(Linear) (Six-base) MAPSORT of: KIEE5335.seq  Check: 7336  from: 1  to: 804



KIEE5335.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:56  ..

AccI GT'mk_AC

Cuts at:      0     412     804 
   Size:        412     392
 
ApoI r'AATT_y

Cuts at:      0     240     559     804 
   Size:        240     319     245

  Fragments arranged by size: 

                 319     245     240
 
BanI G'GyrC_C

Cuts at:      0      34     804 
   Size:         34     770
 
BbeI G_GCGC'C

Cuts at:      0      38     804 
   Size:         38     766
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     476     804 
   Size:        476     328
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     123     804 
   Size:        123     681
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     804 
   Size:        353     451
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     401     804 
   Size:        401     403
 
BsaHI Gr'CG_yC

Cuts at:      0      35     804 
   Size:         35     769
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      41     514     517     804 
   Size:         41     473       3     287

  Fragments arranged by size: 

                 473     287      41       3
 
BsiEI CG_ry'CG

Cuts at:      0       8      85     804 
   Size:          8      77     719

  Fragments arranged by size: 

                 719      77       8
 
BsmI GAATG_Cn'

Cuts at:      0     279     804 
   Size:        279     525
 
BsrBI CCG'CTC

Cuts at:      0      23     804 
   Size:         23     781
 
BstZ17I GTA'TAC

Cuts at:      0     413     804 
   Size:        413     391
 
EaeI y'GGCC_r

Cuts at:      0       5     804 
   Size:          5     799
 
EagI C'GGCC_G

Cuts at:      0       5     804 
   Size:          5     799
 
EarI CTCTTCn'nnn_

Cuts at:      0      90     188     485     804 
   Size:         90      98     297     319

  Fragments arranged by size: 

                 319     297      98      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     804 
   Size:        353     451
 
HaeII r_GCGC'y

Cuts at:      0      38     804 
   Size:         38     766
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174     625     657     804 
   Size:        142      32     451      32     147

  Fragments arranged by size: 

                 451     147     142      32      32
 
HincII GTy'rAC

Cuts at:      0     464     804 
   Size:        464     340
 
KasI G'GCGC_C

Cuts at:      0      34     804 
   Size:         34     770
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     158     804 
   Size:        158     646
 
NarI GG'CG_CC

Cuts at:      0      35     804 
   Size:         35     769
 
NotI GC'GGCC_GC

Cuts at:      0       5     804 
   Size:          5     799
 
PasI CC'CwG_GG

Cuts at:      0      12     804 
   Size:         12     792
 
SapI GCTCTTCn'nnn_

Cuts at:      0      90     188     485     804 
   Size:         90      98     297     319

  Fragments arranged by size: 

                 319     297      98      90
 
SfoI GGC'GCC

Cuts at:      0      36     804 
   Size:         36     768
 
SmlI C'TyrA_G

Cuts at:      0     416     804 
   Size:        416     388
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      70     102     804 
   Size:         70      32     702

  Fragments arranged by size: 

                 702      70      32
 

 Enzymes that do cut:

     AccI     ApoI     BanI     BbeI     BbsI     BmrI     BpmI    BpuEI
    BsaHI    BseRI    BsiEI     BsmI    BsrBI  BstZ17I     EaeI     EagI
     EarI  Eco57MI    HaeII    Hin4I   HincII     KasI     MmeI     NarI
     NotI     PasI     SapI     SfoI     SmlI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI     NspI
     PacI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfcI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI