################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BamHI BbeI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspEI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII CpoI DpnII DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HinfI HpaI KasI KpnI KspI MaeIII MboI MfeI MflI MluI MroI MseI MslI MunI NaeI NarI NcoI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tth111I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccI 259 GTCTAC GT^[AC][GT]AC AccII 618 CGCG CG^CG AcyI 625 GACGCC G[GA]^CG[CT]C AfaI 223 GTAC GT^AC AlwNI 509 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 444 CCCGGG C^[CT]CG[GA]G BanI 54 GGTGCC G^G[CT][GA]CC BbrPI 331 CACGTG CAC^GTG BbsI 160 GTCTTC GAAGAC(2/6) BglI 360 GCCTCGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 301 CTGGAG CTGGAG(16/14) BpuAI 160 GTCTTC GAAGAC(2/6) BsaBI 72 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 625 GACGCC G[GA]^CG[CT]C BseRI 663 CTCCTC GAGGAG(10/8) BsmI 65 GCATTC GAATGC(1/-1) BsoBI 444 CCCGGG C^[CT]CG[GA]G BspDI 79 ATCGAT AT^CGAT BspHI 170 TCATGA T^CATGA BsrFI 394 ACCGGC [GA]^CCGG[CT] BssSI 568 CACGAG CACGAG(-5/-1) BstF5I 232 CATCC GGATG(2/0) BstUI 618 CGCG CG^CG Cfr10I 394 ACCGGC [GA]^CCGG[CT] ClaI 79 ATCGAT AT^CGAT DraIII 586 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 399 CCAAGG C^C[AT][AT]GG FauI 561 GCGGG CCCGC(4/6) FokI 232 CATCC GGATG(9/13) Hin1I 625 GACGCC G[GA]^CG[CT]C MamI 72 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspA1I 559 CAGCGG C[AC]G^C[GT]G MvnI 618 CGCG CG^CG NdeI 681 CATATG CA^TATG NspI 210 ACATGC [GA]CATG^[CT] PmaCI 331 CACGTG CAC^GTG PmlI 331 CACGTG CAC^GTG RcaI 170 TCATGA T^CATGA RsaI 223 GTAC GT^AC SfcI 153 CTACAG C^T[GA][CT]AG SmaI 444 CCCGGG CCC^GGG SpeI 219 ACTAGT A^CTAGT StyI 399 CCAAGG C^C[AT][AT]GG TaqI 80 TCGA T^CGA Tsp509I 118 AATT ^AATT TthHB8I 80 TCGA T^CGA XcmI 29 CCAGCCCCTACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 444 CCCGGG C^CCGGG XmaI 444 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AciI 639 CCGC CCGC(-3/-1) 561 GCGG AluI 527 AGCT AG^CT 596 AGCT ApaI 441 GGGCCC GGGCC^C 554 GGGCCC BalI 5 TGGCCA TGG^CCA 335 TGGCCA BfaI 197 CTAG C^TAG 220 CTAG BsaAI 37 TACGTG [CT]AC^GT[GA] 331 CACGTG BsmFI 279 GTCCC GGGAC(10/14) 413 GTCCC BsrI 105 ACTGG ACTGG(1/-1) 59 CCAGT BstNI 508 CCAGG CC^[AT]GG 514 CCTGG Cac8I 23 GCAGGC GC[ACGT]^[ACGT]GC 296 GCCTGC CfoI 15 GCGC GCG^C 421 GCGC DdeI 88 CTCAG C^T[ACGT]AG 540 CTCAG EarI 634 CTCTTC CTCTTC(1/4) 652 CTCTTC EcoRII 508 CCAGG ^CC[AT]GG 514 CCTGG HgaI 625 GACGC GACGC(5/10) 158 GCGTC HhaI 15 GCGC GCG^C 421 GCGC HinP1I 15 GCGC G^CGC 421 GCGC Ksp632I 634 CTCTTC CTCTTC(1/4) 652 CTCTTC MaeI 197 CTAG C^TAG 220 CTAG MluNI 5 TGGCCA TGG^CCA 335 TGGCCA MscI 5 TGGCCA TGG^CCA 335 TGGCCA MvaI 508 CCAGG CC^[AT]GG 514 CCTGG PflMI 508 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 615 CCACGCGATGG SapI 633 GCTCTTC GCTCTTC(1/4) 651 GCTCTTC Van91I 508 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 615 CCACGCGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### BanII BbvI BcnI BmyI BsaI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspMI BssKI Cfr13I DraII EaeI EcoO109I Fnu4HI HaeIII HapII HpaII HphI ItaI MaeII MboII MnlI MspI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TseI TspRI