'

Cuts at:      0      78     678 
   Size:         78     600
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     421     678 
   Size:        421     257
 
EcoO109I rG'GnC_Cy

Cuts at:      0       6     456     678 
   Size:          6     450     222

  Fragments arranged by size: 

                 450     222       6
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     385     678 
   Size:        385     293
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     546     678 
   Size:        546     132
 
PpuMI rG'GwC_Cy

Cuts at:      0       6     456     678 
   Size:          6     450     222

  Fragments arranged by size: 

                 450     222       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     634     678 
   Size:        634      44
 
Tth111I GACn'n_nGTC

Cuts at:      0     319     678 
   Size:        319     359
 

 Enzymes that do cut:

     AccI     AlfI    AlwNI     BanI    BbvCI    BglII     BlpI Bme1580I
     BmrI     BpmI   Bpu10I     BsaI    BseYI Bsp1286I    BspMI   BstAPI
    BstYI     BtsI  Eco57MI EcoO109I     MmeI    PflMI    PpuMI    TaqII
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI     BmtI
     BplI    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI