Restriction map for
pFN21AE6778
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AvaI AvrII BalI
BanI BbeI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT22I FseI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MamI MluI MluNI MroI MscI
MslI MvnI NarI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AgeI 545 ACCGGT A^CCGGT
AhdI 1321 GACGAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1321 GACGAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1581 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 446 TGCGCA TGC^GCA
BamHI 652 GGATCC G^GATCC
BbrPI 1033 CACGTG CAC^GTG
BbvCI 1417 GCTGAGG CCTCAGC(-5/-2)
BclI 1404 TGATCA T^GATCA
BglII 666 AGATCT A^GATCT
BsiEI 512 CGGTCG CG[GA][CT]^CG
BsmI 1529 GCATTC GAATGC(1/-1)
BsrI 649 ACTGG ACTGG(1/-1)
DraIII 1355 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1321 GACGAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 127 GATATC GAT^ATC
FbaI 1404 TGATCA T^GATCA
FspI 446 TGCGCA TGC^GCA
HincII 23 GTCAAC GT[CT]^[GA]AC
HindII 23 GTCAAC GT[CT]^[GA]AC
MfeI 1232 CAATTG C^AATTG
MunI 1232 CAATTG C^AATTG
NaeI 500 GCCGGC GCC^GGC
NdeI 1538 CATATG CA^TATG
NgoMIV 500 GCCGGC G^CCGGC
PflFI 1581 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 545 ACCGGT A^CCGGT
PmaCI 1033 CACGTG CAC^GTG
PmlI 1033 CACGTG CAC^GTG
PpuMI 473 GGGTCCT [GA]G^G[AT]CC[CT]
SapI 1611 GAAGAGC GCTCTTC(1/4)
SbfI 327 CCTGCAGG CCTGCA^GG
Sse8387I 327 CCTGCAGG CCTGCA^GG
Tth111I 1581 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 770 CCAACGGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 823 GTAC GT^AC
832 GTAC
AlwI 653 GATCC GGATC(4/5)
652 GGATC
BcnI 309 CCGGG CC^[GC]GG
1357 CCCGG
BfaI 1070 CTAG C^TAG
1433 CTAG
BsaAI 1033 CACGTG [CT]AC^GT[GA]
1060 TACGTG
BsaWI 545 ACCGGT [AT]^CCGG[AT]
655 TCCGGT
BsgI 1274 GTGCAG GTGCAG(16/14)
1361 GTGCAG
BspMI 326 ACCTGC ACCTGC(4/8)
1197 GCAGGT
BsrFI 500 GCCGGC [GA]^CCGG[CT]
545 ACCGGT
BstYI 652 GGATCC [GA]^GATC[CT]
666 AGATCT
CfoI 334 GCGC GCG^C
447 GCGC
Cfr10I 500 GCCGGC [GA]^CCGG[CT]
545 ACCGGT
EaeI 278 TGGCCG [CT]^GGCC[GA]
1146 CGGCCA
HhaI 334 GCGC GCG^C
447 GCGC
HinP1I 334 GCGC G^CGC
447 GCGC
MaeI 1070 CTAG C^TAG
1433 CTAG
MflI 652 GGATCC [GA]^GATC[CT]
666 AGATCT
NciI 309 CCGGG CC^[GC]GG
1357 CCCGG
NcoI 630 CCATGG C^CATGG
1346 CCATGG
RsaI 823 GTAC GT^AC
832 GTAC
TaqI 563 TCGA T^CGA
1508 TCGA
TthHB8I 563 TCGA T^CGA
1508 TCGA
XhoII 652 GGATCC [GA]^GATC[CT]
666 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AspHI AvaII BanII
BbsI BbvI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrBI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI