(Circular) (Six-base) MAPSORT of: pFN21AE2564.seq Check: 7048 from: 1 to: 6529 pFN21AE2564 6529 bp With 182 enzymes: SgfI * March 13, 2015 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3107 3107 Size: 6529 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4339 278 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3872 1937 Size: 1935 4594 Acc65I G'GTAC_C Cuts at: 3850 3850 Size: 6529 AclI AA'CG_TT Cuts at: 1563 4656 5029 1563 Size: 3093 373 3063 Fragments arranged by size: 3093 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2558 3328 3751 4592 5976 6244 1632 Size: 926 770 423 841 1384 268 1917 Fragments arranged by size: 1917 1384 926 841 770 423 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6332 AflIII A'CryG_T Cuts at: 1203 5428 6405 1203 Size: 4225 977 1327 Fragments arranged by size: 4225 1327 977 AgeI A'CCGG_T Cuts at: 5360 5360 Size: 6529 AhdI GACnn_n'nnGTC Cuts at: 5258 5258 Size: 6529 AleI CACnn'nnGTG Cuts at: 1166 2682 3452 3617 3756 1166 Size: 1516 770 165 139 3939 Fragments arranged by size: 3939 1516 770 165 139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6529 AlwNI CAG_nnn'CTG Cuts at: 2022 2403 3053 4272 5844 2022 Size: 381 650 1219 1572 2707 Fragments arranged by size: 2707 1572 1219 650 381 ApaI G_GGCC'C Cuts at: 2102 2886 2102 Size: 784 5745 ApaLI G'TGCA_C Cuts at: 3740 4586 5742 3740 Size: 846 1156 4527 Fragments arranged by size: 4527 1156 846 ApoI r'AATT_y Cuts at: 1493 3838 4064 4216 6325 1493 Size: 2345 226 152 2109 1697 Fragments arranged by size: 2345 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5083 160 Size: 872 4051 1606 Fragments arranged by size: 4051 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 AvaI C'yCGr_G Cuts at: 1870 1943 2382 2628 3854 1870 Size: 73 439 246 1226 4545 Fragments arranged by size: 4545 1226 439 246 73 BamHI G'GATC_C Cuts at: 2817 3859 4226 2817 Size: 1042 367 5120 Fragments arranged by size: 5120 1042 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2204 2504 3086 3360 3446 Size: 325 217 627 417 300 582 274 86 Cuts at: 3446 3850 5305 618 Size: 404 1455 1842 Fragments arranged by size: 1842 1455 627 582 417 404 325 300 274 217 86 BanII G_rGCy'C Cuts at: 1391 2036 2102 2712 2886 3848 1391 Size: 645 66 610 174 962 4072 Fragments arranged by size: 4072 962 645 610 174 66 BbeI G_GCGC'C Cuts at: 2508 3364 2508 Size: 856 5673 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3210 3495 928 Size: 971 1311 285 3962 Fragments arranged by size: 3962 1311 971 285 BbvCI CC'TCA_GC Cuts at: 2748 3459 2748 Size: 711 5818 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2402 2436 4720 4754 1903 Size: 34 50 34 381 34 2284 34 3678 Fragments arranged by size: 3678 2284 381 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4421 5631 4421 Size: 1210 5319 BclI T'GATC_A Cuts at: 1548 1548 Size: 6529 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2454 2939 5140 Size: 107 122 71 631 605 782 485 2201 Cuts at: 5140 136 Size: 1525 Fragments arranged by size: 2201 1525 782 631 605 485 122 107 71 BglII A'GATC_T Cuts at: 3157 6524 3157 Size: 3367 3162 BlpI GC'TnA_GC Cuts at: 3259 3268 3946 3259 Size: 9 678 5842 Fragments arranged by size: 5842 678 9 Bme1580I G_kGCm'C Cuts at: 1790 2102 2207 2558 2729 2886 3079 3744 4590 Size: 312 105 351 171 157 193 665 846 Cuts at: 4590 5746 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 846 665 351 312 193 171 157 105 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6529 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2811 2926 2963 Size: 416 53 83 399 24 1394 115 37 Cuts at: 2963 3007 4260 5218 442 Size: 44 1253 958 1753 Fragments arranged by size: 1753 1394 1253 958 416 399 115 83 53 44 37 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6529 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2664 3529 2664 Size: 865 5664 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2535 2948 3150 3626 5189 1239 Size: 1296 413 202 476 1563 2579 Fragments arranged by size: 2579 1563 1296 476 413 202 Bpu10I CC'TnA_GC Cuts at: 2748 3459 2748 Size: 711 5818 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2359 4654 5519 5817 2359 Size: 2295 865 298 3071 Fragments arranged by size: 3071 2295 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2085 2804 2981 3182 5192 882 Size: 617 586 719 177 201 2010 2219 Fragments arranged by size: 2219 2010 719 617 586 201 177 BsaAI yAC'GTr Cuts at: 493 1204 4320 493 Size: 711 3116 2702 Fragments arranged by size: 3116 2702 711 BsaBI GATnn'nnATC Cuts at: 3608 6523 3608 Size: 2915 3614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2505 3295 3361 4336 Size: 53 83 186 936 972 790 66 975 Cuts at: 4336 4718 5353 275 Size: 382 635 1451 Fragments arranged by size: 1451 975 972 936 790 635 382 186 83 66 53 BsaWI w'CCGG_w Cuts at: 2781 4962 5360 5634 5781 6192 2781 Size: 2181 398 274 147 411 3118 Fragments arranged by size: 3118 2181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2978 3008 1178 Size: 30 165 30 1575 30 4699 Fragments arranged by size: 4699 1575 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3301 1186 Size: 2115 4414 BseYI C'CCAG_C Cuts at: 2260 2388 2423 2611 2892 3356 3567 5732 6228 Size: 128 35 188 281 464 211 2165 496 Cuts at: 6228 2260 Size: 2561 Fragments arranged by size: 2561 2165 496 464 281 211 188 128 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2083 3028 3152 3203 1163 Size: 573 347 945 124 51 4489 Fragments arranged by size: 4489 945 573 347 124 51 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3979 4740 5768 1540 Size: 275 186 1978 761 1028 2301 Fragments arranged by size: 2301 1978 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2629 2712 3744 4590 4675 5746 2629 Size: 83 1032 846 85 1071 3412 Fragments arranged by size: 3412 1071 1032 846 85 83 BsmI GAATG_Cn' Cuts at: 4040 4133 4040 Size: 93 6436 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2102 2207 2558 2629 2712 2729 Size: 399 246 66 105 351 71 83 17 Cuts at: 2729 2886 3079 3744 3848 4590 4675 5746 1391 Size: 157 193 665 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 665 399 351 246 193 157 105 104 85 83 71 66 17 BspEI T'CCGG_A Cuts at: 6192 6192 Size: 6529 BspHI T'CATG_A Cuts at: 4418 6148 4418 Size: 1730 4799 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2589 3107 3884 844 Size: 1745 518 777 3489 Fragments arranged by size: 3489 1745 777 518 BsrBI CCG'CTC Cuts at: 1394 1823 3267 4416 1394 Size: 429 1444 1149 3507 Fragments arranged by size: 3507 1444 1149 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3705 5024 5198 65 Size: 1183 2457 1319 174 1396 Fragments arranged by size: 2457 1396 1319 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3207 3291 3579 5173 5360 1700 Size: 110 1397 84 288 1594 187 2869 Fragments arranged by size: 2869 1594 1397 288 187 110 84 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6529 BssSI C'ACGA_G Cuts at: 3380 4589 5601 3380 Size: 1209 1012 4308 Fragments arranged by size: 4308 1209 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2070 2211 794 Size: 1276 141 5112 Fragments arranged by size: 5112 1276 141 BstBI TT'CG_AA Cuts at: 5333 5333 Size: 6529 BstEII G'GTnAC_C Cuts at: 1184 3287 1184 Size: 2103 4426 BstYI r'GATC_y Cuts at: 1972 2817 3157 3318 3571 3859 4226 4611 4628 Size: 845 340 161 253 288 367 385 17 Cuts at: 4628 6080 6166 6178 6524 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 845 385 367 346 340 288 253 161 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2094 2934 2974 3388 513 Size: 552 1029 840 40 414 3654 Fragments arranged by size: 3654 1029 840 552 414 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2229 487 Size: 683 56 1003 4787 Fragments arranged by size: 4787 1003 683 56 BtsI GCAGTG_nn' Cuts at: 2033 2353 3123 4045 4839 4859 2033 Size: 320 770 922 794 20 3703 Fragments arranged by size: 3703 922 794 770 320 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6529 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6494 DraI TTT'AAA Cuts at: 3833 3833 Size: 6529 DraIII CAC_nnn'GTG Cuts at: 3498 3498 Size: 6529 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2471 3058 5536 809 Size: 507 1155 587 2478 1802 Fragments arranged by size: 2478 1802 1155 587 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3252 3805 3976 Size: 54 1425 50 275 18 1422 553 171 Cuts at: 3976 4865 6494 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1422 889 553 275 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3976 1537 Size: 275 2164 4090 Fragments arranged by size: 4090 2164 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3234 3768 4459 1353 Size: 1881 534 691 3423 Fragments arranged by size: 3423 1881 691 534 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5116 5490 5636 387 Size: 1064 138 3527 374 146 1280 Fragments arranged by size: 3527 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2710 2710 Size: 6529 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2535 2558 2948 3150 3328 3626 3751 Size: 393 903 23 390 202 178 298 125 Cuts at: 3751 4592 5189 5976 6244 1239 Size: 841 597 787 268 1524 Fragments arranged by size: 1524 903 841 787 597 393 390 298 268 202 178 125 23 EcoNI CCTnn'n_nnAGG Cuts at: 2313 3103 2313 Size: 790 5739 EcoO109I rG'GnC_Cy Cuts at: 2099 2578 2876 2883 2896 2922 3114 2099 Size: 479 298 7 13 26 192 5514 Fragments arranged by size: 5514 479 298 192 26 13 7 EcoRI G'AATT_C Cuts at: 3838 4216 3838 Size: 378 6151 EcoRV GAT'ATC Cuts at: 3430 3430 Size: 6529 FspI TGC'GCA Cuts at: 5035 5035 Size: 6529 HaeII r_GCGC'y Cuts at: 1678 2449 2508 2649 3364 5676 1678 Size: 771 59 141 715 2312 2531 Fragments arranged by size: 2531 2312 771 715 141 59 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2694 2726 3137 3169 3717 Size: 32 135 32 1122 32 411 32 548 Cuts at: 3717 3749 5170 5202 5244 5276 1373 Size: 32 1421 32 42 32 2626 Fragments arranged by size: 2626 1421 1122 548 411 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3873 4119 669 Size: 988 281 1935 246 3079 Fragments arranged by size: 3079 1935 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6529 HpaI GTT'AAC Cuts at: 4119 4119 Size: 6529 KasI G'GCGC_C Cuts at: 2504 3360 2504 Size: 856 5673 KpnI G_GTAC'C Cuts at: 3854 3854 Size: 6529 MfeI C'AATT_G Cuts at: 4128 4128 Size: 6529 MluI A'CGCG_T Cuts at: 6405 6405 Size: 6529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2845 5643 5827 1305 Size: 1540 2798 184 2007 Fragments arranged by size: 2798 2007 1540 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3254 3807 10 Size: 54 1425 343 1422 553 2732 Fragments arranged by size: 2732 1425 1422 553 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2682 3452 3557 3617 3756 4487 4846 Size: 648 1516 770 105 60 139 731 359 Cuts at: 4846 5005 518 Size: 159 2042 Fragments arranged by size: 2042 1516 770 731 648 359 159 139 105 60 MspA1I CmG'CkG Cuts at: 1606 2747 3945 4622 5770 6015 1606 Size: 1141 1198 677 1148 245 2120 Fragments arranged by size: 2120 1198 1148 1141 677 245 NarI GG'CG_CC Cuts at: 2505 3361 2505 Size: 856 5673 NcoI C'CATG_G Cuts at: 513 1065 2934 3388 513 Size: 552 1869 454 3654 Fragments arranged by size: 3654 1869 552 454 NdeI CA'TA_TG Cuts at: 387 3219 387 Size: 2832 3697 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6529 NotI GC'GGCC_GC Cuts at: 3976 3976 Size: 6529 NspI r_CATG'y Cuts at: 1132 1229 2588 3887 5432 1132 Size: 97 1359 1299 1545 2229 Fragments arranged by size: 2229 1545 1359 1299 97 PasI CC'CwG_GG Cuts at: 1348 2037 1348 Size: 689 5840 PciI A'CATG_T Cuts at: 5428 5428 Size: 6529 PflMI CCAn_nnn'nTGG Cuts at: 1108 2283 2550 3328 3406 1108 Size: 1175 267 778 78 4231 Fragments arranged by size: 4231 1175 778 267 78 PmeI GTTT'AAAC Cuts at: 3833 3833 Size: 6529 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4598 4630 6111 6143 4598 Size: 32 1481 32 4984 Fragments arranged by size: 4984 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2578 2876 2922 3114 2578 Size: 298 46 192 5993 Fragments arranged by size: 5993 298 192 46 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6529 PsiI TTA'TAA Cuts at: 4099 4099 Size: 6529 PspOMI G'GGCC_C Cuts at: 2098 2882 2098 Size: 784 5745 PstI C_TGCA'G Cuts at: 830 2235 2348 2985 3881 830 Size: 1405 113 637 896 3478 Fragments arranged by size: 3478 1405 896 637 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6529 PvuII CAG'CTG Cuts at: 2747 2747 Size: 6529 SacI G_AGCT'C Cuts at: 2712 2712 Size: 6529 SalI G'TCGA_C Cuts at: 1936 3871 1936 Size: 1935 4594 SanDI GG'GwC_CC Cuts at: 2922 3114 2922 Size: 192 6337 SbfI CC_TGCA'GG Cuts at: 2985 3881 2985 Size: 896 5633 ScaI AGT'ACT Cuts at: 4777 6489 4777 Size: 1712 4817 SexAI A'CCwGG_T Cuts at: 2990 2990 Size: 6529 SfcI C'TryA_G Cuts at: 826 1045 2231 2344 2944 2981 3877 5012 5693 Size: 219 1186 113 600 37 896 1135 681 Cuts at: 5693 5884 826 Size: 191 1471 Fragments arranged by size: 1471 1186 1135 896 681 600 219 191 113 37 SfiI GGCCn_nnn'nGGCC Cuts at: 2939 2939 Size: 6529 SfoI GGC'GCC Cuts at: 2506 3362 2506 Size: 856 5673 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6529 SgrAI Cr'CCGG_yG Cuts at: 1700 3291 1700 Size: 1591 4938 SmaI CCC'GGG Cuts at: 1872 2384 3856 1872 Size: 512 1472 4545 Fragments arranged by size: 4545 1472 512 SmlI C'TyrA_G Cuts at: 820 1017 1943 2338 2628 4633 5534 5796 820 Size: 197 926 395 290 2005 901 262 1553 Fragments arranged by size: 2005 1553 926 901 395 290 262 197 SnaBI TAC'GTA Cuts at: 493 4320 493 Size: 3827 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6529 SphI G_CATG'C Cuts at: 1132 2588 3887 1132 Size: 1456 1299 3774 Fragments arranged by size: 3774 1456 1299 SspI AAT'ATT Cuts at: 5 52 4453 5 Size: 47 4401 2081 Fragments arranged by size: 4401 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6529 StyI C'CwwG_G Cuts at: 513 1065 2934 3351 3388 3450 3968 513 Size: 552 1869 417 37 62 518 3074 Fragments arranged by size: 3074 1869 552 518 417 62 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2369 2657 3104 3361 3727 4556 4573 Size: 1255 789 288 447 257 366 829 17 Cuts at: 4573 4726 4911 5356 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 829 789 447 445 366 288 257 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3225 4775 6487 Size: 274 80 33 51 1443 1248 1550 1712 Cuts at: 6487 96 Size: 138 Fragments arranged by size: 1712 1550 1443 1248 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2278 2310 6489 6521 2278 Size: 32 4179 32 2286 Fragments arranged by size: 4179 2286 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6529 XbaI T'CTAG_A Cuts at: 3865 3865 Size: 6529 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2450 2450 Size: 6529 XhoI C'TCGA_G Cuts at: 1943 2628 1943 Size: 685 5844 XmaI C'CCGG_G Cuts at: 1870 2382 3854 1870 Size: 512 1472 4545 Fragments arranged by size: 4545 1472 512 XmnI GAAnn'nnTTC Cuts at: 4658 4658 Size: 6529 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4337 276 Size: 53 83 186 936 2803 2468 Fragments arranged by size: 2803 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SapI SrfI SwaI