Restriction map for
pFN21AE6785
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccII AclI AcsI AcyI
AflII AgeI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BbrPI
BcnI BfrI BlnI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoT14I EcoT22I FseI FspI
HaeII Hin1I HindIII HpaI KasI
KspI MamI MfeI MluI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StyI SwaI Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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Acc65I 213 GGTACC G^GTACC
AccI 626 GTCTAC GT^[AC][GT]AC
AflIII 1115 ACATGT A^C[GA][CT]GT
Alw44I 55 GTGCAC G^TGCAC
AlwNI 1429 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 908 GGGCCC GGGCC^C
ApaLI 55 GTGCAC G^TGCAC
Asp718I 213 GGTACC G^GTACC
BamHI 329 GGATCC G^GATCC
BbsI 961 GAAGAC GAAGAC(2/6)
BbvCI 648 CCTCAGC CCTCAGC(-5/-2)
BcgI 597 GCAGCGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 256 GGATAC GTATCC(6/5)
BglI 382 GCCCAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1164 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1164 GCTCAGC GC^T[ACGT]AGC
BpuAI 961 GAAGAC GAAGAC(2/6)
BsaI 361 GAGACC GGTCTC(1/5)
BspHI 1157 TCATGA T^CATGA
BspLU11I 1115 ACATGT A^CATGT
BsrBI 465 CCGCTC CCGCTC(-3/-3)
BsrDI 1088 CATTGC GCAATG(2/0)
BssSI 346 CACGAG CACGAG(-5/-1)
BstAPI 714 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 1164 GCTCAGC GC^T[ACGT]AGC
EcoRV 460 GATATC GAT^ATC
HgaI 120 GACGC GACGC(5/10)
HincII 496 GTCAAC GT[CT]^[GA]AC
HindII 496 GTCAAC GT[CT]^[GA]AC
KpnI 213 GGTACC GGTAC^C
MaeII 1235 ACGT A^CGT
MseI 1648 TTAA T^TAA
PaeR7I 1133 CTCGAG C^TCGAG
PflMI 809 CCAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1019 GGGACCT [GA]G^G[AT]CC[CT]
RcaI 1157 TCATGA T^CATGA
SacI 166 GAGCTC GAGCT^C
SapI 1794 GCTCTTC GCTCTTC(1/4)
SbfI 300 CCTGCAGG CCTGCA^GG
ScaI 444 AGTACT AGT^ACT
SfiI 381 GGCCCAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 1133 CTCGAG C^T[CT][GA]AG
Sse8387I 300 CCTGCAGG CCTGCA^GG
Tru9I 1648 TTAA T^TAA
Tsp509I 12 AATT ^AATT
Van91I 809 CCAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 805 CCAACCAAACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1133 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 236 TCCGGA T^CCGGA
1277 TCCGGA
AspHI 55 GTGCAC G[AT]GC[AT]^C
166 GAGCTC
AvaI 1133 CTCGAG C^[CT]CG[GA]G
1769 CTCGGG
BalI 389 TGGCCA TGG^CCA
881 TGGCCA
BclI 158 TGATCA T^GATCA
582 TGATCA
BfaI 490 CTAG C^TAG
1444 CTAG
BsaWI 236 TCCGGA [AT]^CCGG[AT]
1277 TCCGGA
BseAI 236 TCCGGA T^CCGGA
1277 TCCGGA
BseRI 349 GAGGAG GAGGAG(10/8)
1045 GAGGAG
BsiHKAI 55 GTGCAC G[AT]GC[AT]^C
166 GAGCTC
BsoBI 1133 CTCGAG C^[CT]CG[GA]G
1769 CTCGGG
BspEI 236 TCCGGA T^CCGGA
1277 TCCGGA
BstXI 611 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
894 CCAGCTGATTGG
CfoI 936 GCGC GCG^C
1842 GCGC
EaeI 389 TGGCCA [CT]^GGCC[GA]
881 TGGCCA
FbaI 158 TGATCA T^GATCA
582 TGATCA
HhaI 936 GCGC GCG^C
1842 GCGC
HinP1I 936 GCGC G^CGC
1842 GCGC
MaeI 490 CTAG C^TAG
1444 CTAG
MluNI 389 TGGCCA TGG^CCA
881 TGGCCA
MroI 236 TCCGGA T^CCGGA
1277 TCCGGA
MscI 389 TGGCCA TGG^CCA
881 TGGCCA
StuI 725 AGGCCT AGG^CCT
1421 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AvaII BanI BanII BbvI BglII
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII EarI Eco81I EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XhoII