(Linear) (Six-base) MAPSORT of: KIEE5681.seq Check: 7335 from: 1 to: 1566 KIEE5681.orf With 182 enzymes: SgfI * December 5, 2014 13:06 .. AccI GT'mk_AC Cuts at: 0 695 1566 Size: 695 871 AclI AA'CG_TT Cuts at: 0 1268 1566 Size: 1268 298 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 403 475 613 1218 1566 Size: 403 72 138 605 348 Fragments arranged by size: 605 403 348 138 72 AflIII A'CryG_T Cuts at: 0 1460 1566 Size: 1460 106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1007 1566 Size: 1007 559 AlwNI CAG_nnn'CTG Cuts at: 0 369 548 1566 Size: 369 179 1018 Fragments arranged by size: 1018 369 179 ApoI r'AATT_y Cuts at: 0 171 577 1566 Size: 171 406 989 Fragments arranged by size: 989 406 171 AseI AT'TA_AT Cuts at: 0 587 1566 Size: 587 979 AvaI C'yCGr_G Cuts at: 0 72 136 1183 1566 Size: 72 64 1047 383 Fragments arranged by size: 1047 383 72 64 BamHI G'GATC_C Cuts at: 0 481 1566 Size: 481 1085 BanI G'GyrC_C Cuts at: 0 76 665 739 1488 1566 Size: 76 589 74 749 78 Fragments arranged by size: 749 589 78 76 74 BanII G_rGCy'C Cuts at: 0 155 812 1566 Size: 155 657 754 Fragments arranged by size: 754 657 155 BbeI G_GCGC'C Cuts at: 0 80 1566 Size: 80 1486 BbsI GAAGACnn'nnnn_ Cuts at: 0 601 840 1304 1566 Size: 601 239 464 262 Fragments arranged by size: 601 464 262 239 BbvCI CC'TCA_GC Cuts at: 0 1360 1393 1566 Size: 1360 33 173 Fragments arranged by size: 1360 173 33 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 393 427 1566 Size: 393 34 1139 Fragments arranged by size: 1139 393 34 BglII A'GATC_T Cuts at: 0 620 1566 Size: 620 946 BlpI GC'TnA_GC Cuts at: 0 49 1566 Size: 49 1517 Bme1580I G_kGCm'C Cuts at: 0 742 986 1491 1566 Size: 742 244 505 75 Fragments arranged by size: 742 505 244 75 BmgBI CAC'GTC Cuts at: 0 828 857 987 1566 Size: 828 29 130 579 Fragments arranged by size: 828 579 130 29 BmrI ACTGGGnnnn_n' Cuts at: 0 435 558 696 1479 1494 1566 Size: 435 123 138 783 15 72 Fragments arranged by size: 783 435 138 123 72 15 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 826 1514 1566 Size: 141 685 688 52 Fragments arranged by size: 688 685 141 52 Bpu10I CC'TnA_GC Cuts at: 0 1360 1393 1566 Size: 1360 33 173 Fragments arranged by size: 1360 173 33 BsaI GGTCTCn'nnnn_ Cuts at: 0 157 1566 Size: 157 1409 BsaHI Gr'CG_yC Cuts at: 0 77 698 767 1066 1566 Size: 77 621 69 299 500 Fragments arranged by size: 621 500 299 77 69 BsaWI w'CCGG_w Cuts at: 0 179 1566 Size: 179 1387 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 755 785 794 824 1566 Size: 755 30 9 30 742 Fragments arranged by size: 755 742 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 802 1093 1566 Size: 802 291 473 Fragments arranged by size: 802 473 291 BseYI C'CCAG_C Cuts at: 0 1014 1072 1451 1566 Size: 1014 58 379 115 Fragments arranged by size: 1014 379 115 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 808 1566 Size: 808 758 BsiEI CG_ry'CG Cuts at: 0 1412 1566 Size: 1412 154 BsiHKAI G_wGCw'C Cuts at: 0 50 238 812 1566 Size: 50 188 574 754 Fragments arranged by size: 754 574 188 50 BsmBI CGTCTCn'nnnn_ Cuts at: 0 758 1566 Size: 758 808 Bsp1286I G_dGCh'C Cuts at: 0 50 155 238 742 812 986 1491 1566 Size: 50 105 83 504 70 174 505 75 Fragments arranged by size: 505 504 174 105 83 75 70 50 BspHI T'CATG_A Cuts at: 0 1478 1566 Size: 1478 88 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 635 830 1566 Size: 635 195 736 Fragments arranged by size: 736 635 195 BsrDI GCAATG_nn' Cuts at: 0 288 1566 Size: 288 1278 BsrFI r'CCGG_y Cuts at: 0 391 1124 1566 Size: 391 733 442 Fragments arranged by size: 733 442 391 BsrGI T'GTAC_A Cuts at: 0 539 1566 Size: 539 1027 BstAPI GCAn_nnn'nTGC Cuts at: 0 39 369 1566 Size: 39 330 1197 Fragments arranged by size: 1197 330 39 BstEII G'GTnAC_C Cuts at: 0 130 1566 Size: 130 1436 BstXI CCAn_nnnn'nTGG Cuts at: 0 1047 1566 Size: 1047 519 BstYI r'GATC_y Cuts at: 0 481 620 1566 Size: 481 139 946 Fragments arranged by size: 946 481 139 BtgI C'CryG_G Cuts at: 0 56 1155 1566 Size: 56 1099 411 Fragments arranged by size: 1099 411 56 BtsI GCAGTG_nn' Cuts at: 0 253 989 1566 Size: 253 736 577 Fragments arranged by size: 736 577 253 DrdI GACnn_nn'nnGTC Cuts at: 0 826 1566 Size: 826 740 EaeI y'GGCC_r Cuts at: 0 976 1175 1566 Size: 976 199 391 Fragments arranged by size: 976 391 199 EarI CTCTTCn'nnn_ Cuts at: 0 1039 1566 Size: 1039 527 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 873 1566 Size: 873 693 EcoICRI GAG'CTC Cuts at: 0 810 1566 Size: 810 756 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 403 475 613 826 1218 1514 1566 Size: 141 262 72 138 213 392 296 52 Fragments arranged by size: 392 296 262 213 141 138 72 52 EcoNI CCTnn'n_nnAGG Cuts at: 0 1117 1566 Size: 1117 449 EcoO109I rG'GnC_Cy Cuts at: 0 68 437 1338 1389 1566 Size: 68 369 901 51 177 Fragments arranged by size: 901 369 177 68 51 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 217 1218 1566 Size: 217 1001 348 Fragments arranged by size: 1001 348 217 HaeII r_GCGC'y Cuts at: 0 56 80 872 1376 1566 Size: 56 24 792 504 190 Fragments arranged by size: 792 504 190 56 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 755 787 1566 Size: 755 32 779 Fragments arranged by size: 779 755 32 HincII GTy'rAC Cuts at: 0 696 1566 Size: 696 870 KasI G'GCGC_C Cuts at: 0 76 1566 Size: 76 1490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 839 1566 Size: 839 727 MscI TGG'CCA Cuts at: 0 978 1566 Size: 978 588 MslI CAynn'nnrTG Cuts at: 0 535 1168 1566 Size: 535 633 398 Fragments arranged by size: 633 535 398 MspA1I CmG'CkG Cuts at: 0 36 58 1014 1566 Size: 36 22 956 552 Fragments arranged by size: 956 552 36 22 NaeI GCC'GGC Cuts at: 0 393 1126 1566 Size: 393 733 440 Fragments arranged by size: 733 440 393 NarI GG'CG_CC Cuts at: 0 77 1566 Size: 77 1489 NgoMIV G'CCGG_C Cuts at: 0 391 1124 1566 Size: 391 733 442 Fragments arranged by size: 733 442 391 PasI CC'CwG_GG Cuts at: 0 1258 1342 1566 Size: 1258 84 224 Fragments arranged by size: 1258 224 84 PflMI CCAn_nnn'nTGG Cuts at: 0 1014 1223 1566 Size: 1014 209 343 Fragments arranged by size: 1014 343 209 PfoI T'CCnGG_A Cuts at: 0 773 1566 Size: 773 793 PpuMI rG'GwC_Cy Cuts at: 0 1338 1566 Size: 1338 228 PstI C_TGCA'G Cuts at: 0 192 632 996 1121 1566 Size: 192 440 364 125 445 Fragments arranged by size: 445 440 364 192 125 PvuII CAG'CTG Cuts at: 0 36 1014 1566 Size: 36 978 552 Fragments arranged by size: 978 552 36 SacI G_AGCT'C Cuts at: 0 812 1566 Size: 812 754 SacII CC_GC'GG Cuts at: 0 59 1566 Size: 59 1507 SalI G'TCGA_C Cuts at: 0 694 1566 Size: 694 872 SanDI GG'GwC_CC Cuts at: 0 1338 1566 Size: 1338 228 SbfI CC_TGCA'GG Cuts at: 0 632 1121 1566 Size: 632 489 445 Fragments arranged by size: 632 489 445 SfcI C'TryA_G Cuts at: 0 188 628 992 1117 1566 Size: 188 440 364 125 449 Fragments arranged by size: 449 440 364 188 125 SfoI GGC'GCC Cuts at: 0 78 1566 Size: 78 1488 StuI AGG'CCT Cuts at: 0 1140 1432 1566 Size: 1140 292 134 Fragments arranged by size: 1140 292 134 StyI C'CwwG_G Cuts at: 0 1433 1566 Size: 1433 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 631 1398 1566 Size: 631 767 168 Fragments arranged by size: 767 631 168 TatI w'GTAC_w Cuts at: 0 539 1566 Size: 539 1027 Tth111I GACn'n_nGTC Cuts at: 0 769 1566 Size: 769 797 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 732 1566 Size: 732 834 XmnI GAAnn'nnTTC Cuts at: 0 578 1538 1566 Size: 578 960 28 Fragments arranged by size: 960 578 28 Enzymes that do cut: AccI AclI AcuI AflIII AlfI AlwNI ApoI AseI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI PpuMI PstI PvuII SacI SacII SalI SanDI SbfI SfcI SfoI StuI StyI TaqII TatI Tth111I XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AfeI AflII AgeI AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BciVI BclI BfrBI BglI BmtI BplI BpuEI BsaAI BsaBI BsiWI BsmI BspEI BsrBI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SapI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XhoI XmaI ZraI