(Circular) (Six-base) MAPSORT of: pFN21AE6785.seq Check: 3070 from: 1 to: 6625 pFN21AE6785 6625 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2983 3537 2983 Size: 554 6071 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4435 278 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2632 3968 1937 Size: 695 1336 4594 Fragments arranged by size: 4594 1336 695 Acc65I G'GTAC_C Cuts at: 2218 3946 2218 Size: 1728 4897 AclI AA'CG_TT Cuts at: 1563 4752 5125 1563 Size: 3189 373 3063 Fragments arranged by size: 3189 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3050 3787 4688 6072 6340 1632 Size: 1418 737 901 1384 268 1917 Fragments arranged by size: 1917 1418 1384 901 737 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6428 AflIII A'CryG_T Cuts at: 1203 3120 5524 6501 1203 Size: 1917 2404 977 1327 Fragments arranged by size: 2404 1917 1327 977 AgeI A'CCGG_T Cuts at: 5456 5456 Size: 6625 AhdI GACnn_n'nnGTC Cuts at: 5354 5354 Size: 6625 AleI CACnn'nnGTG Cuts at: 1166 2621 2867 1166 Size: 1455 246 4924 Fragments arranged by size: 4924 1455 246 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2885 3022 3061 1184 Size: 1701 137 39 4748 Fragments arranged by size: 4748 1701 137 39 AlwNI CAG_nnn'CTG Cuts at: 3439 4368 5940 3439 Size: 929 1572 4124 Fragments arranged by size: 4124 1572 929 ApaI G_GGCC'C Cuts at: 2917 2917 Size: 6625 ApaLI G'TGCA_C Cuts at: 2060 4682 5838 2060 Size: 2622 1156 2847 Fragments arranged by size: 2847 2622 1156 ApoI r'AATT_y Cuts at: 1493 3934 4160 4312 6421 1493 Size: 2441 226 152 2109 1697 Fragments arranged by size: 2441 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5179 160 Size: 872 4147 1606 Fragments arranged by size: 4147 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 AvaI C'yCGr_G Cuts at: 1870 1943 3138 3774 3950 1870 Size: 73 1195 636 176 4545 Fragments arranged by size: 4545 1195 636 176 73 BamHI G'GATC_C Cuts at: 2334 3955 4322 2334 Size: 1621 367 4637 Fragments arranged by size: 4637 1621 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2218 2861 3515 3946 Size: 325 217 627 363 68 643 654 431 Cuts at: 3946 5401 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 654 643 627 431 363 325 217 68 BanII G_rGCy'C Cuts at: 1391 2175 2668 2917 3063 3423 3944 1391 Size: 784 493 249 146 360 521 4072 Fragments arranged by size: 4072 784 521 493 360 249 146 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2973 928 Size: 971 1074 4580 Fragments arranged by size: 4580 1074 971 BbvCI CC'TCA_GC Cuts at: 2654 2654 Size: 6625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2591 2625 4816 4850 1903 Size: 34 654 34 2191 34 3678 Fragments arranged by size: 3678 2191 654 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2255 4517 5727 2255 Size: 2262 1210 3153 Fragments arranged by size: 3153 2262 1210 BclI T'GATC_A Cuts at: 1548 2163 2587 1548 Size: 615 424 5586 Fragments arranged by size: 5586 615 424 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2393 5236 136 Size: 107 122 71 631 605 721 2843 1525 Fragments arranged by size: 2843 1525 721 631 605 122 107 71 BglII A'GATC_T Cuts at: 3451 3574 3885 6620 3451 Size: 123 311 2735 3456 Fragments arranged by size: 3456 2735 311 123 BlpI GC'TnA_GC Cuts at: 3170 4042 3170 Size: 872 5753 Bme1580I G_kGCm'C Cuts at: 1790 2064 2153 2864 2917 3414 3520 3723 4686 Size: 274 89 711 53 497 106 203 963 Cuts at: 4686 5842 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 963 711 497 274 203 106 89 53 BmgBI CAC'GTC Cuts at: 1318 3241 1318 Size: 1923 4702 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3401 3747 4356 Size: 416 53 83 399 24 1984 346 609 Cuts at: 4356 5314 442 Size: 958 1753 Fragments arranged by size: 1984 1753 958 609 416 399 346 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6625 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2823 2823 Size: 6625 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2307 2759 3716 5285 1239 Size: 1068 452 957 1569 2579 Fragments arranged by size: 2579 1569 1068 957 452 Bpu10I CC'TnA_GC Cuts at: 2654 2654 Size: 6625 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4750 5615 5913 4750 Size: 865 298 5462 Fragments arranged by size: 5462 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2360 5288 882 Size: 617 861 2928 2219 Fragments arranged by size: 2928 2219 861 617 BsaAI yAC'GTr Cuts at: 493 1204 4416 493 Size: 711 3212 2702 Fragments arranged by size: 3212 2702 711 BsaBI GATnn'nnATC Cuts at: 6619 6619 Size: 6625 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4432 4814 5449 275 Size: 53 83 186 936 2899 382 635 1451 Fragments arranged by size: 2899 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2241 3282 5058 5456 5730 5877 6288 2241 Size: 1041 1776 398 274 147 411 2578 Fragments arranged by size: 2578 1776 1041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2223 2253 1178 Size: 30 165 30 820 30 5550 Fragments arranged by size: 5550 820 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 3065 1186 Size: 1183 696 4746 Fragments arranged by size: 4746 1183 696 BseYI C'CCAG_C Cuts at: 2038 2146 2223 2898 2976 3387 3559 3795 5828 Size: 108 77 675 78 411 172 236 2033 Cuts at: 5828 6324 2038 Size: 496 2339 Fragments arranged by size: 2339 2033 675 496 411 236 172 108 78 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2600 2621 3011 3899 1163 Size: 573 864 21 390 888 3889 Fragments arranged by size: 3889 888 864 573 390 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4075 4836 5864 1540 Size: 275 186 2074 761 1028 2301 Fragments arranged by size: 2301 2074 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 2175 4686 4771 5842 2064 Size: 111 2511 85 1071 2847 Fragments arranged by size: 2847 2511 1071 111 85 BsmI GAATG_Cn' Cuts at: 4136 4229 4136 Size: 93 6532 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2153 2175 2668 2864 2917 3063 Size: 399 274 89 22 493 196 53 146 Cuts at: 3063 3414 3423 3520 3723 3944 4686 4771 5842 Size: 351 9 97 203 221 742 85 1071 Cuts at: 5842 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 493 399 351 274 221 203 196 146 97 89 85 53 22 9 BspEI T'CCGG_A Cuts at: 2241 3282 6288 2241 Size: 1041 3006 2578 Fragments arranged by size: 3006 2578 1041 BspHI T'CATG_A Cuts at: 3162 4514 6244 3162 Size: 1352 1730 3543 Fragments arranged by size: 3543 1730 1352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2299 2983 3537 3913 3980 844 Size: 1455 684 554 376 67 3489 Fragments arranged by size: 3489 1455 684 554 376 67 BsrBI CCG'CTC Cuts at: 1394 1823 2472 4512 1394 Size: 429 649 2040 3507 Fragments arranged by size: 3507 2040 649 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3092 5120 5294 65 Size: 1183 1844 2028 174 1396 Fragments arranged by size: 2028 1844 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5269 5456 1700 Size: 110 3459 187 2869 Fragments arranged by size: 3459 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6625 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6625 BssSI C'ACGA_G Cuts at: 2351 4685 5697 2351 Size: 2334 1012 3279 Fragments arranged by size: 3279 2334 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2725 794 Size: 1931 4694 BstBI TT'CG_AA Cuts at: 5429 5429 Size: 6625 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6625 BstXI CCAn_nnnn'nTGG Cuts at: 2623 2906 2623 Size: 283 6342 BstYI r'GATC_y Cuts at: 1972 2334 3442 3451 3574 3885 3955 4322 4707 Size: 362 1108 9 123 311 70 367 385 Cuts at: 4707 4724 6176 6262 6274 6620 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1108 385 367 362 346 311 123 86 70 17 12 9 Bsu36I CC'TnA_GG Cuts at: 2198 2776 3348 2198 Size: 578 572 5475 Fragments arranged by size: 5475 578 572 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5886 Fragments arranged by size: 5886 683 56 BtsI GCAGTG_nn' Cuts at: 2368 2564 2889 3663 4141 4935 4955 2368 Size: 196 325 774 478 794 20 4038 Fragments arranged by size: 4038 794 774 478 325 196 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6625 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6590 DraI TTT'AAA Cuts at: 3929 3929 Size: 6625 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5632 809 Size: 507 4316 1802 Fragments arranged by size: 4316 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2394 2886 4072 Size: 54 1425 50 275 18 564 492 1186 Cuts at: 4072 4961 6590 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1186 889 564 492 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4072 1537 Size: 275 2260 4090 Fragments arranged by size: 4090 2260 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3072 3284 3806 4555 1353 Size: 1719 212 522 749 3423 Fragments arranged by size: 3423 1719 749 522 212 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3194 5212 5586 5732 387 Size: 1064 138 1605 2018 374 146 1280 Fragments arranged by size: 2018 1605 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2173 2173 Size: 6625 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2307 2759 3050 3716 3787 4688 5285 Size: 393 675 452 291 666 71 901 597 Cuts at: 5285 6072 6340 1239 Size: 787 268 1524 Fragments arranged by size: 1524 901 787 675 666 597 452 393 291 268 71 EcoO109I rG'GnC_Cy Cuts at: 2913 3025 3748 3826 2913 Size: 112 723 78 5712 Fragments arranged by size: 5712 723 112 78 EcoRI G'AATT_C Cuts at: 3934 4312 3934 Size: 378 6247 EcoRV GAT'ATC Cuts at: 2467 2467 Size: 6625 FspI TGC'GCA Cuts at: 5131 5131 Size: 6625 HaeII r_GCGC'y Cuts at: 1678 5772 1678 Size: 4094 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2308 2340 2456 2488 2759 Size: 32 135 32 736 32 116 32 271 Cuts at: 2759 2791 3135 3167 5266 5298 5340 5372 1373 Size: 32 344 32 2099 32 42 32 2626 Fragments arranged by size: 2626 2099 736 344 271 135 116 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2503 3969 4215 669 Size: 988 281 565 1466 246 3079 Fragments arranged by size: 3079 1466 988 565 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6625 HpaI GTT'AAC Cuts at: 4215 4215 Size: 6625 KpnI G_GTAC'C Cuts at: 2222 3950 2222 Size: 1728 4897 MfeI C'AATT_G Cuts at: 4224 4224 Size: 6625 MluI A'CGCG_T Cuts at: 6501 6501 Size: 6625 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2834 5739 5923 1305 Size: 1529 2905 184 2007 Fragments arranged by size: 2905 2007 1529 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2396 2888 10 Size: 54 1425 343 564 492 3747 Fragments arranged by size: 3747 1425 564 492 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2459 2621 2867 4583 4942 5101 518 Size: 648 1293 162 246 1716 359 159 2042 Fragments arranged by size: 2042 1716 1293 648 359 246 162 159 MspA1I CmG'CkG Cuts at: 1606 2227 2480 2605 2902 3256 3607 3736 3742 Size: 621 253 125 297 354 351 129 6 Cuts at: 3742 4041 4718 5866 6111 1606 Size: 299 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 621 354 351 299 297 253 245 129 125 6 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5134 Fragments arranged by size: 5134 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6625 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6625 NotI GC'GGCC_GC Cuts at: 4072 4072 Size: 6625 NspI r_CATG'y Cuts at: 1132 1229 2846 2930 3124 3983 5528 1132 Size: 97 1617 84 194 859 1545 2229 Fragments arranged by size: 2229 1617 1545 859 194 97 84 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6625 PciI A'CATG_T Cuts at: 3120 5524 3120 Size: 2404 4221 PflMI CCAn_nnn'nTGG Cuts at: 1108 2820 1108 Size: 1712 4913 PfoI T'CCnGG_A Cuts at: 3312 3438 3456 3312 Size: 126 18 6481 Fragments arranged by size: 6481 126 18 PmeI GTTT'AAAC Cuts at: 3929 3929 Size: 6625 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2026 2058 4694 4726 6207 6239 2026 Size: 32 2636 32 1481 32 2412 Fragments arranged by size: 2636 2412 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3025 3025 Size: 6625 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6625 PsiI TTA'TAA Cuts at: 4195 4195 Size: 6625 PspOMI G'GGCC_C Cuts at: 2913 2913 Size: 6625 PspXI vC'TCGA_Gb Cuts at: 3138 3138 Size: 6625 PstI C_TGCA'G Cuts at: 830 2194 2310 2479 2730 2797 3114 3252 3741 Size: 1364 116 169 251 67 317 138 489 Cuts at: 3741 3747 3977 830 Size: 6 230 3478 Fragments arranged by size: 3478 1364 489 317 251 230 169 138 116 67 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6625 PvuII CAG'CTG Cuts at: 2227 2480 2902 3607 3736 3742 2227 Size: 253 422 705 129 6 5110 Fragments arranged by size: 5110 705 422 253 129 6 SacI G_AGCT'C Cuts at: 2175 2175 Size: 6625 SalI G'TCGA_C Cuts at: 1936 3967 1936 Size: 2031 4594 SapI GCTCTTCn'nnn_ Cuts at: 3806 3806 Size: 6625 SbfI CC_TGCA'GG Cuts at: 2310 3977 2310 Size: 1667 4958 ScaI AGT'ACT Cuts at: 2451 4873 6585 2451 Size: 2422 1712 2491 Fragments arranged by size: 2491 2422 1712 SfcI C'TryA_G Cuts at: 826 1045 2190 2306 2475 2726 2793 3110 3248 Size: 219 1145 116 169 251 67 317 138 Cuts at: 3248 3737 3743 3973 5108 5789 5980 826 Size: 489 6 230 1135 681 191 1471 Fragments arranged by size: 1471 1145 1135 681 489 317 251 230 219 191 169 138 116 67 6 SfiI GGCCn_nnn'nGGCC Cuts at: 2393 2393 Size: 6625 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6625 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6625 SmaI CCC'GGG Cuts at: 1872 3952 1872 Size: 2080 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3138 4729 5630 5892 820 Size: 197 926 1195 1591 901 262 1553 Fragments arranged by size: 1591 1553 1195 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4416 493 Size: 3923 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6625 SphI G_CATG'C Cuts at: 1132 3983 1132 Size: 2851 3774 SspI AAT'ATT Cuts at: 5 52 4549 5 Size: 47 4497 2081 Fragments arranged by size: 4497 2081 47 StuI AGG'CCT Cuts at: 1517 2732 3428 1517 Size: 1215 696 4714 Fragments arranged by size: 4714 1215 696 StyI C'CwwG_G Cuts at: 513 1065 2004 4064 513 Size: 552 939 2060 3074 Fragments arranged by size: 3074 2060 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2879 3710 3809 4652 4669 4822 5007 Size: 1255 1299 831 99 843 17 153 185 Cuts at: 5007 5452 325 Size: 445 1498 Fragments arranged by size: 1498 1299 1255 843 831 445 185 153 99 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2449 3601 4871 Size: 274 80 33 51 1443 472 1152 1270 Cuts at: 4871 6583 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1270 1152 472 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6585 6617 6585 Size: 32 6593 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6625 XbaI T'CTAG_A Cuts at: 3961 3961 Size: 6625 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2817 2817 Size: 6625 XhoI C'TCGA_G Cuts at: 1943 3138 1943 Size: 1195 5430 XmaI C'CCGG_G Cuts at: 1870 3950 1870 Size: 2080 4545 XmnI GAAnn'nnTTC Cuts at: 4754 4754 Size: 6625 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4433 276 Size: 53 83 186 936 2899 2468 Fragments arranged by size: 2899 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI