################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanII BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI BstYI Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HgaI HindIII HpaI HphI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI NaeI NciI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tsp45I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 411 GTATAC GT^[AC][GT]AC AccII 75 CGCG CG^CG AcyI 34 GGCGCC G[GA]^CG[CT]C AfaI 423 GTAC GT^AC AlwI 400 GGATC GGATC(4/5) BanI 34 GGCGCC G^G[CT][GA]CC BbeI 34 GGCGCC GGCGC^C BbsI 469 GAAGAC GAAGAC(2/6) BfaI 707 CTAG C^TAG BpmI 332 CTGGAG CTGGAG(16/14) BpuAI 469 GAAGAC GAAGAC(2/6) BsaHI 34 GGCGCC G[GA]^CG[CT]C BsiYI 427 CCAGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 427 CCAGAAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 755 GTCTC GTCTC(1/5) BsmFI 116 GGGAC GGGAC(10/14) BsmI 273 GAATGC GAATGC(1/-1) BsrBI 21 GAGCGG CCGCTC(-3/-3) Bst1107I 411 GTATAC GTA^TAC BstUI 75 CGCG CG^CG BstZ17I 411 GTATAC GTA^TAC EaeI 5 CGGCCG [CT]^GGCC[GA] EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG HaeII 34 GGCGCC [GA]GCGC^[CT] HapII 38 CCGG C^CGG Hin1I 34 GGCGCC G[GA]^CG[CT]C HincII 462 GTTGAC GT[CT]^[GA]AC HindII 462 GTTGAC GT[CT]^[GA]AC HpaII 38 CCGG C^CGG KasI 34 GGCGCC G^GCGCC MaeI 707 CTAG C^TAG MaeII 654 ACGT A^CGT MspI 38 CCGG C^CGG MvnI 75 CGCG CG^CG MwoI 10 GCCCTGGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NarI 34 GGCGCC GG^CGCC NlaIII 288 CATG CATG^ NlaIV 34 GGCGCC GG[ACGT]^[ACGT]CC NotI 4 GCGGCCGC GC^GGCCGC RsaI 423 GTAC GT^AC SfoI 34 GGCGCC GGC^GCC SmlI 416 CTCAAG C^T[CT][GA]AG TaqI 536 TCGA T^CGA TthHB8I 536 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 240 AAATTT [GA]^AATT[CT] 559 GAATTT ApoI 240 AAATTT [GA]^AATT[CT] 559 GAATTT BbvI 507 GCAGC GCAGC(8/12) 100 GCTGC BsaJI 11 CCCTGG C^C[ACGT][ACGT]GG 12 CCTGGG BsiEI 5 CGGCCG CG[GA][CT]^CG 82 CGGTCG BssKI 12 CCTGG ^CC[ACGT]GG 70 CCTGG BstF5I 761 CATCC GGATG(2/0) 372 GGATG BstNI 12 CCTGG CC^[AT]GG 70 CCTGG Cac8I 19 GCGAGC GC[ACGT]^[ACGT]GC 31 GCAGGC CfoI 35 GCGC GCG^C 74 GCGC EcoRII 12 CCTGG ^CC[AT]GG 70 CCTGG FokI 761 CATCC GGATG(9/13) 372 GGATG HaeIII 6 GGCC GG^CC 448 GGCC HhaI 35 GCGC GCG^C 74 GCGC HinP1I 35 GCGC G^CGC 74 GCGC MvaI 12 CCTGG CC^[AT]GG 70 CCTGG ScrFI 12 CCTGG CC^[ACGT]GG 70 CCTGG TseI 100 GCTGC G^C[AT]GC 507 GCAGC TspRI 120 CAGTG CA[GC]TG[ACGT][ACGT]^ 516 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 20 enzymes) # ######################################### AciI AluI BseRI BsrI DdeI DpnII EarI Fnu4HI HinfI ItaI Ksp632I MaeIII MboI MboII MnlI NdeII SapI Sau3AI TfiI Tsp509I