Restriction map for
pFN21AE5773
(Circular) (Six-base) MAPSORT of: pFN21AE5773.seq Check: 7991 from: 1 to: 6508
pFN21AE5773 6508 bp
With 182 enzymes: SgfI *
December 8, 2014 13:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3069 4318 278
Size: 53 83 186 936 1533 1249 2468
Fragments arranged by size:
2468 1533 1249 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3851 1937
Size: 1914 4594
Acc65I G'GTAC_C
Cuts at: 3829 3829
Size: 6508
AclI AA'CG_TT
Cuts at: 1563 4635 5008 1563
Size: 3072 373 3063
Fragments arranged by size:
3072 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2748 3708 4571 5955 6223 1632
Size: 1116 960 863 1384 268 1917
Fragments arranged by size:
1917 1384 1116 960 863 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6311
AflIII A'CryG_T
Cuts at: 1203 5407 6384 1203
Size: 4204 977 1327
Fragments arranged by size:
4204 1327 977
AgeI A'CCGG_T
Cuts at: 5339 5339
Size: 6508
AhdI GACnn_n'nnGTC
Cuts at: 5237 5237
Size: 6508
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6508
AlwNI CAG_nnn'CTG
Cuts at: 3364 4251 5823 3364
Size: 887 1572 4049
Fragments arranged by size:
4049 1572 887
ApaI G_GGCC'C
Cuts at: 3707 3707
Size: 6508
ApaLI G'TGCA_C
Cuts at: 4565 5721 4565
Size: 1156 5352
ApoI r'AATT_y
Cuts at: 1493 2749 3817 4043 4195 6304 1493
Size: 1256 1068 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 1068 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2153 5062 160
Size: 872 1121 2909 1606
Fragments arranged by size:
2909 1606 1121 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
AvaI C'yCGr_G
Cuts at: 1870 1943 3833 1870
Size: 73 1890 4545
Fragments arranged by size:
4545 1890 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2485 2518 2485
Size: 33 6475
BamHI G'GATC_C
Cuts at: 2899 3838 4205 2899
Size: 939 367 5202
Fragments arranged by size:
5202 939 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 3829 5284 618
Size: 325 217 627 304 1738 1455 1842
Fragments arranged by size:
1842 1738 1455 627 325 304 217
BanII G_rGCy'C
Cuts at: 1391 2472 3707 3827 1391
Size: 1081 1235 120 4072
Fragments arranged by size:
4072 1235 1081 120
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2754 3069 3282 928
Size: 971 855 315 213 4154
Fragments arranged by size:
4154 971 855 315 213
BbvCI CC'TCA_GC
Cuts at: 2166 2166
Size: 6508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2546 2580 4699 4733 1903
Size: 34 609 34 2119 34 3678
Fragments arranged by size:
3678 2119 609 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4400 5610 4400
Size: 1210 5298
BclI T'GATC_A
Cuts at: 1548 3348 1548
Size: 1800 4708
BfrBI ATG'CAT
Cuts at: 2698 2698
Size: 6508
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5119 136
Size: 107 122 71 631 605 3447 1525
Fragments arranged by size:
3447 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2589 6503 2589
Size: 3914 2594
BlpI GC'TnA_GC
Cuts at: 3925 3925
Size: 6508
Bme1580I G_kGCm'C
Cuts at: 1790 2094 2895 3707 4569 5725 1790
Size: 304 801 812 862 1156 2573
Fragments arranged by size:
2573 1156 862 812 801 304
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6508
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3702 4239 5197
Size: 416 53 83 399 24 2285 537 958
Cuts at: 5197 442
Size: 1753
Fragments arranged by size:
2285 1753 958 537 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3431 1056
Size: 2375 4133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3132 3132
Size: 6508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2397 2486 3482 3498 3523 3649 5168
Size: 812 346 89 996 16 25 126 1519
Cuts at: 5168 1239
Size: 2579
Fragments arranged by size:
2579 1519 996 812 346 126 89 25
16
Bpu10I CC'TnA_GC
Cuts at: 2166 2281 2166
Size: 115 6393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4633 5498 5796 4633
Size: 865 298 5345
Fragments arranged by size:
5345 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2346 5171 882
Size: 617 847 2825 2219
Fragments arranged by size:
2825 2219 847 617
BsaAI yAC'GTr
Cuts at: 493 1204 4299 493
Size: 711 3095 2702
Fragments arranged by size:
3095 2702 711
BsaBI GATnn'nnATC
Cuts at: 2772 6502 2772
Size: 3730 2778
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3066 4315 4697 5332
Size: 53 83 186 936 1533 1249 382 635
Cuts at: 5332 275
Size: 1451
Fragments arranged by size:
1533 1451 1249 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3399 4941 5339 5613 5760 6171 3399
Size: 1542 398 274 147 411 3736
Fragments arranged by size:
3736 1542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2357 2387 2543 2573 2725
Size: 30 165 30 954 30 156 30 152
Cuts at: 2725 2755 3594 3624 1178
Size: 30 839 30 4062
Fragments arranged by size:
4062 954 839 165 156 152 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3027 3180 3197 3209 3263 3695 1186
Size: 1841 153 17 12 54 432 3999
Fragments arranged by size:
3999 1841 432 153 54 17 12
BseYI C'CCAG_C
Cuts at: 2874 5711 6207 2874
Size: 2837 496 3175
Fragments arranged by size:
3175 2837 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3359 1163
Size: 573 1623 4312
Fragments arranged by size:
4312 1623 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3958 4719 5747 1540
Size: 275 186 1957 761 1028 2301
Fragments arranged by size:
2301 1957 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4569 4654 5725 4569
Size: 85 1071 5352
Fragments arranged by size:
5352 1071 85
BsmI GAATG_Cn'
Cuts at: 4019 4112 4019
Size: 93 6415
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2094 2472 2895 3707 3827 4569 4654
Size: 399 304 378 423 812 120 742 85
Cuts at: 4654 5725 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 812 742 423 399 378 304
120 85
BspEI T'CCGG_A
Cuts at: 6171 6171
Size: 6508
BspHI T'CATG_A
Cuts at: 4397 6127 4397
Size: 1730 4778
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3381 3863 844
Size: 2537 482 3489
Fragments arranged by size:
3489 2537 482
BsrBI CCG'CTC
Cuts at: 1394 1823 4395 1394
Size: 429 2572 3507
Fragments arranged by size:
3507 2572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5003 5177 65
Size: 1183 3755 174 1396
Fragments arranged by size:
3755 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5152 5339 1700
Size: 110 3342 187 2869
Fragments arranged by size:
3342 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6508
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6508
BssSI C'ACGA_G
Cuts at: 4568 5580 4568
Size: 1012 5496
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6508
BstBI TT'CG_AA
Cuts at: 3772 5312 3772
Size: 1540 4968
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6508
BstXI CCAn_nnnn'nTGG
Cuts at: 2903 2903
Size: 6508
BstYI r'GATC_y
Cuts at: 1972 2589 2899 3838 4205 4590 4607 6059 6145
Size: 617 310 939 367 385 17 1452 86
Cuts at: 6145 6157 6503 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 939 617 385 367 346 310
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2445 2913 3721 2445
Size: 468 808 5232
Fragments arranged by size:
5232 808 468
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5956
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5769
Fragments arranged by size:
5769 683 56
BtsI GCAGTG_nn'
Cuts at: 4024 4818 4838 4024
Size: 794 20 5694
Fragments arranged by size:
5694 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6508
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6473
DraI TTT'AAA
Cuts at: 3466 3812 3466
Size: 346 6162
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5515 809
Size: 507 4199 1802
Fragments arranged by size:
4199 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2649 3655 3955
Size: 54 1425 50 275 18 819 1006 300
Cuts at: 3955 4844 6473 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1006 889 819 300 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3955 1537
Size: 275 2143 4090
Fragments arranged by size:
4090 2143 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2249 2344 2580 2916 3099 3186 3466 3769
Size: 896 95 236 336 183 87 280 303
Cuts at: 3769 4438 1353
Size: 669 3423
Fragments arranged by size:
3423 896 669 336 303 280 236 183
95 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2468 2598 5095 5469 5615 387
Size: 1064 138 879 130 2497 374 146 1280
Fragments arranged by size:
2497 1280 1064 879 374 146 138 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2397 2486 2748 3482 3498 3523
Size: 393 419 346 89 262 734 16 25
Cuts at: 3523 3649 3708 4571 5168 5955 6223 1239
Size: 126 59 863 597 787 268 1524
Fragments arranged by size:
1524 863 787 734 597 419 393 346
268 262 126 89 59 25 16
EcoO109I rG'GnC_Cy
Cuts at: 2522 3052 3703 3704 2522
Size: 530 651 1 5326
Fragments arranged by size:
5326 651 530 1
EcoRI G'AATT_C
Cuts at: 3817 4195 3817
Size: 378 6130
EcoRV GAT'ATC
Cuts at: 3583 3583
Size: 6508
FspI TGC'GCA
Cuts at: 5014 5014
Size: 6508
HaeII r_GCGC'y
Cuts at: 1678 5655 1678
Size: 3977 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2024 2056 2351 2357 2383
Size: 32 135 32 452 32 295 6 26
Cuts at: 2383 2389 2543 2575 3594 3626 5149 5181 5223
Size: 6 154 32 1019 32 1523 32 42
Cuts at: 5223 5255 1373
Size: 32 2626
Fragments arranged by size:
2626 1523 1019 452 295 154 135 42
32 32 32 32 32 32 32 26
6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3852 4098 669
Size: 988 281 1914 246 3079
Fragments arranged by size:
3079 1914 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6508
HpaI GTT'AAC
Cuts at: 4098 4098
Size: 6508
KpnI G_GTAC'C
Cuts at: 3833 3833
Size: 6508
MfeI C'AATT_G
Cuts at: 4107 4107
Size: 6508
MluI A'CGCG_T
Cuts at: 6384 6384
Size: 6508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3637 3694 3771 5622 5806 1305
Size: 2332 57 77 1851 184 2007
Fragments arranged by size:
2332 2007 1851 184 77 57
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2651 3657 10
Size: 54 1425 343 819 1006 2861
Fragments arranged by size:
2861 1425 1006 819 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2538 4466 4825 4984 518
Size: 648 1372 1928 359 159 2042
Fragments arranged by size:
2042 1928 1372 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2231 3364 3924 4601 5749 5994 1606
Size: 625 1133 560 677 1148 245 2120
Fragments arranged by size:
2120 1148 1133 677 625 560 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5956
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6508
NheI G'CTAG_C
Cuts at: 1052 3427 1052
Size: 2375 4133
NotI GC'GGCC_GC
Cuts at: 3955 3955
Size: 6508
NsiI A_TGCA'T
Cuts at: 2700 2700
Size: 6508
NspI r_CATG'y
Cuts at: 1132 1229 2698 3866 5411 1132
Size: 97 1469 1168 1545 2229
Fragments arranged by size:
2229 1545 1469 1168 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6508
PciI A'CATG_T
Cuts at: 5407 5407
Size: 6508
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6508
PfoI T'CCnGG_A
Cuts at: 2010 2902 3661 2010
Size: 892 759 4857
Fragments arranged by size:
4857 892 759
PmeI GTTT'AAAC
Cuts at: 3812 3812
Size: 6508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3753 3785 4577 4609 6090 6122 3753
Size: 32 792 32 1481 32 4139
Fragments arranged by size:
4139 1481 792 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2788 1613
Size: 1175 5333
PsiI TTA'TAA
Cuts at: 4078 4078
Size: 6508
PspOMI G'GGCC_C
Cuts at: 3703 3703
Size: 6508
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2416 2448 2416
Size: 32 6476
PstI C_TGCA'G
Cuts at: 830 3061 3860 830
Size: 2231 799 3478
Fragments arranged by size:
3478 2231 799
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6508
PvuII CAG'CTG
Cuts at: 2231 3364 2231
Size: 1133 5375
SalI G'TCGA_C
Cuts at: 1936 3850 1936
Size: 1914 4594
SapI GCTCTTCn'nnn_
Cuts at: 2580 2580
Size: 6508
SbfI CC_TGCA'GG
Cuts at: 3860 3860
Size: 6508
ScaI AGT'ACT
Cuts at: 3536 4756 6468 3536
Size: 1220 1712 3576
Fragments arranged by size:
3576 1712 1220
SfcI C'TryA_G
Cuts at: 826 1045 3057 3856 4991 5672 5863 826
Size: 219 2012 799 1135 681 191 1471
Fragments arranged by size:
2012 1471 1135 799 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6508
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6508
SmaI CCC'GGG
Cuts at: 1872 3835 1872
Size: 1963 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4612 5513 5775 820
Size: 197 926 2669 901 262 1553
Fragments arranged by size:
2669 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4299 493
Size: 3806 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6508
SphI G_CATG'C
Cuts at: 1132 3866 1132
Size: 2734 3774
SspI AAT'ATT
Cuts at: 5 52 2720 4432 5
Size: 47 2668 1712 2081
Fragments arranged by size:
2668 2081 1712 47
StuI AGG'CCT
Cuts at: 1517 3645 1517
Size: 2128 4380
StyI C'CwwG_G
Cuts at: 513 1065 2844 3947 513
Size: 552 1779 1103 3074
Fragments arranged by size:
3074 1779 1103 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2370 3143 4535 4552 4705 4890 5335
Size: 1255 790 773 1392 17 153 185 445
Cuts at: 5335 325
Size: 1498
Fragments arranged by size:
1498 1392 1255 790 773 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3534 4754 6466
Size: 274 80 33 51 1443 1557 1220 1712
Cuts at: 6466 96
Size: 138
Fragments arranged by size:
1712 1557 1443 1220 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 2725 2757 6468 6500 2084
Size: 32 609 32 3711 32 2092
Fragments arranged by size:
3711 2092 609 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6508
XbaI T'CTAG_A
Cuts at: 3844 3844
Size: 6508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3620 3620
Size: 6508
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6508
XmaI C'CCGG_G
Cuts at: 1870 3833 1870
Size: 1963 4545
XmnI GAAnn'nnTTC
Cuts at: 2574 2671 4637 2574
Size: 97 1966 4445
Fragments arranged by size:
4445 1966 97
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3067 4316 276
Size: 53 83 186 936 1533 1249 2468
Fragments arranged by size:
2468 1533 1249 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BsiWI BsmBI
BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI
RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI
SwaI