(Circular) (Six-base) MAPSORT of: pFN21AE5549.seq Check: 2710 from: 1 to: 5905 pFN21AE5549 5905 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 2820 4032 4405 1563 Size: 1257 1212 373 3063 Fragments arranged by size: 3063 1257 1212 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 2639 3968 5352 5620 1632 Size: 531 476 1329 1384 268 1917 Fragments arranged by size: 1917 1384 1329 531 476 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 4804 5781 1203 Size: 3601 977 1327 Fragments arranged by size: 3601 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 3648 5220 3648 Size: 1572 4333 ApaI G_GGCC'C Cuts at: 2029 2029 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2168 2404 3214 3440 3592 5701 1493 Size: 675 236 810 226 152 2109 1697 Fragments arranged by size: 2109 1697 810 675 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 2138 4459 160 Size: 872 1106 2321 1606 Fragments arranged by size: 2321 1606 1106 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3230 1870 Size: 73 1287 4545 Fragments arranged by size: 4545 1287 73 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3226 4681 618 Size: 325 217 627 1439 1455 1842 Fragments arranged by size: 1842 1455 1439 627 325 217 BanII G_rGCy'C Cuts at: 1391 2029 2056 2425 3224 1391 Size: 638 27 369 799 4072 Fragments arranged by size: 4072 799 638 369 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2657 928 Size: 971 758 4176 Fragments arranged by size: 4176 971 758 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4096 4130 1903 Size: 34 2159 34 3678 Fragments arranged by size: 3678 2159 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3797 5007 3797 Size: 1210 4695 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BfrBI ATG'CAT Cuts at: 2573 2573 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4516 136 Size: 107 122 71 631 605 2844 1525 Fragments arranged by size: 2844 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 2109 3322 2109 Size: 1213 4692 Bme1580I G_kGCm'C Cuts at: 1790 2029 3966 5122 1790 Size: 239 1937 1156 2573 Fragments arranged by size: 2573 1937 1156 239 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2905 3636 4594 Size: 416 53 83 399 24 1488 731 958 Cuts at: 4594 442 Size: 1753 Fragments arranged by size: 1753 1488 958 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2630 4565 1239 Size: 1391 1935 2579 Fragments arranged by size: 2579 1935 1391 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 4568 882 Size: 617 816 2253 2219 Fragments arranged by size: 2253 2219 816 617 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3712 4094 4729 275 Size: 53 83 186 936 2179 382 635 1451 Fragments arranged by size: 2179 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2883 4338 4736 5010 5157 5568 2883 Size: 1455 398 274 147 411 3220 Fragments arranged by size: 3220 1455 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5680 Fragments arranged by size: 5680 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5905 BseYI C'CCAG_C Cuts at: 5108 5604 5108 Size: 496 5409 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2742 1163 Size: 573 1006 4326 Fragments arranged by size: 4326 1006 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2023 3355 4116 5144 1540 Size: 275 186 22 1332 761 1028 2301 Fragments arranged by size: 2301 1332 1028 761 275 186 22 BsiHKAI G_wGCw'C Cuts at: 2056 2425 3966 4051 5122 2056 Size: 369 1541 85 1071 2839 Fragments arranged by size: 2839 1541 1071 369 85 BsmI GAATG_Cn' Cuts at: 2164 2686 3416 3509 2164 Size: 522 730 93 4560 Fragments arranged by size: 4560 730 522 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2056 2425 3224 3966 4051 5122 Size: 399 239 27 369 799 742 85 1071 Cuts at: 5122 1391 Size: 2174 Fragments arranged by size: 2174 1071 799 742 399 369 239 85 27 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3792 1394 Size: 429 1969 3507 Fragments arranged by size: 3507 1969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2109 4400 4574 65 Size: 1183 861 2291 174 1396 Fragments arranged by size: 2291 1396 1183 861 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4549 4736 1700 Size: 110 2739 187 2869 Fragments arranged by size: 2869 2739 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstYI r'GATC_y Cuts at: 1972 3235 3602 3987 4004 5456 5542 5554 5900 Size: 1263 367 385 17 1452 86 12 346 Cuts at: 5900 1972 Size: 1977 Fragments arranged by size: 1977 1452 1263 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5353 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 3003 3421 4215 4235 3003 Size: 418 794 20 4673 Fragments arranged by size: 4673 794 418 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DraIII CAC_nnn'GTG Cuts at: 2471 2471 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4912 809 Size: 507 3596 1802 Fragments arranged by size: 3596 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3352 4241 5870 Size: 54 1425 50 275 18 1522 889 1629 Cuts at: 5870 8 Size: 43 Fragments arranged by size: 1629 1522 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3835 1353 Size: 2482 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2471 4492 4866 5012 387 Size: 1064 138 882 2021 374 146 1280 Fragments arranged by size: 2021 1280 1064 882 374 146 138 EcoICRI GAG'CTC Cuts at: 2054 2423 2054 Size: 369 5536 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2163 2630 2639 3968 4565 5352 5620 Size: 393 531 467 9 1329 597 787 268 Cuts at: 5620 1239 Size: 1524 Fragments arranged by size: 1524 1329 787 597 531 467 393 268 9 EcoO109I rG'GnC_Cy Cuts at: 2026 2043 2026 Size: 17 5888 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3062 3062 Size: 5905 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 5052 1678 Size: 3374 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373 Size: 32 135 32 2974 32 42 32 2626 Fragments arranged by size: 2974 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2742 3249 3495 669 Size: 988 281 804 507 246 3079 Fragments arranged by size: 3079 988 804 507 281 246 HindIII A'AGCT_T Cuts at: 748 2870 3159 748 Size: 2122 289 3494 Fragments arranged by size: 3494 2122 289 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 2486 3504 2486 Size: 1018 4887 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1989 2486 5019 5203 1305 Size: 684 497 2533 184 2007 Fragments arranged by size: 2533 2007 684 497 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4083 Fragments arranged by size: 4083 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3863 4222 4381 518 Size: 648 2697 359 159 2042 Fragments arranged by size: 2697 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3011 3321 3998 5146 5391 1606 Size: 1405 310 677 1148 245 2120 Fragments arranged by size: 2120 1405 1148 677 310 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5353 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NsiI A_TGCA'T Cuts at: 2575 2575 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 3263 4808 1132 Size: 97 2034 1545 2229 Fragments arranged by size: 2229 2034 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 4804 4804 Size: 5905 PflMI CCAn_nnn'nTGG Cuts at: 1108 2471 1108 Size: 1363 4542 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2025 2025 Size: 5905 PstI C_TGCA'G Cuts at: 830 2843 3010 3257 830 Size: 2013 167 247 3478 Fragments arranged by size: 3478 2013 247 167 PvuI CG_AT'CG Cuts at: 2001 2023 2001 Size: 22 5883 PvuII CAG'CTG Cuts at: 3011 3011 Size: 5905 SacI G_AGCT'C Cuts at: 2056 2425 2056 Size: 369 5536 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2839 3006 3253 4388 5069 5260 826 Size: 219 1794 167 247 1135 681 191 1471 Fragments arranged by size: 1794 1471 1135 681 247 219 191 167 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 3344 513 Size: 552 2279 3074 Fragments arranged by size: 3074 2279 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3932 3949 4102 4287 4732 325 Size: 1255 2352 17 153 185 445 1498 Fragments arranged by size: 2352 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2578 4151 5863 Size: 274 80 33 51 1443 601 1573 1712 Cuts at: 5863 96 Size: 138 Fragments arranged by size: 1712 1573 1443 601 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 2250 2802 4034 2250 Size: 552 1232 4121 Fragments arranged by size: 4121 1232 552 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI