(Circular) (Six-base) MAPSORT of: pFN21AE6646.seq Check: 8529 from: 1 to: 5383 pFN21AE6646 5383 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 1999 2354 1999 Size: 355 5028 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3193 278 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2726 1937 Size: 789 4594 Acc65I G'GTAC_C Cuts at: 2619 2704 2619 Size: 85 5298 AclI AA'CG_TT Cuts at: 1563 3510 3883 1563 Size: 1947 373 3063 Fragments arranged by size: 3063 1947 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2684 3446 4830 5098 1632 Size: 1052 762 1384 268 1917 Fragments arranged by size: 1917 1384 1052 762 268 AfeI AGC'GCT Cuts at: 2596 2596 Size: 5383 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5186 AflIII A'CryG_T Cuts at: 1203 4282 5259 1203 Size: 3079 977 1327 Fragments arranged by size: 3079 1327 977 AgeI A'CCGG_T Cuts at: 4214 4214 Size: 5383 AhdI GACnn_n'nnGTC Cuts at: 4112 4112 Size: 5383 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5383 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5383 AlwNI CAG_nnn'CTG Cuts at: 3126 4698 3126 Size: 1572 3811 ApaLI G'TGCA_C Cuts at: 3440 4596 3440 Size: 1156 4227 ApoI r'AATT_y Cuts at: 1493 2443 2692 2918 3070 5179 1493 Size: 950 249 226 152 2109 1697 Fragments arranged by size: 2109 1697 950 249 226 152 AseI AT'TA_AT Cuts at: 160 1032 3937 160 Size: 872 2905 1606 Fragments arranged by size: 2905 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 AvaI C'yCGr_G Cuts at: 1870 1943 2708 1870 Size: 73 765 4545 Fragments arranged by size: 4545 765 73 BamHI G'GATC_C Cuts at: 2713 3080 2713 Size: 367 5016 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2318 2619 2704 4159 618 Size: 325 217 627 531 301 85 1455 1842 Fragments arranged by size: 1842 1455 627 531 325 301 217 85 BanII G_rGCy'C Cuts at: 1391 2702 1391 Size: 1311 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4412 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3574 3608 1903 Size: 34 50 34 1553 34 3678 Fragments arranged by size: 3678 1553 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 4485 3275 Size: 1210 4173 BclI T'GATC_A Cuts at: 1548 1548 Size: 5383 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3994 136 Size: 107 122 71 631 605 2322 1525 Fragments arranged by size: 2322 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5378 5378 Size: 5383 BlpI GC'TnA_GC Cuts at: 2800 2800 Size: 5383 Bme1580I G_kGCm'C Cuts at: 1790 3444 4600 1790 Size: 1654 1156 2573 Fragments arranged by size: 2573 1654 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5383 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2118 2569 3114 Size: 416 53 83 399 24 701 451 545 Cuts at: 3114 4072 442 Size: 958 1753 Fragments arranged by size: 1753 958 701 545 451 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5383 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2568 2568 Size: 5383 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2361 4043 1239 Size: 1122 1682 2579 Fragments arranged by size: 2579 1682 1122 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3508 4373 4671 3508 Size: 865 298 4220 Fragments arranged by size: 4220 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4046 882 Size: 617 2547 2219 Fragments arranged by size: 2547 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3174 493 Size: 711 1970 2702 Fragments arranged by size: 2702 1970 711 BsaBI GATnn'nnATC Cuts at: 5377 5377 Size: 5383 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3190 3572 4207 275 Size: 53 83 186 936 1657 382 635 1451 Fragments arranged by size: 1657 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3816 4214 4488 4635 5046 3816 Size: 398 274 147 411 4153 Fragments arranged by size: 4153 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5158 Fragments arranged by size: 5158 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5383 BseYI C'CCAG_C Cuts at: 2213 4586 5082 2213 Size: 2373 496 2514 Fragments arranged by size: 2514 2373 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2382 1163 Size: 573 646 4164 Fragments arranged by size: 4164 646 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2833 3594 4622 1540 Size: 275 186 832 761 1028 2301 Fragments arranged by size: 2301 1028 832 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 3444 3529 4600 2493 Size: 951 85 1071 3276 Fragments arranged by size: 3276 1071 951 85 BsmI GAATG_Cn' Cuts at: 2231 2674 2894 2987 2231 Size: 443 220 93 4627 Fragments arranged by size: 4627 443 220 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2493 2702 3444 3529 4600 1391 Size: 399 703 209 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 703 399 209 85 BspEI T'CCGG_A Cuts at: 5046 5046 Size: 5383 BspHI T'CATG_A Cuts at: 3272 5002 3272 Size: 1730 3653 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 1999 2354 2738 844 Size: 1155 355 384 3489 Fragments arranged by size: 3489 1155 384 355 BsrBI CCG'CTC Cuts at: 1394 1823 3270 1394 Size: 429 1447 3507 Fragments arranged by size: 3507 1447 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3878 4052 65 Size: 1183 2630 174 1396 Fragments arranged by size: 2630 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4027 4214 1700 Size: 110 2217 187 2869 Fragments arranged by size: 2869 2217 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5383 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5383 BssSI C'ACGA_G Cuts at: 3443 4455 3443 Size: 1012 4371 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5383 BstBI TT'CG_AA Cuts at: 4187 4187 Size: 5383 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5383 BstYI r'GATC_y Cuts at: 1972 2456 2713 3080 3465 3482 4934 5020 5032 Size: 484 257 367 385 17 1452 86 12 Cuts at: 5032 5378 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 484 385 367 346 257 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4831 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4644 Fragments arranged by size: 4644 683 56 BtsI GCAGTG_nn' Cuts at: 2236 2344 2899 3693 3713 2236 Size: 108 555 794 20 3906 Fragments arranged by size: 3906 794 555 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5383 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5348 DraI TTT'AAA Cuts at: 2687 2687 Size: 5383 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4390 809 Size: 507 3074 1802 Fragments arranged by size: 3074 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2419 2830 Size: 54 1425 50 275 18 273 316 411 Cuts at: 2830 3719 5348 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 411 316 275 273 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2830 1537 Size: 275 1018 4090 Fragments arranged by size: 4090 1018 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2417 3313 1353 Size: 1064 896 3423 Fragments arranged by size: 3423 1064 896 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3970 4344 4490 387 Size: 1064 138 2381 374 146 1280 Fragments arranged by size: 2381 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2361 2684 3446 4043 4830 5098 1239 Size: 393 729 323 762 597 787 268 1524 Fragments arranged by size: 1524 787 762 729 597 393 323 268 EcoRI G'AATT_C Cuts at: 2692 3070 2692 Size: 378 5005 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2428 2428 Size: 5383 FspI TGC'GCA Cuts at: 3889 3889 Size: 5383 HaeII r_GCGC'y Cuts at: 1678 2598 4530 1678 Size: 920 1932 2531 Fragments arranged by size: 2531 1932 920 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2448 2480 4024 4056 4098 Size: 32 135 32 876 32 1544 32 42 Cuts at: 4098 4130 1373 Size: 32 2626 Fragments arranged by size: 2626 1544 876 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2284 2631 2727 2973 669 Size: 988 281 346 347 96 246 3079 Fragments arranged by size: 3079 988 347 346 281 246 96 HindIII A'AGCT_T Cuts at: 748 748 Size: 5383 HpaI GTT'AAC Cuts at: 2973 2973 Size: 5383 KpnI G_GTAC'C Cuts at: 2623 2708 2623 Size: 85 5298 MfeI C'AATT_G Cuts at: 2982 2982 Size: 5383 MluI A'CGCG_T Cuts at: 5259 5259 Size: 5383 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4497 4681 1305 Size: 3192 184 2007 Fragments arranged by size: 3192 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2105 2421 10 Size: 54 1425 343 273 316 2972 Fragments arranged by size: 2972 1425 343 316 273 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3341 3700 3859 518 Size: 648 843 1332 359 159 2042 Fragments arranged by size: 2042 1332 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2799 3476 4624 4869 1606 Size: 1193 677 1148 245 2120 Fragments arranged by size: 2120 1193 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4831 NdeI CA'TA_TG Cuts at: 387 387 Size: 5383 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5383 NotI GC'GGCC_GC Cuts at: 2830 2830 Size: 5383 NspI r_CATG'y Cuts at: 1132 1229 2741 4286 1132 Size: 97 1512 1545 2229 Fragments arranged by size: 2229 1545 1512 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5383 PciI A'CATG_T Cuts at: 4282 4282 Size: 5383 PflMI CCAn_nnn'nTGG Cuts at: 1108 2133 1108 Size: 1025 4358 PmeI GTTT'AAAC Cuts at: 2687 2687 Size: 5383 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3452 3484 4965 4997 3452 Size: 32 1481 32 3838 Fragments arranged by size: 3838 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5383 PsiI TTA'TAA Cuts at: 2953 2953 Size: 5383 PstI C_TGCA'G Cuts at: 830 2735 830 Size: 1905 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5383 SalI G'TCGA_C Cuts at: 1936 2725 1936 Size: 789 4594 SapI GCTCTTCn'nnn_ Cuts at: 2417 2417 Size: 5383 SbfI CC_TGCA'GG Cuts at: 2735 2735 Size: 5383 ScaI AGT'ACT Cuts at: 3631 5343 3631 Size: 1712 3671 SfcI C'TryA_G Cuts at: 826 1045 2731 3866 4547 4738 826 Size: 219 1686 1135 681 191 1471 Fragments arranged by size: 1686 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5383 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5383 SmaI CCC'GGG Cuts at: 1872 2710 1872 Size: 838 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3487 4388 4650 820 Size: 197 926 1544 901 262 1553 Fragments arranged by size: 1553 1544 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3174 493 Size: 2681 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5383 SphI G_CATG'C Cuts at: 1132 2741 1132 Size: 1609 3774 SspI AAT'ATT Cuts at: 5 52 2180 3307 5 Size: 47 2128 1127 2081 Fragments arranged by size: 2128 2081 1127 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5383 StyI C'CwwG_G Cuts at: 513 1065 2822 513 Size: 552 1757 3074 Fragments arranged by size: 3074 1757 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3410 3427 3580 3765 4210 325 Size: 1255 1830 17 153 185 445 1498 Fragments arranged by size: 1830 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2567 3629 5341 Size: 274 80 33 51 1443 590 1062 1712 Cuts at: 5341 96 Size: 138 Fragments arranged by size: 1712 1443 1062 590 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5343 5375 5343 Size: 32 5351 Tth111I GACn'n_nGTC Cuts at: 1316 2635 1316 Size: 1319 4064 XbaI T'CTAG_A Cuts at: 2719 2719 Size: 5383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2385 2385 Size: 5383 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5383 XmaI C'CCGG_G Cuts at: 1870 2708 1870 Size: 838 4545 XmnI GAAnn'nnTTC Cuts at: 2411 3512 2411 Size: 1101 4282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3191 276 Size: 53 83 186 936 1657 2468 Fragments arranged by size: 2468 1657 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI