################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AflII AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaBI BsaI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 828 GTCTAC GT^[AC][GT]AC AcyI 1492 GACGCC G[GA]^CG[CT]C AfaI 1156 GTAC GT^AC AflIII 641 ACGTGT A^C[GA][CT]GT AgeI 1108 ACCGGT A^CCGGT AvaI 734 CCCGGG C^[CT]CG[GA]G AvaII 1093 GGTCC G^G[AT]CC BamHI 216 GGATCC G^GATCC BbsI 838 GAAGAC GAAGAC(2/6) BpuAI 838 GAAGAC GAAGAC(2/6) BsaAI 487 TACGTA [CT]AC^GT[GA] BsaHI 1492 GACGCC G[GA]^CG[CT]C BsaWI 1108 ACCGGT [AT]^CCGG[AT] BseRI 1016 CTCCTC GAGGAG(10/8) BsiYI 1549 CCTGGAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 1549 CCTGGAATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 377 GTCCC GGGAC(10/14) BsoBI 734 CCCGGG C^[CT]CG[GA]G BsrDI 1088 GCAATG GCAATG(2/0) BstYI 216 GGATCC [GA]^GATC[CT] Cfr13I 1093 GGTCC G^G[ACGT]CC DraIII 1453 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 1048 GATATC GAT^ATC HgaI 1492 GACGC GACGC(5/10) Hin1I 1492 GACGCC G[GA]^CG[CT]C HindIII 45 AAGCTT A^AGCTT MflI 216 GGATCC [GA]^GATC[CT] MslI 175 CACCTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 685 CATATG CA^TATG NspI 892 GCATGT [GA]CATG^[CT] PinAI 1108 ACCGGT A^CCGGT RsaI 1156 GTAC GT^AC Sau96I 1093 GGTCC G^G[ACGT]CC SmaI 734 CCCGGG CCC^GGG SmlI 1159 CTTGAG C^T[CT][GA]AG SnaBI 487 TACGTA TAC^GTA StuI 34 AGGCCT AGG^CCT Tsp45I 303 GTCAC ^GT[GC]AC XhoII 216 GGATCC [GA]^GATC[CT] XmaCI 734 CCCGGG C^CCGGG XmaI 734 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BalI 333 TGGCCA TGG^CCA 1508 TGGCCA BanI 150 GGTGCC G^G[CT][GA]CC 173 GGCACC BbvI 1482 GCAGC GCAGC(8/12) 1085 GCTGC BcnI 734 CCCGG CC^[GC]GG 735 CCGGG BsgI 1527 CTGCAC GTGCAG(16/14) 1559 GTGCAG BspMI 114 ACCTGC ACCTGC(4/8) 225 GCAGGT BsrI 1237 ACTGG ACTGG(1/-1) 269 CCAGT BstAPI 109 GCACCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 979 GCAGATGATGC Cac8I 127 GCCTGC GC[ACGT]^[ACGT]GC 1357 GCAGGC EaeI 333 TGGCCA [CT]^GGCC[GA] 1508 TGGCCA Fnu4HI 1085 GCTGC GC^[ACGT]GC 1482 GCAGC ItaI 1085 GCTGC GC^[ACGT]GC 1482 GCAGC MluNI 333 TGGCCA TGG^CCA 1508 TGGCCA MscI 333 TGGCCA TGG^CCA 1508 TGGCCA MspA1I 943 CAGCTG C[AC]G^C[GT]G 1483 CAGCTG NciI 734 CCCGG CC^[GC]GG 735 CCGGG PstI 977 CTGCAG CTGCA^G 1021 CTGCAG PvuII 943 CAGCTG CAG^CTG 1483 CAGCTG TseI 1085 GCTGC G^C[AT]GC 1482 GCAGC TspRI 270 CAGTG CA[GC]TG[ACGT][ACGT]^ 1562 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AcsI AluI AlwI AlwNI ApoI BfaI BsaJI BsmI BsrFI BssKI BstF5I BstNI Cfr10I DdeI DpnII DraI EarI EcoRII FokI HaeIII HapII HinfI HpaII HphI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI SfcI TfiI Tru9I Tsp509I