################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmFI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT14I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MaeII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI TseI TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 116 GTAGAC GT^[AC][GT]AC AluI 135 AGCT AG^CT AlwI 562 GGATC GGATC(4/5) AlwNI 131 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 551 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 632 GTGCTC G[AT]GC[AT]^C BbsI 356 GTCTTC GAAGAC(2/6) BglI 341 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 632 GTGCTC G[AGT]GC[ACT]^C BpuAI 356 GTCTTC GAAGAC(2/6) BsaI 401 GGTCTC GGTCTC(1/5) BsiHKAI 632 GTGCTC G[AT]GC[AT]^C BsiYI 346 CCTGGCTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 346 CCTGGCTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 625 GCATTC GAATGC(1/-1) Bsp1286I 632 GTGCTC G[AGT]GC[ACT]^C BspHI 102 TCATGA T^CATGA BsrDI 211 CATTGC GCAATG(2/0) BstAPI 625 GCATTCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 230 GGTCACC G^GT[ACGT]ACC BstPI 230 GGTCACC G^GT[ACGT]ACC Cac8I 337 GCCTGC GC[ACGT]^[ACGT]GC CfoI 367 GCGC GCG^C DpnII 563 GATC ^GATC DraI 498 TTTAAA TTT^AAA EcoO65I 230 GGTCACC G^GT[ACGT]ACC EcoT22I 417 ATGCAT ATGCA^T HhaI 367 GCGC GCG^C HinP1I 367 GCGC G^CGC HphI 232 TCACC GGTGA(8/7) MboI 563 GATC ^GATC NdeII 563 GATC ^GATC NsiI 417 ATGCAT ATGCA^T PleI 180 GACTC GAGTC(4/5) RcaI 102 TCATGA T^CATGA Sau3AI 563 GATC ^GATC SspI 146 AATATT AAT^ATT Tsp45I 231 GTCAC ^GT[GC]AC XmnI 551 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcsI 552 AAATTC [GA]^AATT[CT] 687 GAATTT ApoI 552 AAATTC [GA]^AATT[CT] 687 GAATTT Bsp1407I 312 TGTACA T^GTACA 652 TGTACA BsrGI 312 TGTACA T^GTACA 652 TGTACA BsrI 197 ACTGG ACTGG(1/-1) 130 CCAGT BssKI 346 CCTGG ^CC[ACGT]GG 529 CCAGG BstNI 346 CCTGG CC^[AT]GG 529 CCAGG DdeI 250 CTCAG C^T[ACGT]AG 641 CTCAG EcoRII 346 CCTGG ^CC[AT]GG 529 CCAGG MnlI 485 CCTC CCTC(7/6) 92 GAGG MvaI 346 CCTGG CC^[AT]GG 529 CCAGG ScrFI 346 CCTGG CC^[ACGT]GG 529 CCAGG SfaNI 416 GATGC GCATC(5/9) 419 GCATC SfcI 137 CTGTAG C^T[GA][CT]AG 326 CTACAG SspBI 312 TGTACA T^GTACA 652 TGTACA TfiI 467 GATTC G^A[AT]TC 478 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 16 enzymes) # ######################################### AfaI AflIII BsmAI BspLU11I BstF5I FokI HinfI MaeIII MboII MseI MwoI NlaIII NspI RsaI Tru9I Tsp509I