Restriction map for
pFN21AE5773
(Linear) (Six-base) MAPSORT of: KIEE5773.seq Check: 9904 from: 1 to: 1803
KIEE5773.orf
With 182 enzymes: SgfI *
December 5, 2014 13:10 ..
AatII G_ACGT'C
Cuts at: 0 1064 1803
Size: 1064 739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1703 1803
Size: 743 960 100
Fragments arranged by size:
960 743 100
AlwNI CAG_nnn'CTG
Cuts at: 0 1359 1803
Size: 1359 444
ApaI G_GGCC'C
Cuts at: 0 1702 1803
Size: 1702 101
ApoI r'AATT_y
Cuts at: 0 744 1803
Size: 744 1059
AseI AT'TA_AT
Cuts at: 0 148 1803
Size: 148 1655
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 480 513 1803
Size: 480 33 1290
Fragments arranged by size:
1290 480 33
BamHI G'GATC_C
Cuts at: 0 894 1803
Size: 894 909
BanI G'GyrC_C
Cuts at: 0 86 1803
Size: 86 1717
BanII G_rGCy'C
Cuts at: 0 467 1702 1803
Size: 467 1235 101
Fragments arranged by size:
1235 467 101
BbsI GAAGACnn'nnnn_
Cuts at: 0 749 1064 1277 1803
Size: 749 315 213 526
Fragments arranged by size:
749 526 315 213
BbvCI CC'TCA_GC
Cuts at: 0 161 1803
Size: 161 1642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 575 1803
Size: 541 34 1228
Fragments arranged by size:
1228 541 34
BclI T'GATC_A
Cuts at: 0 1343 1803
Size: 1343 460
BfrBI ATG'CAT
Cuts at: 0 693 1803
Size: 693 1110
BglII A'GATC_T
Cuts at: 0 584 1803
Size: 584 1219
Bme1580I G_kGCm'C
Cuts at: 0 89 890 1702 1803
Size: 89 801 812 101
Fragments arranged by size:
812 801 101 89
BmrI ACTGGGnnnn_n'
Cuts at: 0 1697 1803
Size: 1697 106
BmtI G_CTAG'C
Cuts at: 0 1426 1803
Size: 1426 377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1127 1803
Size: 1127 676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 392 481 1477 1493 1518 1644 1803
Size: 46 346 89 996 16 25 126 159
Fragments arranged by size:
996 346 159 126 89 46 25 16
Bpu10I CC'TnA_GC
Cuts at: 0 161 276 1803
Size: 161 115 1527
Fragments arranged by size:
1527 161 115
BsaI GGTCTCn'nnnn_
Cuts at: 0 341 1803
Size: 341 1462
BsaBI GATnn'nnATC
Cuts at: 0 767 1803
Size: 767 1036
BsaHI Gr'CG_yC
Cuts at: 0 1061 1803
Size: 1061 742
BsaWI w'CCGG_w
Cuts at: 0 1394 1803
Size: 1394 409
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 352 382 538 568 720 750 1589 1619
Size: 352 30 156 30 152 30 839 30
Cuts at: 1619 1803
Size: 184
Fragments arranged by size:
839 352 184 156 152 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1022 1175 1192 1204 1258 1690 1803
Size: 1022 153 17 12 54 432 113
Fragments arranged by size:
1022 432 153 113 54 17 12
BseYI C'CCAG_C
Cuts at: 0 869 1803
Size: 869 934
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1354 1803
Size: 1354 449
Bsp1286I G_dGCh'C
Cuts at: 0 89 467 890 1702 1803
Size: 89 378 423 812 101
Fragments arranged by size:
812 423 378 101 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1376 1803
Size: 1376 427
BstBI TT'CG_AA
Cuts at: 0 1767 1803
Size: 1767 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 898 1803
Size: 898 905
BstYI r'GATC_y
Cuts at: 0 584 894 1803
Size: 584 310 909
Fragments arranged by size:
909 584 310
Bsu36I CC'TnA_GG
Cuts at: 0 440 908 1716 1803
Size: 440 468 808 87
Fragments arranged by size:
808 468 440 87
DraI TTT'AAA
Cuts at: 0 1461 1803
Size: 1461 342
EaeI y'GGCC_r
Cuts at: 0 644 1650 1803
Size: 644 1006 153
Fragments arranged by size:
1006 644 153
EarI CTCTTCn'nnn_
Cuts at: 0 244 339 575 911 1094 1181 1461 1764
Size: 244 95 236 336 183 87 280 303
Cuts at: 1764 1803
Size: 39
Fragments arranged by size:
336 303 280 244 236 183 95 87
39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 463 593 1803
Size: 463 130 1210
Fragments arranged by size:
1210 463 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 392 481 743 1477 1493 1518 1644
Size: 46 346 89 262 734 16 25 126
Cuts at: 1644 1703 1803
Size: 59 100
Fragments arranged by size:
734 346 262 126 100 89 59 46
25 16
EcoO109I rG'GnC_Cy
Cuts at: 0 517 1047 1698 1699 1803
Size: 517 530 651 1 104
Fragments arranged by size:
651 530 517 104 1
EcoRV GAT'ATC
Cuts at: 0 1578 1803
Size: 1578 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 346 352 378 384 538 570
Size: 19 32 295 6 26 6 154 32
Cuts at: 570 1589 1621 1803
Size: 1019 32 182
Fragments arranged by size:
1019 295 182 154 32 32 32 26
19 6 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1632 1689 1766 1803
Size: 1632 57 77 37
Fragments arranged by size:
1632 77 57 37
MscI TGG'CCA
Cuts at: 0 646 1652 1803
Size: 646 1006 151
Fragments arranged by size:
1006 646 151
MslI CAynn'nnrTG
Cuts at: 0 533 1803
Size: 533 1270
MspA1I CmG'CkG
Cuts at: 0 226 1359 1803
Size: 226 1133 444
Fragments arranged by size:
1133 444 226
NheI G'CTAG_C
Cuts at: 0 1422 1803
Size: 1422 381
NsiI A_TGCA'T
Cuts at: 0 695 1803
Size: 695 1108
NspI r_CATG'y
Cuts at: 0 693 1803
Size: 693 1110
PfoI T'CCnGG_A
Cuts at: 0 5 897 1656 1803
Size: 5 892 759 147
Fragments arranged by size:
892 759 147 5
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1748 1780 1803
Size: 1748 32 23
Fragments arranged by size:
1748 32 23
PshAI GACnn'nnGTC
Cuts at: 0 783 1803
Size: 783 1020
PspOMI G'GGCC_C
Cuts at: 0 1698 1803
Size: 1698 105
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 411 443 1803
Size: 411 32 1360
Fragments arranged by size:
1360 411 32
PstI C_TGCA'G
Cuts at: 0 1056 1803
Size: 1056 747
PvuII CAG'CTG
Cuts at: 0 226 1359 1803
Size: 226 1133 444
Fragments arranged by size:
1133 444 226
SapI GCTCTTCn'nnn_
Cuts at: 0 575 1803
Size: 575 1228
ScaI AGT'ACT
Cuts at: 0 1531 1803
Size: 1531 272
SfcI C'TryA_G
Cuts at: 0 1052 1803
Size: 1052 751
SspI AAT'ATT
Cuts at: 0 715 1803
Size: 715 1088
StuI AGG'CCT
Cuts at: 0 1640 1803
Size: 1640 163
StyI C'CwwG_G
Cuts at: 0 839 1803
Size: 839 964
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 365 1138 1803
Size: 365 773 665
Fragments arranged by size:
773 665 365
TatI w'GTAC_w
Cuts at: 0 1529 1803
Size: 1529 274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 720 752 1803
Size: 79 32 609 32 1051
Fragments arranged by size:
1051 609 79 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1615 1803
Size: 1615 188
XmnI GAAnn'nnTTC
Cuts at: 0 569 666 1803
Size: 569 97 1137
Fragments arranged by size:
1137 569 97
ZraI GAC'GTC
Cuts at: 0 1062 1803
Size: 1062 741
Enzymes that do cut:
AatII AcuI AlwNI ApaI ApoI AseI BaeI BamHI
BanI BanII BbsI BbvCI BcgI BclI BfrBI BglII
Bme1580I BmrI BmtI BplI BpmI Bpu10I BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI Bsp1286I BspMI
BstBI BstXI BstYI Bsu36I DraI EaeI EarI EciI
Eco57MI EcoO109I EcoRV Hin4I MmeI MscI MslI MspA1I
NheI NsiI NspI PfoI PpiI PshAI PspOMI PsrI
PstI PvuII SapI ScaI SfcI SspI StuI StyI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AsiSI AvaI
AvrII BbeI BciVI BglI BlpI BmgBI BpuEI BsaAI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraIII DrdI EagI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NcoI NdeI NgoMIV NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpuMI PsiI PspXI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI