Restriction map for
pFN21AE5288
(Circular) (Six-base) MAPSORT of: pFN21AE5288.seq Check: 7450 from: 1 to: 5404
pFN21AE5288 5404 bp
With 182 enzymes: SgfI *
January 30, 2014 17:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2013 2337 2478 2013
Size: 324 141 4939
Fragments arranged by size:
4939 324 141
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2265 2747 1937
Size: 328 482 4594
Fragments arranged by size:
4594 482 328
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3467 4851 5119 1632
Size: 1835 1384 268 1917
Fragments arranged by size:
1917 1835 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 4303 5280 1203
Size: 3100 977 1327
Fragments arranged by size:
3100 1327 977
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5404
AlwNI CAG_nnn'CTG
Cuts at: 2519 3147 4719 2519
Size: 628 1572 3204
Fragments arranged by size:
3204 1572 628
ApaI G_GGCC'C
Cuts at: 2450 2563 2450
Size: 113 5291
ApaLI G'TGCA_C
Cuts at: 3461 4617 3461
Size: 1156 4248
ApoI r'AATT_y
Cuts at: 1493 2713 2939 3091 5200 1493
Size: 1220 226 152 2109 1697
Fragments arranged by size:
2109 1697 1220 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2449 2729 1870
Size: 73 506 280 4545
Fragments arranged by size:
4545 506 280 73
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2059 2725 4180 618
Size: 325 217 627 272 666 1455 1842
Fragments arranged by size:
1842 1455 666 627 325 272 217
BanII G_rGCy'C
Cuts at: 1391 2450 2546 2563 2723 1391
Size: 1059 96 17 160 4072
Fragments arranged by size:
4072 1059 160 96 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2158 928
Size: 971 259 4174
Fragments arranged by size:
4174 971 259
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3595 3629 1903
Size: 34 1658 34 3678
Fragments arranged by size:
3678 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2371 4015
Size: 107 122 71 631 605 337 362 1644
Cuts at: 4015 136
Size: 1525
Fragments arranged by size:
1644 1525 631 605 362 337 122 107
71
BglII A'GATC_T
Cuts at: 5399 5399
Size: 5404
BlpI GC'TnA_GC
Cuts at: 2821 2821
Size: 5404
Bme1580I G_kGCm'C
Cuts at: 1790 2064 2131 2450 2563 3465 4621 1790
Size: 274 67 319 113 902 1156 2573
Fragments arranged by size:
2573 1156 902 319 274 113 67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2058 3135 4093
Size: 416 53 83 399 24 641 1077 958
Cuts at: 4093 442
Size: 1753
Fragments arranged by size:
1753 1077 958 641 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2327 4064 1239
Size: 1088 1737 2579
Fragments arranged by size:
2579 1737 1088
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3529 4394 4692 3529
Size: 865 298 4241
Fragments arranged by size:
4241 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2241 2303 2459 4067 882
Size: 617 742 62 156 1608 2219
Fragments arranged by size:
2219 1608 742 617 156 62
BsaAI yAC'GTr
Cuts at: 493 1204 2044 2338 3195 493
Size: 711 840 294 857 2702
Fragments arranged by size:
2702 857 840 711 294
BsaBI GATnn'nnATC
Cuts at: 2081 5398 2081
Size: 3317 2087
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2631 3211 3593 4228
Size: 53 83 186 936 1098 580 382 635
Cuts at: 4228 275
Size: 1451
Fragments arranged by size:
1451 1098 936 635 580 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3837 4235 4509 4656 5067 3837
Size: 398 274 147 411 4174
Fragments arranged by size:
4174 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2401 2431 1178
Size: 30 165 30 998 30 4151
Fragments arranged by size:
4151 998 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2659 1186
Size: 1473 3931
BseYI C'CCAG_C
Cuts at: 2033 2152 2194 2349 2562 4607 5103 2033
Size: 119 42 155 213 2045 496 2334
Fragments arranged by size:
2334 2045 496 213 155 119 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2047 2318 2375 2380 2630 1163
Size: 573 311 271 57 5 250 3937
Fragments arranged by size:
3937 573 311 271 250 57 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3615 4643 1540
Size: 275 186 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3465 3550 4621 3465
Size: 85 1071 4248
Fragments arranged by size:
4248 1071 85
BsmI GAATG_Cn'
Cuts at: 2070 2915 3008 2070
Size: 845 93 4466
Fragments arranged by size:
4466 845 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2131 2450 2546 2563 2723 3465
Size: 399 274 67 319 96 17 160 742
Cuts at: 3465 3550 4621 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 319 274 160 96
85 67 17
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 2175 3293 5023 2175
Size: 1118 1730 2556
Fragments arranged by size:
2556 1730 1118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2013 2112 2337 2478 2661 2759 844
Size: 1169 99 225 141 183 98 3489
Fragments arranged by size:
3489 1169 225 183 141 99 98
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 1394
Size: 429 1468 3507
Fragments arranged by size:
3507 1468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3899 4073 65
Size: 1183 2651 174 1396
Fragments arranged by size:
2651 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2399 4048 4235 1700
Size: 110 589 1649 187 2869
Fragments arranged by size:
2869 1649 589 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5404
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 2573 3464 4476 2573
Size: 891 1012 3501
Fragments arranged by size:
3501 1012 891
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5404
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2734 3101 3486 3503 4955 5041 5053 5399
Size: 762 367 385 17 1452 86 12 346
Cuts at: 5399 1972
Size: 1977
Fragments arranged by size:
1977 1452 762 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2639 487
Size: 683 56 1413 3252
Fragments arranged by size:
3252 1413 683 56
BtsI GCAGTG_nn'
Cuts at: 2392 2421 2920 3714 3734 2392
Size: 29 499 794 20 4062
Fragments arranged by size:
4062 794 499 29 20
ClaI AT'CG_AT
Cuts at: 1554 2085 1554
Size: 531 4873
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DraIII CAC_nnn'GTG
Cuts at: 2596 2596
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 2340 2401
Size: 54 1425 50 275 18 180 330 61
Cuts at: 2401 2851 3740 5369 8
Size: 450 889 1629 43
Fragments arranged by size:
1629 1425 889 450 330 275 180 61
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2851 1537
Size: 275 1039 4090
Fragments arranged by size:
4090 1039 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 2663 3334 1353
Size: 1292 18 671 3423
Fragments arranged by size:
3423 1292 671 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2633 3991 4365 4511 387
Size: 1064 138 1044 1358 374 146 1280
Fragments arranged by size:
1358 1280 1064 1044 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2327 3467 4064 4851 5119 1239
Size: 393 695 1140 597 787 268 1524
Fragments arranged by size:
1524 1140 787 695 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2446 2516 2559 2446
Size: 70 43 5291
Fragments arranged by size:
5291 70 43
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 4551 1678
Size: 2873 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2401 2433 4045 4077 4119
Size: 32 135 32 829 32 1612 32 42
Cuts at: 4119 4151 1373
Size: 32 2626
Fragments arranged by size:
2626 1612 829 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 2994 669
Size: 988 281 810 246 3079
Fragments arranged by size:
3079 988 810 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 3003 3003
Size: 5404
MluI A'CGCG_T
Cuts at: 5280 5280
Size: 5404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2012 2342 10
Size: 54 1425 343 180 330 3072
Fragments arranged by size:
3072 1425 343 330 180 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 3721 3880 518
Size: 648 2196 359 159 2042
Fragments arranged by size:
2196 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2566 2820 3497 4645 4890 1606
Size: 960 254 677 1148 245 2120
Fragments arranged by size:
2120 1148 960 677 254 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
NdeI CA'TA_TG
Cuts at: 387 2687 387
Size: 2300 3104
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2219 2762 4307 1132
Size: 97 990 543 1545 2229
Fragments arranged by size:
2229 1545 990 543 97
PasI CC'CwG_GG
Cuts at: 1348 2519 1348
Size: 1171 4233
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2519 2626 1108
Size: 1411 107 3886
Fragments arranged by size:
3886 1411 107
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PmlI CAC'GTG
Cuts at: 2338 2338
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5404
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PspOMI G'GGCC_C
Cuts at: 2446 2559 2446
Size: 113 5291
PstI C_TGCA'G
Cuts at: 830 2756 830
Size: 1926 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SapI GCTCTTCn'nnn_
Cuts at: 2645 2663 2645
Size: 18 5386
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2158 2752 3887 4568 4759 826
Size: 219 1113 594 1135 681 191 1471
Fragments arranged by size:
1471 1135 1113 681 594 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2451 2731 1872
Size: 579 280 4545
Fragments arranged by size:
4545 579 280
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3508 4409 4671 820
Size: 197 926 1565 901 262 1553
Fragments arranged by size:
1565 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 2224 152
Size: 2072 3332
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5404
StyI C'CwwG_G
Cuts at: 513 1065 2004 2404 2843 513
Size: 552 939 400 439 3074
Fragments arranged by size:
3074 939 552 439 400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2339 2363 2480 2596 3431 3448 3601
Size: 1255 759 24 117 116 835 17 153
Cuts at: 3601 3786 4231 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 835 759 445 185 153 117
116 24 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3650 5362 96
Size: 274 80 33 51 1443 1673 1712 138
Fragments arranged by size:
1712 1673 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5364 5396 5364
Size: 32 5372
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2041 2041
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5404
XmaI C'CCGG_G
Cuts at: 1870 2449 2729 1870
Size: 579 280 4545
Fragments arranged by size:
4545 579 280
XmnI GAAnn'nnTTC
Cuts at: 3533 3533
Size: 5404
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI
SrfI SwaI