(Linear) (Six-base) MAPSORT of: KIEE5267.seq Check: 1197 from: 1 to: 564 KIEE5267.orf With 182 enzymes: SgfI * January 29, 2014 11:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 564 Size: 178 386 AflII C'TTAA_G Cuts at: 0 550 564 Size: 550 14 AlwNI CAG_nnn'CTG Cuts at: 0 298 564 Size: 298 266 ApoI r'AATT_y Cuts at: 0 172 192 564 Size: 172 20 372 Fragments arranged by size: 372 172 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 146 564 Size: 146 418 BclI T'GATC_A Cuts at: 0 234 564 Size: 234 330 BglII A'GATC_T Cuts at: 0 81 322 564 Size: 81 241 242 Fragments arranged by size: 242 241 81 BsaI GGTCTCn'nnnn_ Cuts at: 0 360 564 Size: 360 204 BsaBI GATnn'nnATC Cuts at: 0 233 564 Size: 233 331 BsaWI w'CCGG_w Cuts at: 0 109 564 Size: 109 455 BspEI T'CCGG_A Cuts at: 0 109 564 Size: 109 455 BssSI C'ACGA_G Cuts at: 0 185 564 Size: 185 379 BstYI r'GATC_y Cuts at: 0 81 204 322 564 Size: 81 123 118 242 Fragments arranged by size: 242 123 118 81 BtgI C'CryG_G Cuts at: 0 332 564 Size: 332 232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 564 Size: 178 386 EcoRI G'AATT_C Cuts at: 0 172 564 Size: 172 392 EcoRV GAT'ATC Cuts at: 0 218 564 Size: 218 346 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 357 389 564 Size: 357 32 175 Fragments arranged by size: 357 175 32 HincII GTy'rAC Cuts at: 0 287 564 Size: 287 277 NcoI C'CATG_G Cuts at: 0 332 564 Size: 332 232 SfcI C'TryA_G Cuts at: 0 35 134 290 564 Size: 35 99 156 274 Fragments arranged by size: 274 156 99 35 SmlI C'TyrA_G Cuts at: 0 550 564 Size: 550 14 StyI C'CwwG_G Cuts at: 0 332 564 Size: 332 232 Tth111I GACn'n_nGTC Cuts at: 0 371 564 Size: 371 193 Enzymes that do cut: AcuI AflII AlwNI ApoI BbsI BclI BglII BsaI BsaBI BsaWI BspEI BssSI BstYI BtgI Eco57MI EcoRI EcoRV Hin4I HincII NcoI SfcI SmlI StyI Tth111I Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI EcoNI EcoO109I FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI ZraI