################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcyI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssKI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT22I FauI Fnu4HI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HindIII HpaI ItaI KasI KpnI Ksp632I KspI MaeI MaeII MfeI MluI MluNI MscI MslI MspA1I MunI MvaI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TseI Tsp45I TspRI TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 109 TCCGGA T^CCGGA AfaI 337 GTAC GT^AC AflII 550 CTTAAG C^TTAAG AluI 475 AGCT AG^CT AlwI 204 GGATC GGATC(4/5) AlwNI 293 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 368 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 73 GGACC G^G[AT]CC BbsI 139 GAAGAC GAAGAC(2/6) BclI 234 TGATCA T^GATCA BfrI 550 CTTAAG C^TTAAG BpuAI 139 GAAGAC GAAGAC(2/6) BsaBI 229 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 366 GAGACC GGTCTC(1/5) BsaJI 332 CCATGG C^C[ACGT][ACGT]GG BsaWI 109 TCCGGA [AT]^CCGG[AT] BseAI 109 TCCGGA T^CCGGA BsmAI 366 GAGAC GTCTC(1/5) BspEI 109 TCCGGA T^CCGGA BssSI 185 CACGAG CACGAG(-5/-1) Cfr13I 73 GGACC G^G[ACGT]CC DsaI 332 CCATGG C^C[GA][CT]GG EcoRI 172 GAATTC G^AATTC EcoRV 216 GATATC GAT^ATC EcoT14I 332 CCATGG C^C[AT][AT]GG FbaI 234 TGATCA T^GATCA HapII 110 CCGG C^CGG HincII 285 GTTGAC GT[CT]^[GA]AC HindII 285 GTTGAC GT[CT]^[GA]AC HpaII 110 CCGG C^CGG HphI 355 GGTGA GGTGA(8/7) MaeIII 89 GTTAC ^GT[ACGT]AC MamI 229 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MnlI 427 GAGG CCTC(7/6) MroI 109 TCCGGA T^CCGGA MspI 110 CCGG C^CGG MwoI 532 GCACAACAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 332 CCATGG C^CATGG PflFI 368 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC RsaI 337 GTAC GT^AC Sau96I 73 GGACC G^G[ACGT]CC SfaNI 529 GATGC GCATC(5/9) SmlI 550 CTTAAG C^T[CT][GA]AG StyI 332 CCATGG C^C[AT][AT]GG Tth111I 368 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AcsI 172 GAATTC [GA]^AATT[CT] 192 AAATTT ApoI 172 GAATTC [GA]^AATT[CT] 192 AAATTT BglII 81 AGATCT A^GATCT 322 AGATCT BsrI 500 ACTGG ACTGG(1/-1) 516 CCAGT BstF5I 243 CATCC GGATG(2/0) 62 GGATG DdeI 85 CTGAG C^T[ACGT]AG 397 CTTAG FokI 243 CATCC GGATG(9/13) 62 GGATG HinfI 49 GAATC G^A[ACGT]TC 405 GATTC MseI 319 TTAA T^TAA 551 TTAA NlaIII 267 CATG CATG^ 333 CATG TfiI 49 GAATC G^A[AT]TC 405 GATTC Tru9I 319 TTAA T^TAA 551 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### BstYI DpnII MboI MboII MflI NdeII Sau3AI SfcI Tsp509I XhoII