Restriction map for
pFN21AE5162
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwI
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BpmI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
HphI KasI KpnI KspI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MseI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AciI 263 GCGG CCGC(-3/-1)
AluI 65 AGCT AG^CT
ApaI 29 GGGCCC GGGCC^C
AvrII 34 CCTAGG C^CTAGG
BglII 239 AGATCT A^GATCT
BlnI 34 CCTAGG C^CTAGG
BlpI 314 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 314 GCTCAGC GC^T[ACGT]AGC
BsaI 249 GGTCTC GGTCTC(1/5)
BseRI 46 GAGGAG GAGGAG(10/8)
BsmAI 250 GTCTC GTCTC(1/5)
BspMI 4 ACCTGC ACCTGC(4/8)
BsrDI 219 CATTGC GCAATG(2/0)
BsrI 72 ACTGG ACTGG(1/-1)
BstXI 302 CCACGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 239 AGATCT [GA]^GATC[CT]
Cac8I 66 GCTAGC GC[ACGT]^[ACGT]GC
CelII 314 GCTCAGC GC^T[ACGT]AGC
EarI 256 GAAGAG CTCTTC(1/4)
EcoNI 187 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 76 GACGC GACGC(5/10)
Ksp632I 256 GAAGAG CTCTTC(1/4)
MboII 256 GAAGA GAAGA(8/7)
MflI 239 AGATCT [GA]^GATC[CT]
MwoI 70 GCACTGGACGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 66 GCTAGC G^CTAGC
NlaIII 327 CATG CATG^
PleI 153 GACTC GAGTC(4/5)
PstI 90 CTGCAG CTGCA^G
SfaNI 8 GCATC GCATC(5/9)
SfcI 90 CTGCAG C^T[GA][CT]AG
StuI 37 AGGCCT AGG^CCT
TfiI 95 GAATC G^A[AT]TC
XhoII 239 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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BanII 29 GGGCCC G[GA]GC[CT]^C
312 GGGCTC
BbvI 79 GCAGC GCAGC(8/12)
89 GCTGC
BfaI 35 CTAG C^TAG
67 CTAG
BmyI 29 GGGCCC G[AGT]GC[ACT]^C
312 GGGCTC
BsiYI 184 CCTCCTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
187 CCTGGGGGAGG
BslI 184 CCTCCTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
187 CCTGGGGGAGG
Bsp1286I 29 GGGCCC G[AGT]GC[ACT]^C
312 GGGCTC
Cfr13I 29 GGGCC G^G[ACGT]CC
30 GGCCC
DpnII 54 GATC ^GATC
240 GATC
DraII 28 GGGGCCC [GA]G^G[ACGT]CC[CT]
29 GGGCCCC
EcoO109I 28 GGGGCCC [GA]G^G[ACGT]CC[CT]
29 GGGCCCC
EcoT14I 34 CCTAGG C^C[AT][AT]GG
320 CCTTGG
Fnu4HI 79 GCAGC GC^[ACGT]GC
89 GCTGC
HinfI 95 GAATC G^A[ACGT]TC
153 GACTC
ItaI 79 GCAGC GC^[ACGT]GC
89 GCTGC
MaeI 35 CTAG C^TAG
67 CTAG
MboI 54 GATC ^GATC
240 GATC
NdeII 54 GATC ^GATC
240 GATC
Sau3AI 54 GATC ^GATC
240 GATC
Sau96I 29 GGGCC G^G[ACGT]CC
30 GGCCC
StyI 34 CCTAGG C^C[AT][AT]GG
320 CCTTGG
TseI 79 GCAGC G^C[AT]GC
89 GCTGC
TspRI 71 CACTG CA[GC]TG[ACGT][ACGT]^
213 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 12 enzymes) #
#########################################
BsaJI BssKI BstF5I BstNI DdeI
EcoRII FokI HaeIII MnlI MvaI
NlaIV ScrFI