(Circular) (Six-base) MAPSORT of: pFN21AE6778.seq Check: 1067 from: 1 to: 6325 pFN21AE6778 6325 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2340 3193 2340 Size: 853 5472 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3668 1937 Size: 1731 4594 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2555 3318 4388 5772 6040 1632 Size: 455 468 763 1070 1384 268 1917 Fragments arranged by size: 1917 1384 1070 763 468 455 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6128 AflIII A'CryG_T Cuts at: 1203 5224 6201 1203 Size: 4021 977 1327 Fragments arranged by size: 4021 1327 977 AgeI A'CCGG_T Cuts at: 2550 5156 2550 Size: 2606 3719 AhdI GACnn_n'nnGTC Cuts at: 3331 5054 3331 Size: 1723 4602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6325 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3467 1184 Size: 2283 4042 AlwNI CAG_nnn'CTG Cuts at: 4068 5640 4068 Size: 1572 4753 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 3634 3860 4012 6121 1493 Size: 2141 226 152 2109 1697 Fragments arranged by size: 2141 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 3650 1870 Size: 73 1707 4545 Fragments arranged by size: 4545 1707 73 BamHI G'GATC_C Cuts at: 2657 3655 4022 2657 Size: 998 367 4960 Fragments arranged by size: 4960 998 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3646 5101 618 Size: 325 217 627 1859 1455 1842 Fragments arranged by size: 1859 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2686 2785 2911 3021 3165 3623 3644 1391 Size: 1295 99 126 110 144 458 21 4072 Fragments arranged by size: 4072 1295 458 144 126 110 99 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2484 2820 3138 928 Size: 971 585 336 318 4115 Fragments arranged by size: 4115 971 585 336 318 BbvCI CC'TCA_GC Cuts at: 3423 3423 Size: 6325 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4516 4550 1903 Size: 34 2579 34 3678 Fragments arranged by size: 3678 2579 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4217 5427 4217 Size: 1210 5115 BclI T'GATC_A Cuts at: 1548 3409 1548 Size: 1861 4464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 136 Size: 107 122 71 631 605 3264 1525 Fragments arranged by size: 3264 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2671 6320 2671 Size: 3649 2676 BlpI GC'TnA_GC Cuts at: 3742 3742 Size: 6325 Bme1580I G_kGCm'C Cuts at: 1790 2398 4386 5542 1790 Size: 608 1988 1156 2573 Fragments arranged by size: 2573 1988 1156 608 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6325 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4056 5014 442 Size: 416 53 83 399 24 2639 958 1753 Fragments arranged by size: 2639 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2341 2663 3637 2341 Size: 322 974 5029 Fragments arranged by size: 5029 974 322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2208 2324 2504 2895 4985 1239 Size: 969 116 180 391 2090 2579 Fragments arranged by size: 2579 2090 969 391 180 116 Bpu10I CC'TnA_GC Cuts at: 3387 3423 3387 Size: 36 6289 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4450 5315 5613 4450 Size: 865 298 5162 Fragments arranged by size: 5162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2999 3334 3563 4988 882 Size: 617 1500 335 229 1425 2219 Fragments arranged by size: 2219 1500 1425 617 335 229 BsaAI yAC'GTr Cuts at: 493 1204 3040 3067 4116 493 Size: 711 1836 27 1049 2702 Fragments arranged by size: 2702 1836 1049 711 27 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4132 4514 5149 275 Size: 53 83 186 936 2599 382 635 1451 Fragments arranged by size: 2599 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2550 2660 4758 5156 5430 5577 5988 2550 Size: 110 2098 398 274 147 411 2887 Fragments arranged by size: 2887 2098 411 398 274 147 110 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2206 2236 3331 3361 3411 Size: 30 165 30 803 30 1095 30 50 Cuts at: 3411 3441 1178 Size: 30 4062 Fragments arranged by size: 4062 1095 803 165 50 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2211 2270 2321 2480 3079 3332 1186 Size: 1025 59 51 159 599 253 4179 Fragments arranged by size: 4179 1025 599 253 159 59 51 BseYI C'CCAG_C Cuts at: 2523 5528 6024 2523 Size: 3005 496 2824 Fragments arranged by size: 3005 2824 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3300 3387 1163 Size: 573 1564 87 4101 Fragments arranged by size: 4101 1564 573 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2520 3775 4536 5564 1540 Size: 275 186 519 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 519 275 186 BsiHKAI G_wGCw'C Cuts at: 2325 2686 2785 3021 3623 4386 4471 5542 2325 Size: 361 99 236 602 763 85 1071 3108 Fragments arranged by size: 3108 1071 763 602 361 236 99 85 BsmI GAATG_Cn' Cuts at: 3534 3836 3929 3534 Size: 302 93 5930 Fragments arranged by size: 5930 302 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2325 2398 2686 2785 2911 3021 3165 Size: 399 535 73 288 99 126 110 144 Cuts at: 3165 3623 3644 4386 4471 5542 1391 Size: 458 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 535 458 399 288 144 126 110 99 85 73 21 BspEI T'CCGG_A Cuts at: 5988 5988 Size: 6325 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2340 3193 3680 844 Size: 1496 853 487 3489 Fragments arranged by size: 3489 1496 853 487 BsrBI CCG'CTC Cuts at: 1394 1823 2275 2476 3034 3322 4212 1394 Size: 429 452 201 558 288 890 3507 Fragments arranged by size: 3507 890 558 452 429 288 201 BsrDI GCAATG_nn' Cuts at: 65 1248 4820 4994 65 Size: 1183 3572 174 1396 Fragments arranged by size: 3572 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2505 2550 4969 5156 1700 Size: 110 695 45 2419 187 2869 Fragments arranged by size: 2869 2419 695 187 110 45 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6325 BstXI CCAn_nnnn'nTGG Cuts at: 2636 3067 3478 2636 Size: 431 411 5483 Fragments arranged by size: 5483 431 411 BstYI r'GATC_y Cuts at: 1972 2657 2671 3655 4022 4407 4424 5876 5962 Size: 685 14 984 367 385 17 1452 86 Cuts at: 5962 5974 6320 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 984 685 385 367 346 86 17 14 12 BtgI C'CryG_G Cuts at: 513 1065 2635 3351 3525 513 Size: 552 1570 716 174 3313 Fragments arranged by size: 3313 1570 716 552 174 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 2447 3841 4635 4655 2447 Size: 1394 794 20 4117 Fragments arranged by size: 4117 1394 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6290 DraI TTT'AAA Cuts at: 3629 3629 Size: 6325 DraIII CAC_nnn'GTG Cuts at: 3365 3365 Size: 6325 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2283 3151 3772 Size: 54 1425 50 275 18 453 868 621 Cuts at: 3772 4661 6290 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 868 621 453 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3294 3324 3611 4255 1353 Size: 1941 30 287 644 3423 Fragments arranged by size: 3423 1941 644 287 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4912 5286 5432 387 Size: 1064 138 3323 374 146 1280 Fragments arranged by size: 3323 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2684 2783 3019 3621 2684 Size: 99 236 602 5388 Fragments arranged by size: 5388 602 236 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2208 2324 2504 2555 2895 3318 Size: 393 455 121 116 180 51 340 423 Cuts at: 3318 4388 4985 5772 6040 1239 Size: 1070 597 787 268 1524 Fragments arranged by size: 1524 1070 787 597 455 423 393 340 268 180 121 116 51 EcoO109I rG'GnC_Cy Cuts at: 2479 3293 3464 2479 Size: 814 171 5340 Fragments arranged by size: 5340 814 171 EcoRI G'AATT_C Cuts at: 3634 4012 3634 Size: 378 5947 EcoRV GAT'ATC Cuts at: 2134 2134 Size: 6325 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2066 2066 Size: 6325 FspI TGC'GCA Cuts at: 2453 4831 2453 Size: 2378 3947 HaeII r_GCGC'y Cuts at: 1678 5472 1678 Size: 3794 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2356 2388 3069 3101 3317 Size: 32 135 32 784 32 681 32 216 Cuts at: 3317 3349 3362 3394 4966 4998 5040 5072 1373 Size: 32 13 32 1572 32 42 32 2626 Fragments arranged by size: 2626 1572 784 681 216 135 42 32 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2030 3669 3915 669 Size: 988 281 92 1639 246 3079 Fragments arranged by size: 3079 1639 988 281 246 92 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3915 3915 Size: 6325 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3237 3924 3237 Size: 687 5638 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5439 5623 1305 Size: 4134 184 2007 Fragments arranged by size: 4134 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4503 Fragments arranged by size: 4503 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4283 4642 4801 518 Size: 648 3117 359 159 2042 Fragments arranged by size: 3117 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2302 2512 3557 3741 4418 5566 5811 Size: 501 195 210 1045 184 677 1148 245 Cuts at: 5811 1606 Size: 2120 Fragments arranged by size: 2120 1148 1045 677 501 245 210 195 184 NaeI GCC'GGC Cuts at: 2507 2507 Size: 6325 NcoI C'CATG_G Cuts at: 513 1065 2635 3351 513 Size: 552 1570 716 3487 Fragments arranged by size: 3487 1570 716 552 NdeI CA'TA_TG Cuts at: 387 3544 387 Size: 3157 3168 NgoMIV G'CCGG_C Cuts at: 2505 2505 Size: 6325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 3683 5228 1132 Size: 97 2454 1545 2229 Fragments arranged by size: 2454 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6325 PciI A'CATG_T Cuts at: 5224 5224 Size: 6325 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6325 PfoI T'CCnGG_A Cuts at: 2481 3335 2481 Size: 854 5471 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PmlI CAC'GTG Cuts at: 3040 3040 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2479 2479 Size: 6325 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 2430 2759 3895 2430 Size: 329 1136 4860 Fragments arranged by size: 4860 1136 329 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3366 3398 3366 Size: 32 6293 PstI C_TGCA'G Cuts at: 830 2202 2337 2358 2691 3216 3677 830 Size: 1372 135 21 333 525 461 3478 Fragments arranged by size: 3478 1372 525 461 333 135 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 PvuII CAG'CTG Cuts at: 2107 2302 2512 2107 Size: 195 210 5920 Fragments arranged by size: 5920 210 195 SacI G_AGCT'C Cuts at: 2686 2785 3021 3623 2686 Size: 99 236 602 5388 Fragments arranged by size: 5388 602 236 99 SalI G'TCGA_C Cuts at: 1936 3667 1936 Size: 1731 4594 SapI GCTCTTCn'nnn_ Cuts at: 3611 3611 Size: 6325 SbfI CC_TGCA'GG Cuts at: 2337 3677 2337 Size: 1340 4985 ScaI AGT'ACT Cuts at: 4573 6285 4573 Size: 1712 4613 SfcI C'TryA_G Cuts at: 826 1045 2198 2333 2354 2687 3212 3252 3673 Size: 219 1153 135 21 333 525 40 421 Cuts at: 3673 4808 5489 5680 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1153 1135 681 525 421 333 219 191 135 40 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 3652 1872 Size: 1780 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4429 5330 5592 820 Size: 197 926 2486 901 262 1553 Fragments arranged by size: 2486 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3683 1132 Size: 2551 3774 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6325 StyI C'CwwG_G Cuts at: 513 1065 2635 2741 3351 3471 3764 513 Size: 552 1570 106 610 120 293 3074 Fragments arranged by size: 3074 1570 610 552 293 120 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2642 3193 4352 4369 4522 4707 5152 Size: 1255 1062 551 1159 17 153 185 445 Cuts at: 5152 325 Size: 1498 Fragments arranged by size: 1498 1255 1159 1062 551 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4571 6283 96 Size: 274 80 33 51 1443 2594 1712 138 Fragments arranged by size: 2594 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2206 2238 3331 3363 3411 3443 6285 6317 2206 Size: 32 1093 32 48 32 2842 32 2214 Fragments arranged by size: 2842 2214 1093 48 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3589 1316 Size: 2273 4052 XbaI T'CTAG_A Cuts at: 3661 3661 Size: 6325 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2782 2782 Size: 6325 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6325 XmaI C'CCGG_G Cuts at: 1870 3650 1870 Size: 1780 4545 XmnI GAAnn'nnTTC Cuts at: 4454 4454 Size: 6325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI