(Linear) (Six-base) MAPSORT of: KIEE6562.seq  Check: 7969  from: 1  to: 501



KIEE6562.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:33  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     115     368     501 
   Size:         38      77     253     133

  Fragments arranged by size: 

                 253     133      77      38
 
AleI CACnn'nnGTG

Cuts at:      0      43     501 
   Size:         43     458
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     119     501 
   Size:        119     382
 
BfrBI ATG'CAT

Cuts at:      0      28     501 
   Size:         28     473
 
Bme1580I G_kGCm'C

Cuts at:      0      74     501 
   Size:         74     427
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     432     501 
   Size:        432      69
 
BsaAI yAC'GTr

Cuts at:      0     176     277     501 
   Size:        176     101     224

  Fragments arranged by size: 

                 224     176     101
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     501 
   Size:         74     427
 
BsrDI GCAATG_nn'

Cuts at:      0     158     501 
   Size:        158     343
 
BstYI r'GATC_y

Cuts at:      0      64     258     305     501 
   Size:         64     194      47     196

  Fragments arranged by size: 

                 196     194      64      47
 
BtgI C'CryG_G

Cuts at:      0     296     501 
   Size:        296     205
 
BtsI GCAGTG_nn'

Cuts at:      0      11     501 
   Size:         11     490
 
DraI TTT'AAA

Cuts at:      0     266     501 
   Size:        266     235
 
EarI CTCTTCn'nnn_

Cuts at:      0      91     501 
   Size:         91     410
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     115     368     501 
   Size:         38      77     253     133

  Fragments arranged by size: 

                 253     133      77      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     325     357     501 
   Size:        325      32     144

  Fragments arranged by size: 

                 325     144      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     468     501 
   Size:        468      33
 
MslI CAynn'nnrTG

Cuts at:      0      43     196     501 
   Size:         43     153     305

  Fragments arranged by size: 

                 305     153      43
 
MspA1I CmG'CkG

Cuts at:      0     184     501 
   Size:        184     317
 
NsiI A_TGCA'T

Cuts at:      0      30     501 
   Size:         30     471
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     302     501 
   Size:        302     199
 
PmlI CAC'GTG

Cuts at:      0     176     501 
   Size:        176     325
 
PstI C_TGCA'G

Cuts at:      0     144     501 
   Size:        144     357
 
PvuII CAG'CTG

Cuts at:      0     184     501 
   Size:        184     317
 
SfcI C'TryA_G

Cuts at:      0     140     501 
   Size:        140     361
 
SmlI C'TyrA_G

Cuts at:      0     411     501 
   Size:        411      90
 

 Enzymes that do cut:

     AcuI     AleI     BbsI    BfrBI Bme1580I    BpuEI    BsaAI Bsp1286I
    BsrDI    BstYI     BtgI     BtsI     DraI     EarI  Eco57MI    Hin4I
     MmeI     MslI   MspA1I     NsiI    PflMI     PmlI     PstI    PvuII
     SfcI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI