(Linear) (Six-base) MAPSORT of: KIEE5549.seq Check: 8207 from: 1 to: 1200 KIEE5549.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. AclI AA'CG_TT Cuts at: 0 815 1200 Size: 815 385 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 158 634 1200 Size: 158 476 566 Fragments arranged by size: 566 476 158 ApaI G_GGCC'C Cuts at: 0 24 1200 Size: 24 1176 ApoI r'AATT_y Cuts at: 0 163 399 1200 Size: 163 236 801 Fragments arranged by size: 801 236 163 AseI AT'TA_AT Cuts at: 0 133 1200 Size: 133 1067 BanII G_rGCy'C Cuts at: 0 24 51 420 1200 Size: 24 27 369 780 Fragments arranged by size: 780 369 27 24 BbsI GAAGACnn'nnnn_ Cuts at: 0 652 1200 Size: 652 548 BfrBI ATG'CAT Cuts at: 0 568 1200 Size: 568 632 BlpI GC'TnA_GC Cuts at: 0 104 1200 Size: 104 1096 Bme1580I G_kGCm'C Cuts at: 0 24 1200 Size: 24 1176 BmrI ACTGGGnnnn_n' Cuts at: 0 900 1200 Size: 900 300 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 625 1200 Size: 625 575 BsaI GGTCTCn'nnnn_ Cuts at: 0 310 1200 Size: 310 890 BsaWI w'CCGG_w Cuts at: 0 878 1200 Size: 878 322 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 737 1200 Size: 737 463 BsiEI CG_ry'CG Cuts at: 0 18 1200 Size: 18 1182 BsiHKAI G_wGCw'C Cuts at: 0 51 420 1200 Size: 51 369 780 Fragments arranged by size: 780 369 51 BsmI GAATG_Cn' Cuts at: 0 159 681 1200 Size: 159 522 519 Fragments arranged by size: 522 519 159 Bsp1286I G_dGCh'C Cuts at: 0 24 51 420 1200 Size: 24 27 369 780 Fragments arranged by size: 780 369 27 24 BsrDI GCAATG_nn' Cuts at: 0 104 1200 Size: 104 1096 BtsI GCAGTG_nn' Cuts at: 0 998 1200 Size: 998 202 DraIII CAC_nnn'GTG Cuts at: 0 466 1200 Size: 466 734 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 466 1200 Size: 466 734 EcoICRI GAG'CTC Cuts at: 0 49 418 1200 Size: 49 369 782 Fragments arranged by size: 782 369 49 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 158 625 634 1200 Size: 158 467 9 566 Fragments arranged by size: 566 467 158 9 EcoO109I rG'GnC_Cy Cuts at: 0 21 38 1200 Size: 21 17 1162 Fragments arranged by size: 1162 21 17 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1057 1200 Size: 1057 143 HincII GTy'rAC Cuts at: 0 737 1200 Size: 737 463 HindIII A'AGCT_T Cuts at: 0 865 1154 1200 Size: 865 289 46 Fragments arranged by size: 865 289 46 MfeI C'AATT_G Cuts at: 0 481 1200 Size: 481 719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 481 1200 Size: 481 719 MspA1I CmG'CkG Cuts at: 0 1006 1200 Size: 1006 194 NsiI A_TGCA'T Cuts at: 0 570 1200 Size: 570 630 PflMI CCAn_nnn'nTGG Cuts at: 0 466 1200 Size: 466 734 PspOMI G'GGCC_C Cuts at: 0 20 1200 Size: 20 1180 PstI C_TGCA'G Cuts at: 0 838 1005 1200 Size: 838 167 195 Fragments arranged by size: 838 195 167 PvuI CG_AT'CG Cuts at: 0 18 1200 Size: 18 1182 PvuII CAG'CTG Cuts at: 0 1006 1200 Size: 1006 194 SacI G_AGCT'C Cuts at: 0 51 420 1200 Size: 51 369 780 Fragments arranged by size: 780 369 51 SfcI C'TryA_G Cuts at: 0 834 1001 1200 Size: 834 167 199 Fragments arranged by size: 834 199 167 TatI w'GTAC_w Cuts at: 0 573 1200 Size: 573 627 XmnI GAAnn'nnTTC Cuts at: 0 245 797 1200 Size: 245 552 403 Fragments arranged by size: 552 403 245 Enzymes that do cut: AclI AcuI ApaI ApoI AseI BanII BbsI BfrBI BlpI Bme1580I BmrI BpmI BsaI BsaWI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI BtsI DraIII EciI EcoICRI Eco57MI EcoO109I FalI HincII HindIII MfeI MmeI MspA1I NsiI PflMI PspOMI PstI PvuI PvuII SacI SfcI TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EaeI EagI EarI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI