Restriction map for
pFN21AE6785
(Linear) (Six-base) MAPSORT of: KIEE6785.seq Check: 3374 from: 1 to: 1920
KIEE6785.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 978 1532 1920
Size: 978 554 388
Fragments arranged by size:
978 554 388
AccI GT'mk_AC
Cuts at: 0 627 1920
Size: 627 1293
Acc65I G'GTAC_C
Cuts at: 0 213 1920
Size: 213 1707
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1782 1920
Size: 1045 737 138
Fragments arranged by size:
1045 737 138
AflIII A'CryG_T
Cuts at: 0 1115 1920
Size: 1115 805
AleI CACnn'nnGTG
Cuts at: 0 616 862 1920
Size: 616 246 1058
Fragments arranged by size:
1058 616 246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 880 1017 1056 1920
Size: 880 137 39 864
Fragments arranged by size:
880 864 137 39
AlwNI CAG_nnn'CTG
Cuts at: 0 1434 1920
Size: 1434 486
ApaI G_GGCC'C
Cuts at: 0 912 1920
Size: 912 1008
ApaLI G'TGCA_C
Cuts at: 0 55 1920
Size: 55 1865
AvaI C'yCGr_G
Cuts at: 0 1133 1769 1920
Size: 1133 636 151
Fragments arranged by size:
1133 636 151
BamHI G'GATC_C
Cuts at: 0 329 1920
Size: 329 1591
BanI G'GyrC_C
Cuts at: 0 145 213 856 1510 1920
Size: 145 68 643 654 410
Fragments arranged by size:
654 643 410 145 68
BanII G_rGCy'C
Cuts at: 0 170 663 912 1058 1418 1920
Size: 170 493 249 146 360 502
Fragments arranged by size:
502 493 360 249 170 146
BbsI GAAGACnn'nnnn_
Cuts at: 0 968 1920
Size: 968 952
BbvCI CC'TCA_GC
Cuts at: 0 649 1920
Size: 649 1271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 620 1920
Size: 586 34 1300
Fragments arranged by size:
1300 586 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 250 1920
Size: 250 1670
BclI T'GATC_A
Cuts at: 0 158 582 1920
Size: 158 424 1338
Fragments arranged by size:
1338 424 158
BglI GCCn_nnn'nGGC
Cuts at: 0 388 1920
Size: 388 1532
BglII A'GATC_T
Cuts at: 0 1446 1569 1880 1920
Size: 1446 123 311 40
Fragments arranged by size:
1446 311 123 40
BlpI GC'TnA_GC
Cuts at: 0 1165 1920
Size: 1165 755
Bme1580I G_kGCm'C
Cuts at: 0 59 148 859 912 1409 1515 1718 1920
Size: 59 89 711 53 497 106 203 202
Fragments arranged by size:
711 497 203 202 106 89 59 53
BmgBI CAC'GTC
Cuts at: 0 1236 1920
Size: 1236 684
BmrI ACTGGGnnnn_n'
Cuts at: 0 1396 1742 1920
Size: 1396 346 178
Fragments arranged by size:
1396 346 178
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 818 1920
Size: 818 1102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 754 1711 1920
Size: 302 452 957 209
Fragments arranged by size:
957 452 302 209
Bpu10I CC'TnA_GC
Cuts at: 0 649 1920
Size: 649 1271
BsaI GGTCTCn'nnnn_
Cuts at: 0 355 1920
Size: 355 1565
BsaWI w'CCGG_w
Cuts at: 0 236 1277 1920
Size: 236 1041 643
Fragments arranged by size:
1041 643 236
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 218 248 1920
Size: 218 30 1672
Fragments arranged by size:
1672 218 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 1060 1920
Size: 364 696 860
Fragments arranged by size:
860 696 364
BseYI C'CCAG_C
Cuts at: 0 33 141 218 893 971 1382 1554 1790
Size: 33 108 77 675 78 411 172 236
Cuts at: 1790 1920
Size: 130
Fragments arranged by size:
675 411 236 172 130 108 78 77
33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 616 1006 1894 1920
Size: 595 21 390 888 26
Fragments arranged by size:
888 595 390 26 21
BsiHKAI G_wGCw'C
Cuts at: 0 59 170 1920
Size: 59 111 1750
Fragments arranged by size:
1750 111 59
Bsp1286I G_dGCh'C
Cuts at: 0 59 148 170 663 859 912 1058 1409
Size: 59 89 22 493 196 53 146 351
Cuts at: 1409 1418 1515 1718 1920
Size: 9 97 203 202
Fragments arranged by size:
493 351 203 202 196 146 97 89
59 53 22 9
BspEI T'CCGG_A
Cuts at: 0 236 1277 1920
Size: 236 1041 643
Fragments arranged by size:
1041 643 236
BspHI T'CATG_A
Cuts at: 0 1157 1920
Size: 1157 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 294 978 1532 1920
Size: 294 684 554 388
Fragments arranged by size:
684 554 388 294
BsrBI CCG'CTC
Cuts at: 0 467 1920
Size: 467 1453
BsrDI GCAATG_nn'
Cuts at: 0 1087 1920
Size: 1087 833
BssSI C'ACGA_G
Cuts at: 0 346 1920
Size: 346 1574
BstAPI GCAn_nnn'nTGC
Cuts at: 0 720 1920
Size: 720 1200
BstXI CCAn_nnnn'nTGG
Cuts at: 0 618 901 1920
Size: 618 283 1019
Fragments arranged by size:
1019 618 283
BstYI r'GATC_y
Cuts at: 0 329 1437 1446 1569 1880 1920
Size: 329 1108 9 123 311 40
Fragments arranged by size:
1108 329 311 123 40 9
Bsu36I CC'TnA_GG
Cuts at: 0 193 771 1343 1920
Size: 193 578 572 577
Fragments arranged by size:
578 577 572 193
BtsI GCAGTG_nn'
Cuts at: 0 363 559 884 1658 1920
Size: 363 196 325 774 262
Fragments arranged by size:
774 363 325 262 196
EaeI y'GGCC_r
Cuts at: 0 389 881 1920
Size: 389 492 1039
Fragments arranged by size:
1039 492 389
EarI CTCTTCn'nnn_
Cuts at: 0 1067 1279 1801 1920
Size: 1067 212 522 119
Fragments arranged by size:
1067 522 212 119
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1189 1920
Size: 1189 731
EcoICRI GAG'CTC
Cuts at: 0 168 1920
Size: 168 1752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 754 1045 1711 1782 1920
Size: 302 452 291 666 71 138
Fragments arranged by size:
666 452 302 291 138 71
EcoO109I rG'GnC_Cy
Cuts at: 0 908 1020 1743 1821 1920
Size: 908 112 723 78 99
Fragments arranged by size:
908 723 112 99 78
EcoRV GAT'ATC
Cuts at: 0 462 1920
Size: 462 1458
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 303 335 451 483 754 786 1130 1162
Size: 303 32 116 32 271 32 344 32
Cuts at: 1162 1920
Size: 758
Fragments arranged by size:
758 344 303 271 116 32 32 32
32
HincII GTy'rAC
Cuts at: 0 498 1920
Size: 498 1422
KpnI G_GTAC'C
Cuts at: 0 217 1920
Size: 217 1703
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1920
Size: 829 1091
MscI TGG'CCA
Cuts at: 0 391 883 1920
Size: 391 492 1037
Fragments arranged by size:
1037 492 391
MslI CAynn'nnrTG
Cuts at: 0 454 616 862 1920
Size: 454 162 246 1058
Fragments arranged by size:
1058 454 246 162
MspA1I CmG'CkG
Cuts at: 0 222 475 600 897 1251 1602 1731 1737
Size: 222 253 125 297 354 351 129 6
Cuts at: 1737 1920
Size: 183
Fragments arranged by size:
354 351 297 253 222 183 129 125
6
NspI r_CATG'y
Cuts at: 0 841 925 1119 1920
Size: 841 84 194 801
Fragments arranged by size:
841 801 194 84
PciI A'CATG_T
Cuts at: 0 1115 1920
Size: 1115 805
PflMI CCAn_nnn'nTGG
Cuts at: 0 815 1920
Size: 815 1105
PfoI T'CCnGG_A
Cuts at: 0 1307 1433 1451 1920
Size: 1307 126 18 469
Fragments arranged by size:
1307 469 126 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 21 53 1920
Size: 21 32 1867
Fragments arranged by size:
1867 32 21
PpuMI rG'GwC_Cy
Cuts at: 0 1020 1920
Size: 1020 900
PspOMI G'GGCC_C
Cuts at: 0 908 1920
Size: 908 1012
PspXI vC'TCGA_Gb
Cuts at: 0 1133 1920
Size: 1133 787
PstI C_TGCA'G
Cuts at: 0 189 305 474 725 792 1109 1247 1736
Size: 189 116 169 251 67 317 138 489
Cuts at: 1736 1742 1920
Size: 6 178
Fragments arranged by size:
489 317 251 189 178 169 138 116
67 6
PvuII CAG'CTG
Cuts at: 0 222 475 897 1602 1731 1737 1920
Size: 222 253 422 705 129 6 183
Fragments arranged by size:
705 422 253 222 183 129 6
SacI G_AGCT'C
Cuts at: 0 170 1920
Size: 170 1750
SapI GCTCTTCn'nnn_
Cuts at: 0 1801 1920
Size: 1801 119
SbfI CC_TGCA'GG
Cuts at: 0 305 1920
Size: 305 1615
ScaI AGT'ACT
Cuts at: 0 446 1920
Size: 446 1474
SfcI C'TryA_G
Cuts at: 0 185 301 470 721 788 1105 1243 1732
Size: 185 116 169 251 67 317 138 489
Cuts at: 1732 1738 1920
Size: 6 182
Fragments arranged by size:
489 317 251 185 182 169 138 116
67 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 388 1920
Size: 388 1532
SmlI C'TyrA_G
Cuts at: 0 1133 1920
Size: 1133 787
StuI AGG'CCT
Cuts at: 0 727 1423 1920
Size: 727 696 497
Fragments arranged by size:
727 696 497
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 874 1705 1804 1920
Size: 874 831 99 116
Fragments arranged by size:
874 831 116 99
TatI w'GTAC_w
Cuts at: 0 444 1596 1920
Size: 444 1152 324
Fragments arranged by size:
1152 444 324
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 812 1920
Size: 812 1108
XhoI C'TCGA_G
Cuts at: 0 1133 1920
Size: 1133 787
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV Hin4I HincII
KpnI MmeI MscI MslI MspA1I NspI PciI PflMI
PfoI PpiI PpuMI PspOMI PspXI PstI PvuII SacI
SapI SbfI ScaI SfcI SfiI SmlI StuI TaqII
TatI XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BfrBI BmtI
BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI
BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PmeI PmlI PshAI
PsiI PsrI PvuI RsrII SacII SalI SanDI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StyI SwaI TstI Tth111I XbaI XmaI XmnI
ZraI