################################## # NO CUT ENZYMES ( 168 enzymes) # ################################## AatII Acc65I AccI AccII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbrPI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MslI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccIII 155 TCCGGA T^CCGGA AcsI 283 AAATTC [GA]^AATT[CT] AcyI 97 GGCGCC G[GA]^CG[CT]C AfaI 135 GTAC GT^AC AluI 435 AGCT AG^CT AlwI 201 GATCC GGATC(4/5) AlwNI 444 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 283 AAATTC [GA]^AATT[CT] BbeI 97 GGCGCC GGCGC^C BbsI 410 GTCTTC GAAGAC(2/6) BbvCI 9 CCTCAGC CCTCAGC(-5/-2) BglII 119 AGATCT A^GATCT BpuAI 410 GTCTTC GAAGAC(2/6) BsaHI 97 GGCGCC G[GA]^CG[CT]C BsaI 72 GGTCTC GGTCTC(1/5) BsaWI 155 TCCGGA [AT]^CCGG[AT] BseAI 155 TCCGGA T^CCGGA BsmFI 111 GTCCC GGGAC(10/14) BspEI 155 TCCGGA T^CCGGA BsrBI 314 CCGCTC CCGCTC(-3/-3) BstAPI 443 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 21 CATCC GGATG(2/0) BstXI 389 CCAACTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 321 GCCAGC GC[ACGT]^[ACGT]GC CfoI 98 GCGC GCG^C DraII 235 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 235 GGGGCCT [GA]G^G[ACGT]CC[CT] FokI 21 CATCC GGATG(9/13) HaeII 97 GGCGCC [GA]GCGC^[CT] HhaI 98 GCGC GCG^C Hin1I 97 GGCGCC G[GA]^CG[CT]C HinP1I 98 GCGC G^CGC HphI 89 TCACC GGTGA(8/7) KasI 97 GGCGCC G^GCGCC MroI 155 TCCGGA T^CCGGA MseI 265 TTAA T^TAA NarI 97 GGCGCC GG^CGCC NlaIII 293 CATG CATG^ RsaI 135 GTAC GT^AC SfaNI 325 GCATC GCATC(5/9) SfoI 97 GGCGCC GGC^GCC TfiI 469 GATTC G^A[AT]TC Tru9I 265 TTAA T^TAA TspRI 406 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BalI 335 TGGCCA TGG^CCA 386 TGGCCA BcnI 69 CCGGG CC^[GC]GG 92 CCGGG BpmI 103 CTGGAG CTGGAG(16/14) 422 CTGGAG BseRI 128 CTCCTC GAGGAG(10/8) 106 GAGGAG BsiYI 301 CCTGGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 415 CCCCAGGCTGG BslI 301 CCTGGCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 415 CCCCAGGCTGG BsmAI 73 GTCTC GTCTC(1/5) 402 GTCTC BstYI 119 AGATCT [GA]^GATC[CT] 200 AGATCC Cfr13I 6 GGCCC G^G[ACGT]CC 236 GGGCC DdeI 10 CTCAG C^T[ACGT]AG 197 CTGAG MflI 119 AGATCT [GA]^GATC[CT] 200 AGATCC MluNI 335 TGGCCA TGG^CCA 386 TGGCCA MscI 335 TGGCCA TGG^CCA 386 TGGCCA NciI 69 CCGGG CC^[GC]GG 92 CCGGG PstI 82 CTGCAG CTGCA^G 441 CTGCAG Sau96I 6 GGCCC G^G[ACGT]CC 236 GGGCC SfcI 82 CTGCAG C^T[GA][CT]AG 441 CTGCAG XhoII 119 AGATCT [GA]^GATC[CT] 200 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### AciI BanI BbvI BsaJI BssKI BstNI DpnII EaeI EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NdeII NlaIV PleI Sau3AI ScrFI TseI Tsp509I