################################## # NO CUT ENZYMES ( 204 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV FauI FbaI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MaeI MaeII MluI MluNI MroI MscI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AluI 29 AGCT AG^CT BbsI 35 GAAGAC GAAGAC(2/6) BbvI 292 GCTGC GCAGC(8/12) BciVI 448 GTATCC GTATCC(6/5) BpmI 352 CTCCAG CTGGAG(16/14) BpuAI 35 GAAGAC GAAGAC(2/6) BsaBI 135 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 337 GGTCTC GGTCTC(1/5) BsmI 243 GCATTC GAATGC(1/-1) BsrDI 271 CATTGC GCAATG(2/0) BstYI 235 GGATCT [GA]^GATC[CT] Cac8I 42 GCAAGC GC[ACGT]^[ACGT]GC DraI 510 TTTAAA TTT^AAA DsaI 88 CCACGG C^C[GA][CT]GG EcoT14I 282 CCAAGG C^C[AT][AT]GG EcoT22I 268 ATGCAT ATGCA^T Fnu4HI 292 GCTGC GC^[ACGT]GC HinfI 391 GAATC G^A[ACGT]TC HphI 522 GGTGA GGTGA(8/7) ItaI 292 GCTGC GC^[ACGT]GC MamI 135 GATTTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 149 CAATTG C^AATTG MflI 235 GGATCT [GA]^GATC[CT] MslI 534 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 149 CAATTG C^AATTG NlaIII 76 CATG CATG^ NsiI 268 ATGCAT ATGCA^T NspI 75 GCATGT [GA]CATG^[CT] SfaNI 267 GATGC GCATC(5/9) SspI 66 AATATT AAT^ATT StyI 282 CCAAGG C^C[AT][AT]GG TfiI 391 GAATC G^A[AT]TC TseI 292 GCTGC G^C[AT]GC XhoII 235 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AcsI 298 GAATTT [GA]^AATT[CT] 306 AAATTC AlwI 439 GATCC GGATC(4/5) 235 GGATC ApoI 298 GAATTT [GA]^AATT[CT] 306 AAATTC BsaJI 88 CCACGG C^C[ACGT][ACGT]GG 282 CCAAGG BseRI 10 CTCCTC GAGGAG(10/8) 19 CTCCTC BsmAI 252 GTCTC GTCTC(1/5) 338 GTCTC DpnII 236 GATC ^GATC 439 GATC MboI 236 GATC ^GATC 439 GATC MseI 98 TTAA T^TAA 511 TTAA NdeII 236 GATC ^GATC 439 GATC Sau3AI 236 GATC ^GATC 439 GATC Tru9I 98 TTAA T^TAA 511 TTAA TspRI 24 CACTG CA[GC]TG[ACGT][ACGT]^ 111 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### BsrI DdeI MaeIII MboII MnlI Tsp45I Tsp509I