(Linear) (Six-base) MAPSORT of: KIEE5162.seq  Check: 2636  from: 1  to: 336



KIEE5162.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     336 
   Size:        245      91
 
ApaI G_GGCC'C

Cuts at:      0      33     336 
   Size:         33     303
 
AvrII C'CTAG_G

Cuts at:      0      34     336 
   Size:         34     302
 
BanII G_rGCy'C

Cuts at:      0      33     316     336 
   Size:         33     283      20

  Fragments arranged by size: 

                 283      33      20
 
BglII A'GATC_T

Cuts at:      0     239     336 
   Size:        239      97
 
BlpI GC'TnA_GC

Cuts at:      0     315     336 
   Size:        315      21
 
Bme1580I G_kGCm'C

Cuts at:      0      33     336 
   Size:         33     303
 
BmtI G_CTAG'C

Cuts at:      0      70     336 
   Size:         70     266
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     255     336 
   Size:        255      81
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      61     336 
   Size:         61     275
 
BseYI C'CCAG_C

Cuts at:      0     130     286     336 
   Size:        130     156      50

  Fragments arranged by size: 

                 156     130      50
 
Bsp1286I G_dGCh'C

Cuts at:      0      33     316     336 
   Size:         33     283      20

  Fragments arranged by size: 

                 283      33      20
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      13     336 
   Size:         13     323
 
BsrDI GCAATG_nn'

Cuts at:      0     218     336 
   Size:        218     118
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     309     336 
   Size:        309      27
 
BstYI r'GATC_y

Cuts at:      0     239     336 
   Size:        239      97
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     201     236     336 
   Size:        201      35     100

  Fragments arranged by size: 

                 201     100      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     251     336 
   Size:        251      85
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     336 
   Size:        245      91
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     191     336 
   Size:        191     145
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29      30     336 
   Size:         29       1     306

  Fragments arranged by size: 

                 306      29       1
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     320     336 
   Size:        320      16
 
NheI G'CTAG_C

Cuts at:      0      66     336 
   Size:         66     270
 
PasI CC'CwG_GG

Cuts at:      0      25      84     336 
   Size:         25      59     252

  Fragments arranged by size: 

                 252      59      25
 
PspOMI G'GGCC_C

Cuts at:      0      29     336 
   Size:         29     307
 
PstI C_TGCA'G

Cuts at:      0      94     336 
   Size:         94     242
 
SfcI C'TryA_G

Cuts at:      0      90     336 
   Size:         90     246
 
StuI AGG'CCT

Cuts at:      0      39     336 
   Size:         39     297
 
StyI C'CwwG_G

Cuts at:      0      34     320     336 
   Size:         34     286      16

  Fragments arranged by size: 

                 286      34      16
 

 Enzymes that do cut:

     AcuI     ApaI    AvrII    BanII    BglII     BlpI Bme1580I     BmtI
     BsaI    BseRI    BseYI Bsp1286I    BspMI    BsrDI    BstXI    BstYI
    CspCI     EarI  Eco57MI    EcoNI EcoO109I     MmeI     NheI     PasI
   PspOMI     PstI     SfcI     StuI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI