(Linear) (Six-base) MAPSORT of: KIEE6778.seq Check: 1632 from: 1 to: 1620 KIEE6778.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 335 1188 1620 Size: 335 853 432 Fragments arranged by size: 853 432 335 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 550 1313 1620 Size: 82 468 763 307 Fragments arranged by size: 763 468 307 82 AgeI A'CCGG_T Cuts at: 0 545 1620 Size: 545 1075 AhdI GACnn_n'nnGTC Cuts at: 0 1326 1620 Size: 1326 294 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1462 1620 Size: 1462 158 BamHI G'GATC_C Cuts at: 0 652 1620 Size: 652 968 BanII G_rGCy'C Cuts at: 0 681 780 906 1016 1160 1618 1620 Size: 681 99 126 110 144 458 2 Fragments arranged by size: 681 458 144 126 110 99 2 BbsI GAAGACnn'nnnn_ Cuts at: 0 479 815 1133 1620 Size: 479 336 318 487 Fragments arranged by size: 487 479 336 318 BbvCI CC'TCA_GC Cuts at: 0 1418 1620 Size: 1418 202 BclI T'GATC_A Cuts at: 0 1404 1620 Size: 1404 216 BglII A'GATC_T Cuts at: 0 666 1620 Size: 666 954 Bme1580I G_kGCm'C Cuts at: 0 393 1620 Size: 393 1227 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 336 658 1620 Size: 336 322 962 Fragments arranged by size: 962 336 322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 203 319 499 890 1620 Size: 203 116 180 391 730 Fragments arranged by size: 730 391 203 180 116 Bpu10I CC'TnA_GC Cuts at: 0 1382 1418 1620 Size: 1382 36 202 Fragments arranged by size: 1382 202 36 BsaI GGTCTCn'nnnn_ Cuts at: 0 994 1329 1558 1620 Size: 994 335 229 62 Fragments arranged by size: 994 335 229 62 BsaAI yAC'GTr Cuts at: 0 1035 1062 1620 Size: 1035 27 558 Fragments arranged by size: 1035 558 27 BsaWI w'CCGG_w Cuts at: 0 545 655 1620 Size: 545 110 965 Fragments arranged by size: 965 545 110 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 201 231 1326 1356 1406 1436 1620 Size: 201 30 1095 30 50 30 184 Fragments arranged by size: 1095 201 184 50 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 206 265 316 475 1074 1327 1620 Size: 206 59 51 159 599 253 293 Fragments arranged by size: 599 293 253 206 159 59 51 BseYI C'CCAG_C Cuts at: 0 518 1620 Size: 518 1102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1295 1382 1620 Size: 1295 87 238 Fragments arranged by size: 1295 238 87 BsiEI CG_ry'CG Cuts at: 0 515 1620 Size: 515 1105 BsiHKAI G_wGCw'C Cuts at: 0 320 681 780 1016 1618 1620 Size: 320 361 99 236 602 2 Fragments arranged by size: 602 361 320 236 99 2 BsmI GAATG_Cn' Cuts at: 0 1529 1620 Size: 1529 91 Bsp1286I G_dGCh'C Cuts at: 0 320 393 681 780 906 1016 1160 1618 Size: 320 73 288 99 126 110 144 458 Cuts at: 1618 1620 Size: 2 Fragments arranged by size: 458 320 288 144 126 110 99 73 2 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 335 1188 1620 Size: 335 853 432 Fragments arranged by size: 853 432 335 BsrBI CCG'CTC Cuts at: 0 270 471 1029 1317 1620 Size: 270 201 558 288 303 Fragments arranged by size: 558 303 288 270 201 BsrFI r'CCGG_y Cuts at: 0 500 545 1620 Size: 500 45 1075 Fragments arranged by size: 1075 500 45 BstXI CCAn_nnnn'nTGG Cuts at: 0 631 1062 1473 1620 Size: 631 431 411 147 Fragments arranged by size: 631 431 411 147 BstYI r'GATC_y Cuts at: 0 652 666 1620 Size: 652 14 954 Fragments arranged by size: 954 652 14 BtgI C'CryG_G Cuts at: 0 630 1346 1520 1620 Size: 630 716 174 100 Fragments arranged by size: 716 630 174 100 BtsI GCAGTG_nn' Cuts at: 0 442 1620 Size: 442 1178 DraIII CAC_nnn'GTG Cuts at: 0 1360 1620 Size: 1360 260 EaeI y'GGCC_r Cuts at: 0 278 1146 1620 Size: 278 868 474 Fragments arranged by size: 868 474 278 EarI CTCTTCn'nnn_ Cuts at: 0 1289 1319 1606 1620 Size: 1289 30 287 14 Fragments arranged by size: 1289 287 30 14 EcoICRI GAG'CTC Cuts at: 0 679 778 1014 1616 1620 Size: 679 99 236 602 4 Fragments arranged by size: 679 602 236 99 4 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 203 319 499 550 890 1313 1620 Size: 82 121 116 180 51 340 423 307 Fragments arranged by size: 423 340 307 180 121 116 82 51 EcoO109I rG'GnC_Cy Cuts at: 0 474 1288 1459 1620 Size: 474 814 171 161 Fragments arranged by size: 814 474 171 161 EcoRV GAT'ATC Cuts at: 0 129 1620 Size: 129 1491 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 61 1620 Size: 61 1559 FspI TGC'GCA Cuts at: 0 448 1620 Size: 448 1172 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 351 383 1064 1096 1312 1344 1357 1389 Size: 351 32 681 32 216 32 13 32 Cuts at: 1389 1620 Size: 231 Fragments arranged by size: 681 351 231 216 32 32 32 32 13 HincII GTy'rAC Cuts at: 0 25 1620 Size: 25 1595 MfeI C'AATT_G Cuts at: 0 1232 1620 Size: 1232 388 MspA1I CmG'CkG Cuts at: 0 102 297 507 1552 1620 Size: 102 195 210 1045 68 Fragments arranged by size: 1045 210 195 102 68 NaeI GCC'GGC Cuts at: 0 502 1620 Size: 502 1118 NcoI C'CATG_G Cuts at: 0 630 1346 1620 Size: 630 716 274 Fragments arranged by size: 716 630 274 NdeI CA'TA_TG Cuts at: 0 1539 1620 Size: 1539 81 NgoMIV G'CCGG_C Cuts at: 0 500 1620 Size: 500 1120 PfoI T'CCnGG_A Cuts at: 0 476 1330 1620 Size: 476 854 290 Fragments arranged by size: 854 476 290 PmlI CAC'GTG Cuts at: 0 1035 1620 Size: 1035 585 PpuMI rG'GwC_Cy Cuts at: 0 474 1620 Size: 474 1146 PsiI TTA'TAA Cuts at: 0 425 754 1620 Size: 425 329 866 Fragments arranged by size: 866 425 329 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1361 1393 1620 Size: 1361 32 227 Fragments arranged by size: 1361 227 32 PstI C_TGCA'G Cuts at: 0 197 332 353 686 1211 1620 Size: 197 135 21 333 525 409 Fragments arranged by size: 525 409 333 197 135 21 PvuII CAG'CTG Cuts at: 0 102 297 507 1620 Size: 102 195 210 1113 Fragments arranged by size: 1113 210 195 102 SacI G_AGCT'C Cuts at: 0 681 780 1016 1618 1620 Size: 681 99 236 602 2 Fragments arranged by size: 681 602 236 99 2 SapI GCTCTTCn'nnn_ Cuts at: 0 1606 1620 Size: 1606 14 SbfI CC_TGCA'GG Cuts at: 0 332 1620 Size: 332 1288 SfcI C'TryA_G Cuts at: 0 193 328 349 682 1207 1247 1620 Size: 193 135 21 333 525 40 373 Fragments arranged by size: 525 373 333 193 135 40 21 StyI C'CwwG_G Cuts at: 0 630 736 1346 1466 1620 Size: 630 106 610 120 154 Fragments arranged by size: 630 610 154 120 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 637 1188 1620 Size: 637 551 432 Fragments arranged by size: 637 551 432 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 201 233 1326 1358 1406 1438 1620 Size: 201 32 1093 32 48 32 182 Fragments arranged by size: 1093 201 182 48 32 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1584 1620 Size: 1584 36 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 777 1620 Size: 777 843 Enzymes that do cut: AarI AcuI AgeI AhdI AlfI BamHI BanII BbsI BbvCI BclI BglII Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BstXI BstYI BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI FspI Hin4I HincII MfeI MspA1I NaeI NcoI NdeI NgoMIV PfoI PmlI PpuMI PsiI PsrI PstI PvuII SacI SapI SbfI SfcI StyI TaqII TstI Tth111I XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BcgI BciVI BfrBI BglI BlpI BmgBI BmrI BmtI BpuEI BsaBI BsaHI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoNI EcoRI FseI FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI MscI MslI NarI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PspOMI PspXI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI XbaI XhoI XmaI XmnI ZraI