##################################
# NO CUT ENZYMES (  194 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmAI       
BsmBI       BsmFI       BsmI        BsoBI       Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     FauI        FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
MwoI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NlaIV       NotI        
NruI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau96I      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tsp509I     Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AfaI          274  GTAC                 GT^AC
BbrPI         174  CACGTG               CAC^GTG
BbsI          112  GAAGAC               GAAGAC(2/6)
BbvI          142  GCAGC                GCAGC(8/12)
BcnI           82  CCCGG                CC^[GC]GG
BmyI           70  GTGCCC               G[AGT]GC[ACT]^C
BpuAI         112  GAAGAC               GAAGAC(2/6)
BsiYI         296  CCACGGACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          296  CCACGGACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I       70  GTGCCC               G[AGT]GC[ACT]^C
BsrDI         159  CATTGC               GCAATG(2/0)
BstNI         146  CCTGG                CC^[AT]GG
Cac8I         232  GCAAGC               GC[ACGT]^[ACGT]GC
DraI          264  TTTAAA               TTT^AAA
DsaI          296  CCACGG               C^C[GA][CT]GG
EarI           96  GAAGAG               CTCTTC(1/4)
EcoRII        146  CCTGG                ^CC[AT]GG
EcoT22I        26  ATGCAT               ATGCA^T
Fnu4HI        142  GCAGC                GC^[ACGT]GC
HapII          83  CCGG                 C^CGG
HpaII          83  CCGG                 C^CGG
HphI          370  GGTGA                GGTGA(8/7)
ItaI          142  GCAGC                GC^[ACGT]GC
Ksp632I        96  GAAGAG               CTCTTC(1/4)
MseI          265  TTAA                 T^TAA
MspA1I        182  CAGCTG               C[AC]G^C[GT]G
MspI           83  CCGG                 C^CGG
MvaI          146  CCTGG                CC^[AT]GG
NciI           82  CCCGG                CC^[GC]GG
NsiI           26  ATGCAT               ATGCA^T
PflMI         296  CCACGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         174  CACGTG               CAC^GTG
PmlI          174  CACGTG               CAC^GTG
PstI          140  CTGCAG               CTGCA^G
PvuII         182  CAGCTG               CAG^CTG
RsaI          274  GTAC                 GT^AC
SfcI          140  CTGCAG               C^T[GA][CT]AG
SmlI          411  CTTGAG               C^T[CT][GA]AG
Tru9I         265  TTAA                 T^TAA
TseI          142  GCAGC                G^C[AT]GC
TspRI          12  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        296  CCACGGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

BsaAI         174  CACGTG               [CT]AC^GT[GA]
              275  TACGTG              
BsaJI          81  CCCCGG               C^C[ACGT][ACGT]GG
              296  CCACGG              
BsrI          270  ACTGG                ACTGG(1/-1)
              302  ACTGG               
BssKI          82  CCCGG                ^CC[ACGT]GG
              146  CCTGG               
DdeI            5  CTGAG                C^T[ACGT]AG
              255  CTCAG               
MslI           39  CACAACTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              192  CATCGTTATG          
ScrFI          82  CCCGG                CC^[ACGT]GG
              146  CCTGG               
SfaNI         280  GATGC                GCATC(5/9)
               28  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   14 enzymes) #
#########################################

AluI        AlwI        BstYI       DpnII       MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
NdeII       Sau3AI      Tsp45I      XhoII