(Circular) (Six-base) MAPSORT of: pFN21AE5681.seq Check: 8634 from: 1 to: 6271 pFN21AE5681 6271 bp With 182 enzymes: SgfI * December 8, 2014 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4081 278 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2700 3614 1937 Size: 763 914 4594 Fragments arranged by size: 4594 914 763 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 3273 4398 4771 1563 Size: 1710 1125 373 3063 Fragments arranged by size: 3063 1710 1125 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 2480 2618 3223 4334 5718 5986 1632 Size: 776 72 138 605 1111 1384 268 1917 Fragments arranged by size: 1917 1384 1111 776 605 268 138 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 3465 5170 6147 1203 Size: 2262 1705 977 1327 Fragments arranged by size: 2262 1705 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6271 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3012 1184 Size: 1828 4443 AlwNI CAG_nnn'CTG Cuts at: 2374 2553 4014 5586 2374 Size: 179 1461 1572 3059 Fragments arranged by size: 3059 1572 1461 179 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 2176 2582 3580 3806 3958 6067 1493 Size: 683 406 998 226 152 2109 1697 Fragments arranged by size: 2109 1697 998 683 406 226 152 AseI AT'TA_AT Cuts at: 160 1032 2592 4825 160 Size: 872 1560 2233 1606 Fragments arranged by size: 2233 1606 1560 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2141 3188 3596 1870 Size: 73 134 64 1047 408 4545 Fragments arranged by size: 4545 1047 408 134 73 64 BamHI G'GATC_C Cuts at: 2486 3601 3968 2486 Size: 1115 367 4789 Fragments arranged by size: 4789 1115 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2081 2670 2744 3493 3592 Size: 325 217 627 294 589 74 749 99 Cuts at: 3592 5047 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 749 627 589 325 294 217 99 74 BanII G_rGCy'C Cuts at: 1391 2160 2817 3590 1391 Size: 769 657 773 4072 Fragments arranged by size: 4072 773 769 657 BbeI G_GCGC'C Cuts at: 2085 2085 Size: 6271 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2606 2845 3309 928 Size: 971 707 239 464 3890 Fragments arranged by size: 3890 971 707 464 239 BbvCI CC'TCA_GC Cuts at: 3365 3398 3365 Size: 33 6238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2398 2432 4462 4496 1903 Size: 34 461 34 2030 34 3678 Fragments arranged by size: 3678 2030 461 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4163 5373 4163 Size: 1210 5061 BclI T'GATC_A Cuts at: 1548 1548 Size: 6271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4882 136 Size: 107 122 71 631 605 337 2873 1525 Fragments arranged by size: 2873 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2625 6266 2625 Size: 3641 2630 BlpI GC'TnA_GC Cuts at: 2054 3688 2054 Size: 1634 4637 Bme1580I G_kGCm'C Cuts at: 1790 2747 2991 3496 4332 5488 1790 Size: 957 244 505 836 1156 2573 Fragments arranged by size: 2573 1156 957 836 505 244 BmgBI CAC'GTC Cuts at: 1318 2833 2862 2992 1318 Size: 1515 29 130 4597 Fragments arranged by size: 4597 1515 130 29 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2440 2563 2701 Size: 416 53 83 399 24 1023 123 138 Cuts at: 2701 3484 3499 4002 4960 442 Size: 783 15 503 958 1753 Fragments arranged by size: 1753 1023 958 783 503 416 399 138 123 83 53 24 15 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2146 2831 3519 4931 1239 Size: 907 685 688 1412 2579 Fragments arranged by size: 2579 1412 907 688 685 Bpu10I CC'TnA_GC Cuts at: 3365 3398 3365 Size: 33 6238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4396 5261 5559 4396 Size: 865 298 5108 Fragments arranged by size: 5108 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2162 4934 882 Size: 617 663 2772 2219 Fragments arranged by size: 2772 2219 663 617 BsaAI yAC'GTr Cuts at: 493 1204 4062 493 Size: 711 2858 2702 Fragments arranged by size: 2858 2702 711 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2082 2703 2772 3071 Size: 53 83 186 936 549 621 69 299 Cuts at: 3071 4078 4460 5095 275 Size: 1007 382 635 1451 Fragments arranged by size: 1451 1007 936 635 621 549 382 299 186 83 69 53 BsaWI w'CCGG_w Cuts at: 2184 4704 5102 5376 5523 5934 2184 Size: 2520 398 274 147 411 2521 Fragments arranged by size: 2521 2520 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2760 2790 2799 2829 1178 Size: 30 165 30 1357 30 9 30 4620 Fragments arranged by size: 4620 1357 165 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2807 3098 1186 Size: 1621 291 4359 Fragments arranged by size: 4359 1621 291 BseYI C'CCAG_C Cuts at: 3019 3077 3456 5474 5970 3019 Size: 58 379 2018 496 3320 Fragments arranged by size: 3320 2018 496 379 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2813 1163 Size: 573 1077 4621 Fragments arranged by size: 4621 1077 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3417 3721 4482 5510 1540 Size: 275 186 1416 304 761 1028 2301 Fragments arranged by size: 2301 1416 1028 761 304 275 186 BsiHKAI G_wGCw'C Cuts at: 2055 2243 2817 4332 4417 5488 2055 Size: 188 574 1515 85 1071 2838 Fragments arranged by size: 2838 1515 1071 574 188 85 BsmI GAATG_Cn' Cuts at: 3782 3875 3782 Size: 93 6178 BsmBI CGTCTCn'nnnn_ Cuts at: 2763 2763 Size: 6271 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2160 2243 2747 2817 2991 3496 Size: 399 265 105 83 504 70 174 505 Cuts at: 3496 3590 4332 4417 5488 1391 Size: 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 505 504 399 265 174 105 94 85 83 70 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 3483 4160 5890 3483 Size: 677 1730 3864 Fragments arranged by size: 3864 1730 677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2640 2835 3626 844 Size: 1796 195 791 3489 Fragments arranged by size: 3489 1796 791 195 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2293 4766 4940 65 Size: 1183 1045 2473 174 1396 Fragments arranged by size: 2473 1396 1183 1045 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2396 3129 4915 5102 1700 Size: 110 586 733 1786 187 2869 Fragments arranged by size: 2869 1786 733 586 187 110 BsrGI T'GTAC_A Cuts at: 96 2544 96 Size: 2448 3823 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 2044 2374 794 Size: 1250 330 4691 Fragments arranged by size: 4691 1250 330 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 2135 1184 Size: 951 5320 BstXI CCAn_nnnn'nTGG Cuts at: 3052 3052 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2486 2625 3601 3968 4353 4370 5822 5908 Size: 514 139 976 367 385 17 1452 86 Cuts at: 5908 5920 6266 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 976 514 385 367 346 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2061 3160 513 Size: 552 939 57 1099 3624 Fragments arranged by size: 3624 1099 939 552 57 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 2258 2994 3787 4581 4601 2258 Size: 736 793 794 20 3928 Fragments arranged by size: 3928 794 793 736 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2831 5278 809 Size: 507 1515 2447 1802 Fragments arranged by size: 2447 1802 1515 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2981 3180 3718 Size: 54 1425 50 275 18 1151 199 538 Cuts at: 3718 4607 6236 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1151 889 538 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3718 1537 Size: 275 1906 4090 Fragments arranged by size: 4090 1906 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3044 4201 1353 Size: 1691 1157 3423 Fragments arranged by size: 3423 1691 1157 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2878 4858 5232 5378 387 Size: 1064 138 1289 1980 374 146 1280 Fragments arranged by size: 1980 1289 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2815 2815 Size: 6271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2408 2480 2618 2831 3223 3519 Size: 393 514 262 72 138 213 392 296 Cuts at: 3519 4334 4931 5718 5986 1239 Size: 815 597 787 268 1524 Fragments arranged by size: 1524 815 787 597 514 393 392 296 268 262 213 138 72 EcoNI CCTnn'n_nnAGG Cuts at: 3122 3122 Size: 6271 EcoO109I rG'GnC_Cy Cuts at: 2073 2442 3343 3394 2073 Size: 369 901 51 4950 Fragments arranged by size: 4950 901 369 51 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2222 3223 2222 Size: 1001 5270 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 2061 2085 2877 3381 5418 1678 Size: 383 24 792 504 2037 2531 Fragments arranged by size: 2531 2037 792 504 383 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2760 2792 4912 4944 4986 Size: 32 135 32 1188 32 2120 32 42 Cuts at: 4986 5018 1373 Size: 32 2626 Fragments arranged by size: 2626 2120 1188 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2701 3615 3861 669 Size: 988 281 763 914 246 3079 Fragments arranged by size: 3079 988 914 763 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6271 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KasI G'GCGC_C Cuts at: 2081 2081 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2844 5385 5569 1305 Size: 1539 2541 184 2007 Fragments arranged by size: 2541 2007 1539 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2983 10 Size: 54 1425 343 1151 3298 Fragments arranged by size: 3298 1425 1151 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 3173 4229 4588 4747 518 Size: 648 1374 633 1056 359 159 2042 Fragments arranged by size: 2042 1374 1056 648 633 359 159 MspA1I CmG'CkG Cuts at: 1606 2041 2063 3019 3687 4364 5512 5757 1606 Size: 435 22 956 668 677 1148 245 2120 Fragments arranged by size: 2120 1148 956 677 668 435 245 22 NaeI GCC'GGC Cuts at: 2398 3131 2398 Size: 733 5538 NarI GG'CG_CC Cuts at: 2082 2082 Size: 6271 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4780 Fragments arranged by size: 4780 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6271 NgoMIV G'CCGG_C Cuts at: 2396 3129 2396 Size: 733 5538 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 3629 5174 1132 Size: 97 2400 1545 2229 Fragments arranged by size: 2400 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3263 3347 1348 Size: 1915 84 4272 Fragments arranged by size: 4272 1915 84 PciI A'CATG_T Cuts at: 5170 5170 Size: 6271 PflMI CCAn_nnn'nTGG Cuts at: 1108 3019 3228 1108 Size: 1911 209 4151 Fragments arranged by size: 4151 1911 209 PfoI T'CCnGG_A Cuts at: 2778 2778 Size: 6271 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3343 3343 Size: 6271 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PstI C_TGCA'G Cuts at: 830 2197 2637 3001 3126 3623 830 Size: 1367 440 364 125 497 3478 Fragments arranged by size: 3478 1367 497 440 364 125 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 2041 3019 2041 Size: 978 5293 SacI G_AGCT'C Cuts at: 2817 2817 Size: 6271 SacII CC_GC'GG Cuts at: 2064 2064 Size: 6271 SalI G'TCGA_C Cuts at: 1936 2699 3613 1936 Size: 763 914 4594 Fragments arranged by size: 4594 914 763 SanDI GG'GwC_CC Cuts at: 3343 3343 Size: 6271 SbfI CC_TGCA'GG Cuts at: 2637 3126 3623 2637 Size: 489 497 5285 Fragments arranged by size: 5285 497 489 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SfcI C'TryA_G Cuts at: 826 1045 2193 2633 2997 3122 3619 4754 5435 Size: 219 1148 440 364 125 497 1135 681 Cuts at: 5435 5626 826 Size: 191 1471 Fragments arranged by size: 1471 1148 1135 681 497 440 364 219 191 125 SfoI GGC'GCC Cuts at: 2083 2083 Size: 6271 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4375 5276 5538 820 Size: 197 926 2432 901 262 1553 Fragments arranged by size: 2432 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3629 1132 Size: 2497 3774 SspI AAT'ATT Cuts at: 5 52 4195 5 Size: 47 4143 2081 Fragments arranged by size: 4143 2081 47 StuI AGG'CCT Cuts at: 1517 3145 3437 1517 Size: 1628 292 4351 Fragments arranged by size: 4351 1628 292 StyI C'CwwG_G Cuts at: 513 1065 2004 3438 3710 513 Size: 552 939 1434 272 3074 Fragments arranged by size: 3074 1434 939 552 272 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2636 3403 4298 4315 4468 4653 5098 Size: 1255 1056 767 895 17 153 185 445 Cuts at: 5098 325 Size: 1498 Fragments arranged by size: 1498 1255 1056 895 767 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 4517 6229 Size: 274 80 33 51 1443 567 1973 1712 Cuts at: 6229 96 Size: 138 Fragments arranged by size: 1973 1712 1443 567 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6231 6263 6231 Size: 32 6239 Tth111I GACn'n_nGTC Cuts at: 1316 2774 1316 Size: 1458 4813 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2737 2737 Size: 6271 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6271 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 2583 3543 4400 2583 Size: 960 857 4454 Fragments arranged by size: 4454 960 857 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4079 276 Size: 53 83 186 936 2545 2468 Fragments arranged by size: 2545 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI