################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BbeI BbrPI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FseI FspI HaeII HgaI HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1060 GACGTC GACGT^C AcsI 744 AAATTC [GA]^AATT[CT] AcyI 1060 GACGTC G[GA]^CG[CT]C AlwNI 1354 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 1698 GGGCCC GGGCC^C ApoI 744 AAATTC [GA]^AATT[CT] AseI 147 ATTAAT AT^TAAT AsnI 147 ATTAAT AT^TAAT AvaII 675 GGACC G^G[AT]CC BamHI 894 GGATCC G^GATCC BanI 86 GGCACC G^G[CT][GA]CC BbvCI 160 CCTCAGC CCTCAGC(-5/-2) BcgI 552 CGACTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1343 TGATCA T^GATCA BglII 584 AGATCT A^GATCT BsaBI 763 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1060 GACGTC G[GA]^CG[CT]C BsaI 347 GAGACC GGTCTC(1/5) BsaWI 1394 ACCGGA [AT]^CCGG[AT] BsgI 1369 CTGCAC GTGCAG(16/14) BspMI 1367 ACCTGC ACCTGC(4/8) BstBI 1766 TTCGAA TT^CGAA BstXI 891 CCAGGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 1422 GCTAGC GC[ACGT]^[ACGT]GC DraI 1459 TTTAAA TTT^AAA EcoRV 1576 GATATC GAT^ATC EcoT14I 839 CCTTGG C^C[AT][AT]GG EcoT22I 691 ATGCAT ATGCA^T FauI 1217 CCCGC CCCGC(4/6) FbaI 1343 TGATCA T^GATCA Hin1I 1060 GACGTC G[GA]^CG[CT]C MamI 763 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 529 CATACCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NheI 1422 GCTAGC G^CTAGC NsiI 691 ATGCAT ATGCA^T NspI 689 ACATGC [GA]CATG^[CT] NspV 1766 TTCGAA TT^CGAA PshAI 779 GACAACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 147 ATTAAT AT^TAAT PstI 1052 CTGCAG CTGCA^G SapI 568 GCTCTTC GCTCTTC(1/4) ScaI 1529 AGTACT AGT^ACT SfaNI 1759 GATGC GCATC(5/9) SfcI 1052 CTGCAG C^T[GA][CT]AG SfuI 1766 TTCGAA TT^CGAA SspI 713 AATATT AAT^ATT StuI 1638 AGGCCT AGG^CCT StyI 839 CCTTGG C^C[AT][AT]GG XcmI 1608 CCAACAGGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 54 GTAC GT^AC 1530 GTAC Asp700I 565 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 662 GAAATGATTC BalI 644 TGGCCA TGG^CCA 1650 TGGCCA BanII 463 GAGCCC G[GA]GC[CT]^C 1698 GGGCCC BbvI 1356 GCAGC GCAGC(8/12) 1589 GCAGC BfaI 1423 CTAG C^TAG 1586 CTAG BstYI 584 AGATCT [GA]^GATC[CT] 894 GGATCC EaeI 644 TGGCCA [CT]^GGCC[GA] 1650 TGGCCA Fnu4HI 1356 GCAGC GC^[ACGT]GC 1589 GCAGC HapII 1395 CCGG C^CGG 1689 CCGG HpaII 1395 CCGG C^CGG 1689 CCGG ItaI 1356 GCAGC GC^[ACGT]GC 1589 GCAGC MaeI 1423 CTAG C^TAG 1586 CTAG MaeII 491 ACGT A^CGT 1061 ACGT MflI 584 AGATCT [GA]^GATC[CT] 894 GGATCC MluNI 644 TGGCCA TGG^CCA 1650 TGGCCA MscI 644 TGGCCA TGG^CCA 1650 TGGCCA MspA1I 224 CAGCTG C[AC]G^C[GT]G 1357 CAGCTG MspI 1395 CCGG C^CGG 1689 CCGG PvuII 224 CAGCTG CAG^CTG 1357 CAGCTG RsaI 54 GTAC GT^AC 1530 GTAC TfiI 667 GATTC G^A[AT]TC 811 GATTC TseI 1356 GCAGC G^C[AT]GC 1589 GCAGC Tsp509I 146 AATT ^AATT 745 AATT XhoII 584 AGATCT [GA]^GATC[CT] 894 GGATCC XmnI 565 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 662 GAAATGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AluI AlwI AocI BbsI BmyI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Bsu36I Cfr13I DdeI DpnII DraII EarI Eco81I EcoO109I EcoRII FokI HaeIII HinfI HphI Ksp632I MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI TaqI Tru9I Tsp45I TspRI TthHB8I