Restriction map for
pFN21AE5288
(Linear) (Six-base) MAPSORT of: KIEE5288.seq Check: 1125 from: 1 to: 699
KIEE5288.orf
With 182 enzymes: SgfI *
January 29, 2014 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 8 332 473 699
Size: 8 324 141 226
Fragments arranged by size:
324 226 141 8
AccI GT'mk_AC
Cuts at: 0 260 699
Size: 260 439
AlwNI CAG_nnn'CTG
Cuts at: 0 514 699
Size: 514 185
ApaI G_GGCC'C
Cuts at: 0 445 558 699
Size: 445 113 141
Fragments arranged by size:
445 141 113
AvaI C'yCGr_G
Cuts at: 0 444 699
Size: 444 255
BanI G'GyrC_C
Cuts at: 0 54 699
Size: 54 645
BanII G_rGCy'C
Cuts at: 0 445 541 558 699
Size: 445 96 17 141
Fragments arranged by size:
445 141 96 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 153 699
Size: 153 546
BglI GCCn_nnn'nGGC
Cuts at: 0 366 699
Size: 366 333
Bme1580I G_kGCm'C
Cuts at: 0 59 126 445 558 699
Size: 59 67 319 113 141
Fragments arranged by size:
319 141 113 67 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 53 699
Size: 53 646
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 699
Size: 322 377
BsaI GGTCTCn'nnnn_
Cuts at: 0 236 298 454 699
Size: 236 62 156 245
Fragments arranged by size:
245 236 156 62
BsaAI yAC'GTr
Cuts at: 0 39 333 699
Size: 39 294 366
Fragments arranged by size:
366 294 39
BsaBI GATnn'nnATC
Cuts at: 0 76 699
Size: 76 623
BsaHI Gr'CG_yC
Cuts at: 0 626 699
Size: 626 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 396 426 699
Size: 396 30 273
Fragments arranged by size:
396 273 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 654 699
Size: 654 45
BseYI C'CCAG_C
Cuts at: 0 28 147 189 344 557 699
Size: 28 119 42 155 213 142
Fragments arranged by size:
213 155 142 119 42 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 313 370 375 625 699
Size: 42 271 57 5 250 74
Fragments arranged by size:
271 250 74 57 42 5
BsmI GAATG_Cn'
Cuts at: 0 65 699
Size: 65 634
Bsp1286I G_dGCh'C
Cuts at: 0 59 126 445 541 558 699
Size: 59 67 319 96 17 141
Fragments arranged by size:
319 141 96 67 59 17
BspHI T'CATG_A
Cuts at: 0 170 699
Size: 170 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 8 107 332 473 656 699
Size: 8 99 225 141 183 43
Fragments arranged by size:
225 183 141 99 43 8
BsrFI r'CCGG_y
Cuts at: 0 394 699
Size: 394 305
BssSI C'ACGA_G
Cuts at: 0 568 699
Size: 568 131
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 634 699
Size: 634 65
BtsI GCAGTG_nn'
Cuts at: 0 387 416 699
Size: 387 29 283
Fragments arranged by size:
387 283 29
ClaI AT'CG_AT
Cuts at: 0 80 699
Size: 80 619
DraIII CAC_nnn'GTG
Cuts at: 0 591 699
Size: 591 108
EaeI y'GGCC_r
Cuts at: 0 5 335 396 699
Size: 5 330 61 303
Fragments arranged by size:
330 303 61 5
EarI CTCTTCn'nnn_
Cuts at: 0 640 658 699
Size: 640 18 41
Fragments arranged by size:
640 41 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 628 699
Size: 628 71
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 699
Size: 322 377
EcoO109I rG'GnC_Cy
Cuts at: 0 441 511 554 699
Size: 441 70 43 145
Fragments arranged by size:
441 145 70 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 396 428 699
Size: 396 32 271
Fragments arranged by size:
396 271 32
MscI TGG'CCA
Cuts at: 0 7 337 699
Size: 7 330 362
Fragments arranged by size:
362 330 7
MspA1I CmG'CkG
Cuts at: 0 561 699
Size: 561 138
NdeI CA'TA_TG
Cuts at: 0 682 699
Size: 682 17
NspI r_CATG'y
Cuts at: 0 214 699
Size: 214 485
PasI CC'CwG_GG
Cuts at: 0 514 699
Size: 514 185
PflMI CCAn_nnn'nTGG
Cuts at: 0 514 621 699
Size: 514 107 78
Fragments arranged by size:
514 107 78
PmlI CAC'GTG
Cuts at: 0 333 699
Size: 333 366
PspOMI G'GGCC_C
Cuts at: 0 441 554 699
Size: 441 113 145
Fragments arranged by size:
441 145 113
SapI GCTCTTCn'nnn_
Cuts at: 0 640 658 699
Size: 640 18 41
Fragments arranged by size:
640 41 18
SfcI C'TryA_G
Cuts at: 0 153 699
Size: 153 546
SmaI CCC'GGG
Cuts at: 0 446 699
Size: 446 253
SpeI A'CTAG_T
Cuts at: 0 219 699
Size: 219 480
StyI C'CwwG_G
Cuts at: 0 399 699
Size: 399 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 358 475 591 699
Size: 334 24 117 116 108
Fragments arranged by size:
334 117 116 108 24
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 36 699
Size: 36 663
XmaI C'CCGG_G
Cuts at: 0 444 699
Size: 444 255
Enzymes that do cut:
AarI AccI AlwNI ApaI AvaI BanI BanII BbsI
BglI Bme1580I BmrI BpmI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI
BsrFI BssSI BtgZI BtsI ClaI DraIII EaeI EarI
EciI Eco57MI EcoO109I Hin4I MscI MspA1I NdeI NspI
PasI PflMI PmlI PspOMI SapI SfcI SmaI SpeI
StyI TaqII XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I
BpuEI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI CspCI DraI DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MslI NaeI NarI NcoI NgoMIV NheI NotI NruI
NsiI PacI PciI PfoI PmeI PpiI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmlI SnaBI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI