Restriction map for
pFN21AE6276
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsiYI BslI BsmAI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI BstXI Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII HphI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI SwaI Tth111I Van91I XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AlwI 275 GGATC GGATC(4/5)
AlwNI 418 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 200 GAGCAC G[AT]GC[AT]^C
AvaII 100 GGACC G^G[AT]CC
BbvI 328 GCAGC GCAGC(8/12)
BmyI 200 GAGCAC G[AGT]GC[ACT]^C
BsaAI 345 CACGTA [CT]AC^GT[GA]
BsiHKAI 200 GAGCAC G[AT]GC[AT]^C
BsmFI 426 GTCCC GGGAC(10/14)
Bsp1286I 200 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 459 TGTACA T^GTACA
BsrGI 459 TGTACA T^GTACA
BssKI 27 CCTGG ^CC[ACGT]GG
Bst1107I 412 GTATAC GTA^TAC
BstF5I 653 CATCC GGATG(2/0)
BstNI 27 CCTGG CC^[AT]GG
BstYI 275 GGATCT [GA]^GATC[CT]
BstZ17I 412 GTATAC GTA^TAC
Cac8I 157 GCCTGC GC[ACGT]^[ACGT]GC
DpnII 276 GATC ^GATC
DraI 87 TTTAAA TTT^AAA
DraII 99 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 284 CTCTTC CTCTTC(1/4)
EcoO109I 99 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 27 CCTGG ^CC[AT]GG
EcoT14I 644 CCTTGG C^C[AT][AT]GG
Fnu4HI 328 GCAGC GC^[ACGT]GC
FokI 653 CATCC GGATG(9/13)
ItaI 328 GCAGC GC^[ACGT]GC
Ksp632I 284 CTCTTC CTCTTC(1/4)
MaeIII 343 GTCAC ^GT[ACGT]AC
MboI 276 GATC ^GATC
MflI 275 GGATCT [GA]^GATC[CT]
MspA1I 503 CAGCTG C[AC]G^C[GT]G
MvaI 27 CCTGG CC^[AT]GG
NdeII 276 GATC ^GATC
NlaIV 648 GGAGCC GG[ACGT]^[ACGT]CC
NspI 544 GCATGT [GA]CATG^[CT]
PleI 524 GACTC GAGTC(4/5)
PpuMI 99 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 503 CAGCTG CAG^CTG
Sau3AI 276 GATC ^GATC
ScaI 213 AGTACT AGT^ACT
ScrFI 27 CCTGG CC^[ACGT]GG
SfaNI 325 GATGC GCATC(5/9)
SfcI 119 CTACAG C^T[GA][CT]AG
SspBI 459 TGTACA T^GTACA
SspI 622 AATATT AAT^ATT
StyI 644 CCTTGG C^C[AT][AT]GG
TaqI 210 TCGA T^CGA
TseI 328 GCAGC G^C[AT]GC
Tsp45I 343 GTCAC ^GT[GC]AC
TthHB8I 210 TCGA T^CGA
XbaI 321 TCTAGA T^CTAGA
XhoII 275 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AccI 172 GTCTAC GT^[AC][GT]AC
412 GTATAC
AfaI 214 GTAC GT^AC
460 GTAC
BfaI 322 CTAG C^TAG
430 CTAG
BsrI 53 CCAGT ACTGG(1/-1)
417 CCAGT
Cfr13I 100 GGACC G^G[ACGT]CC
467 GGCCC
HaeIII 156 GGCC GG^CC
467 GGCC
MaeI 322 CTAG C^TAG
430 CTAG
MaeII 20 ACGT A^CGT
346 ACGT
MnlI 103 CCTC CCTC(7/6)
470 CCTC
RsaI 214 GTAC GT^AC
460 GTAC
Sau96I 100 GGACC G^G[ACGT]CC
467 GGCCC
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# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AcsI AluI ApoI BsaJI DdeI
HinfI MboII MseI NlaIII TfiI
Tru9I Tsp509I TspRI