(Circular) (Six-base) MAPSORT of: pFN21AE5776.seq Check: 9840 from: 1 to: 6577 pFN21AE5776 6577 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4387 278 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3920 1937 Size: 1983 4594 Acc65I G'GTAC_C Cuts at: 3898 3898 Size: 6577 AclI AA'CG_TT Cuts at: 1563 4704 5077 1563 Size: 3141 373 3063 Fragments arranged by size: 3141 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2334 3831 4640 6024 6292 1632 Size: 702 1497 809 1384 268 1917 Fragments arranged by size: 1917 1497 1384 809 702 268 AfeI AGC'GCT Cuts at: 3056 3056 Size: 6577 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6380 AflIII A'CryG_T Cuts at: 1203 5476 6453 1203 Size: 4273 977 1327 Fragments arranged by size: 4273 1327 977 AgeI A'CCGG_T Cuts at: 5408 5408 Size: 6577 AhdI GACnn_n'nnGTC Cuts at: 5306 5306 Size: 6577 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6577 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3290 3438 1184 Size: 2106 148 4323 Fragments arranged by size: 4323 2106 148 AlwNI CAG_nnn'CTG Cuts at: 2372 4320 5892 2372 Size: 1948 1572 3057 Fragments arranged by size: 3057 1948 1572 ApaLI G'TGCA_C Cuts at: 3275 4634 5790 3275 Size: 1359 1156 4062 Fragments arranged by size: 4062 1359 1156 ApoI r'AATT_y Cuts at: 1493 2436 3221 3755 3886 4112 4264 6373 1493 Size: 943 785 534 131 226 152 2109 1697 Fragments arranged by size: 2109 1697 943 785 534 226 152 131 AseI AT'TA_AT Cuts at: 160 1032 2781 5131 160 Size: 872 1749 2350 1606 Fragments arranged by size: 2350 1749 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 AvaI C'yCGr_G Cuts at: 1870 1943 2015 2535 3789 3902 1870 Size: 73 72 520 1254 113 4545 Fragments arranged by size: 4545 1254 520 113 73 72 BamHI G'GATC_C Cuts at: 3907 4274 3907 Size: 367 6210 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3339 3629 3898 5353 618 Size: 325 217 627 1552 290 269 1455 1842 Fragments arranged by size: 1842 1552 1455 627 325 290 269 217 BanII G_rGCy'C Cuts at: 1391 3896 1391 Size: 2505 4072 BbeI G_GCGC'C Cuts at: 3343 3343 Size: 6577 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2322 2882 3753 928 Size: 971 423 560 871 3752 Fragments arranged by size: 3752 971 871 560 423 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4768 4802 1903 Size: 34 2831 34 3678 Fragments arranged by size: 3678 2831 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3298 4469 5679 3298 Size: 1171 1210 4196 Fragments arranged by size: 4196 1210 1171 BclI T'GATC_A Cuts at: 1548 3184 1548 Size: 1636 4941 BfrBI ATG'CAT Cuts at: 2693 2693 Size: 6577 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5188 136 Size: 107 122 71 631 605 3516 1525 Fragments arranged by size: 3516 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3142 6572 3142 Size: 3430 3147 BlpI GC'TnA_GC Cuts at: 2042 3994 2042 Size: 1952 4625 Bme1580I G_kGCm'C Cuts at: 1790 3279 4638 5794 1790 Size: 1489 1359 1156 2573 Fragments arranged by size: 2573 1489 1359 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6577 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2071 2571 3266 Size: 416 53 83 399 24 654 500 695 Cuts at: 3266 4308 5266 442 Size: 1042 958 1753 Fragments arranged by size: 1753 1042 958 695 654 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6577 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2208 5237 1239 Size: 969 3029 2579 Fragments arranged by size: 3029 2579 969 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4702 5567 5865 4702 Size: 865 298 5414 Fragments arranged by size: 5414 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2291 3564 5240 882 Size: 617 792 1273 1676 2219 Fragments arranged by size: 2219 1676 1273 792 617 BsaAI yAC'GTr Cuts at: 493 1204 4368 493 Size: 711 3164 2702 Fragments arranged by size: 3164 2702 711 BsaBI GATnn'nnATC Cuts at: 3189 6571 3189 Size: 3382 3195 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3340 4384 4766 5401 Size: 53 83 186 936 1807 1044 382 635 Cuts at: 5401 275 Size: 1451 Fragments arranged by size: 1807 1451 1044 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5010 5408 5682 5829 6240 5010 Size: 398 274 147 411 5347 Fragments arranged by size: 5347 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6352 Fragments arranged by size: 6352 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2237 2475 3065 3153 3341 3344 1186 Size: 1051 238 590 88 188 3 4419 Fragments arranged by size: 4419 1051 590 238 188 88 3 BseYI C'CCAG_C Cuts at: 5780 6276 5780 Size: 496 6081 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2825 1163 Size: 573 1089 4915 Fragments arranged by size: 4915 1089 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4027 4788 5816 1540 Size: 275 186 2026 761 1028 2301 Fragments arranged by size: 2301 2026 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3042 3279 3532 4638 4723 5794 3042 Size: 237 253 1106 85 1071 3825 Fragments arranged by size: 3825 1106 1071 253 237 85 BsmI GAATG_Cn' Cuts at: 2073 3241 3780 4088 4181 2073 Size: 1168 539 308 93 4469 Fragments arranged by size: 4469 1168 539 308 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3449 3449 Size: 6577 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3042 3279 3532 3896 4638 4723 5794 Size: 399 1252 237 253 364 742 85 1071 Cuts at: 5794 1391 Size: 2174 Fragments arranged by size: 2174 1252 1071 742 399 364 253 237 85 BspEI T'CCGG_A Cuts at: 6240 6240 Size: 6577 BspHI T'CATG_A Cuts at: 2440 2944 4466 6196 2440 Size: 504 1522 1730 2821 Fragments arranged by size: 2821 1730 1522 504 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2797 3932 844 Size: 1953 1135 3489 Fragments arranged by size: 3489 1953 1135 BsrBI CCG'CTC Cuts at: 1394 1823 2192 4464 1394 Size: 429 369 2272 3507 Fragments arranged by size: 3507 2272 429 369 BsrDI GCAATG_nn' Cuts at: 65 1248 3520 5072 5246 65 Size: 1183 2272 1552 174 1396 Fragments arranged by size: 2272 1552 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3336 5221 5408 1700 Size: 110 1526 1885 187 2869 Fragments arranged by size: 2869 1885 1526 187 110 BsrGI T'GTAC_A Cuts at: 96 2972 96 Size: 2876 3701 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6577 BssSI C'ACGA_G Cuts at: 4637 5649 4637 Size: 1012 5565 BstAPI GCAn_nnn'nTGC Cuts at: 794 2351 2803 794 Size: 1557 452 4568 Fragments arranged by size: 4568 1557 452 BstBI TT'CG_AA Cuts at: 5381 5381 Size: 6577 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6577 BstYI r'GATC_y Cuts at: 1972 2010 3142 3607 3907 4274 4659 4676 6128 Size: 38 1132 465 300 367 385 17 1452 Cuts at: 6128 6214 6226 6572 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1132 465 385 367 346 300 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5086 Fragments arranged by size: 5086 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2326 487 Size: 683 56 1100 4738 Fragments arranged by size: 4738 1100 683 56 BtsI GCAGTG_nn' Cuts at: 2804 2876 3432 4093 4887 4907 2804 Size: 72 556 661 794 20 4474 Fragments arranged by size: 4474 794 661 556 72 20 ClaI AT'CG_AT Cuts at: 1554 2568 1554 Size: 1014 5563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6542 DraI TTT'AAA Cuts at: 3226 3881 3226 Size: 655 5922 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5584 809 Size: 507 4268 1802 Fragments arranged by size: 4268 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3009 3468 4024 Size: 54 1425 50 275 18 1179 459 556 Cuts at: 4024 4913 6542 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1179 889 556 459 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4024 1537 Size: 275 2212 4090 Fragments arranged by size: 4090 2212 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2319 4507 1353 Size: 966 2188 3423 Fragments arranged by size: 3423 2188 966 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5164 5538 5684 387 Size: 1064 138 3575 374 146 1280 Fragments arranged by size: 3575 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2208 2334 3831 4640 5237 6024 6292 Size: 393 576 126 1497 809 597 787 268 Cuts at: 6292 1239 Size: 1524 Fragments arranged by size: 1524 1497 809 787 597 576 393 268 126 EcoO109I rG'GnC_Cy Cuts at: 2593 2593 Size: 6577 EcoRI G'AATT_C Cuts at: 3886 4264 3886 Size: 378 6199 FspI TGC'GCA Cuts at: 3779 5083 3779 Size: 1304 5273 HaeII r_GCGC'y Cuts at: 1678 3058 3343 5724 1678 Size: 1380 285 2381 2531 Fragments arranged by size: 2531 2381 1380 285 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2926 2958 5218 5250 5292 Size: 32 135 32 1354 32 2260 32 42 Cuts at: 5292 5324 1373 Size: 32 2626 Fragments arranged by size: 2626 2260 1354 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3921 4167 669 Size: 988 281 1983 246 3079 Fragments arranged by size: 3079 1983 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6577 HpaI GTT'AAC Cuts at: 4167 4167 Size: 6577 KasI G'GCGC_C Cuts at: 3339 3339 Size: 6577 KpnI G_GTAC'C Cuts at: 3902 3902 Size: 6577 MfeI C'AATT_G Cuts at: 4176 4176 Size: 6577 MluI A'CGCG_T Cuts at: 6453 6453 Size: 6577 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3411 3544 5691 5875 1305 Size: 2106 133 2147 184 2007 Fragments arranged by size: 2147 2106 2007 184 133 MscI TGG'CCA Cuts at: 10 64 1489 1832 3011 3470 10 Size: 54 1425 343 1179 459 3117 Fragments arranged by size: 3117 1425 1179 459 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2445 2496 4535 4894 5053 518 Size: 648 1279 51 2039 359 159 2042 Fragments arranged by size: 2042 2039 1279 648 359 159 51 MspA1I CmG'CkG Cuts at: 1606 3993 4670 5818 6063 1606 Size: 2387 677 1148 245 2120 Fragments arranged by size: 2387 2120 1148 677 245 NarI GG'CG_CC Cuts at: 3340 3340 Size: 6577 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5086 Fragments arranged by size: 5086 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6577 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6577 NotI GC'GGCC_GC Cuts at: 4024 4024 Size: 6577 NsiI A_TGCA'T Cuts at: 2695 2695 Size: 6577 NspI r_CATG'y Cuts at: 1132 1229 2495 3084 3290 3422 3586 3935 5480 Size: 97 1266 589 206 132 164 349 1545 Cuts at: 5480 1132 Size: 2229 Fragments arranged by size: 2229 1545 1266 589 349 206 164 132 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6577 PciI A'CATG_T Cuts at: 5476 5476 Size: 6577 PflMI CCAn_nnn'nTGG Cuts at: 1108 2840 3069 3364 3626 3834 1108 Size: 1732 229 295 262 208 3851 Fragments arranged by size: 3851 1732 295 262 229 208 PmeI GTTT'AAAC Cuts at: 3881 3881 Size: 6577 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4646 4678 6159 6191 4646 Size: 32 1481 32 5032 Fragments arranged by size: 5032 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6577 PsiI TTA'TAA Cuts at: 4147 4147 Size: 6577 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2913 2945 2913 Size: 32 6545 PstI C_TGCA'G Cuts at: 830 3427 3929 830 Size: 2597 502 3478 Fragments arranged by size: 3478 2597 502 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6577 SalI G'TCGA_C Cuts at: 1936 3919 1936 Size: 1983 4594 SbfI CC_TGCA'GG Cuts at: 3929 3929 Size: 6577 ScaI AGT'ACT Cuts at: 2864 4825 6537 2864 Size: 1961 1712 2904 Fragments arranged by size: 2904 1961 1712 SexAI A'CCwGG_T Cuts at: 2229 2229 Size: 6577 SfcI C'TryA_G Cuts at: 826 1045 2663 2925 3423 3925 5060 5741 5932 Size: 219 1618 262 498 502 1135 681 191 Cuts at: 5932 826 Size: 1471 Fragments arranged by size: 1618 1471 1135 681 502 498 262 219 191 SfoI GGC'GCC Cuts at: 3341 3341 Size: 6577 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6577 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6577 SmaI CCC'GGG Cuts at: 1872 2017 2537 3904 1872 Size: 145 520 1367 4545 Fragments arranged by size: 4545 1367 520 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 3789 4681 5582 5844 820 Size: 197 926 1846 892 901 262 1553 Fragments arranged by size: 1846 1553 926 901 892 262 197 SnaBI TAC'GTA Cuts at: 493 4368 493 Size: 3875 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6577 SphI G_CATG'C Cuts at: 1132 3422 3586 3935 1132 Size: 2290 164 349 3774 Fragments arranged by size: 3774 2290 349 164 SrfI GCCC'GGGC Cuts at: 2537 2537 Size: 6577 SspI AAT'ATT Cuts at: 5 52 2275 4501 5 Size: 47 2223 2226 2081 Fragments arranged by size: 2226 2223 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6577 StyI C'CwwG_G Cuts at: 513 1065 2004 2217 3828 3864 4016 513 Size: 552 939 213 1611 36 152 3074 Fragments arranged by size: 3074 1611 939 552 213 152 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2791 3356 4604 4621 4774 4959 5404 Size: 1255 1211 565 1248 17 153 185 445 Cuts at: 5404 325 Size: 1498 Fragments arranged by size: 1498 1255 1248 1211 565 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2862 2972 4823 Size: 274 80 33 51 1443 885 110 1851 Cuts at: 4823 6535 96 Size: 1712 138 Fragments arranged by size: 1851 1712 1443 885 274 138 110 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2131 2163 2783 2815 6537 6569 2131 Size: 32 620 32 3722 32 2139 Fragments arranged by size: 3722 2139 620 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2639 1316 Size: 1323 5254 XbaI T'CTAG_A Cuts at: 3322 3913 3322 Size: 591 5986 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3365 3365 Size: 6577 XhoI C'TCGA_G Cuts at: 1943 3789 1943 Size: 1846 4731 XmaI C'CCGG_G Cuts at: 1870 2015 2535 3902 1870 Size: 145 520 1367 4545 Fragments arranged by size: 4545 1367 520 145 XmnI GAAnn'nnTTC Cuts at: 3441 3675 4706 3441 Size: 234 1031 5312 Fragments arranged by size: 5312 1031 234 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4385 276 Size: 53 83 186 936 2851 2468 Fragments arranged by size: 2851 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SfiI SwaI