################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BseAI BseRI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HpaI KasI KpnI Ksp632I KspI MamI MflI MluI MluNI MroI MscI MslI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tsp45I Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AciI 477 CCGC CCGC(-3/-1) AclI 814 AACGTT AA^CGTT AfaI 574 GTAC GT^AC ApaI 20 GGGCCC GGGCC^C AseI 132 ATTAAT AT^TAAT AsnI 132 ATTAAT AT^TAAT AvaII 1137 GGACC G^G[AT]CC BbsI 659 GTCTTC GAAGAC(2/6) BcnI 1094 CCGGG CC^[GC]GG BlpI 103 GCTAAGC GC^T[ACGT]AGC BpmI 604 CTGGAG CTGGAG(16/14) Bpu1102I 103 GCTAAGC GC^T[ACGT]AGC BpuAI 659 GTCTTC GAAGAC(2/6) BsaI 316 GAGACC GGTCTC(1/5) BsaWI 878 TCCGGT [AT]^CCGG[AT] BsgI 716 GTGCAG GTGCAG(16/14) BsiEI 15 CGATCG CG[GA][CT]^CG BsiYI 460 CCACAGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 460 CCACAGAGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 316 GAGAC GTCTC(1/5) BsmFI 438 GTCCC GGGAC(10/14) BsrDI 97 GCAATG GCAATG(2/0) Cac8I 418 GCTCGC GC[ACGT]^[ACGT]GC CelII 103 GCTAAGC GC^T[ACGT]AGC DraIII 461 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT22I 566 ATGCAT ATGCA^T HincII 735 GTTGAC GT[CT]^[GA]AC HindII 735 GTTGAC GT[CT]^[GA]AC HphI 1102 GGTGA GGTGA(8/7) MaeII 815 ACGT A^CGT MfeI 481 CAATTG C^AATTG MspA1I 1004 CAGCTG C[AC]G^C[GT]G MunI 481 CAATTG C^AATTG NciI 1094 CCGGG CC^[GC]GG NsiI 566 ATGCAT ATGCA^T PflMI 460 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 132 ATTAAT AT^TAAT Psp1406I 814 AACGTT AA^CGTT PvuI 15 CGATCG CGAT^CG PvuII 1004 CAGCTG CAG^CTG RsaI 574 GTAC GT^AC TspRI 999 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 460 CCACAGAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 163 GAATTT [GA]^AATT[CT] 399 AAATTT ApoI 163 GAATTT [GA]^AATT[CT] 399 AAATTT Asp700I 241 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 793 GAAATTATTC AspHI 47 GAGCTC G[AT]GC[AT]^C 416 GAGCTC BfaI 56 CTAG C^TAG 823 CTAG BsaJI 110 CCCAGG C^C[ACGT][ACGT]GG 1094 CCGGGG BsiHKAI 47 GAGCTC G[AT]GC[AT]^C 416 GAGCTC BsmI 153 GAATGC GAATGC(1/-1) 675 GAATGC BsrI 890 ACTGG ACTGG(1/-1) 188 CCAGT DraII 20 GGGCCCC [GA]G^G[ACGT]CC[CT] 37 AGGCCCC EcoO109I 20 GGGCCCC [GA]G^G[ACGT]CC[CT] 37 AGGCCCC HapII 879 CCGG C^CGG 1094 CCGG HindIII 865 AAGCTT A^AGCTT 1154 AAGCTT HpaII 879 CCGG C^CGG 1094 CCGG MaeI 56 CTAG C^TAG 823 CTAG MspI 879 CCGG C^CGG 1094 CCGG NlaIII 565 CATG CATG^ 904 CATG PleI 91 GAGTC GAGTC(4/5) 899 GAGTC PstI 834 CTGCAG CTGCA^G 1001 CTGCAG SacI 47 GAGCTC GAGCT^C 416 GAGCTC SfcI 834 CTGCAG C^T[GA][CT]AG 1001 CTGCAG XmnI 241 GAATATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 793 GAAATTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AluI AlwI BanII BbvI BmyI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIV Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI Tsp509I TthHB8I