Restriction map for
pFN21AE2591
##################################
# NO CUT ENZYMES ( 168 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BcnI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CfoI Cfr10I CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HaeII HaeIII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AflII 106 CTTAAG C^TTAAG
AspHI 990 GAGCTC G[AT]GC[AT]^C
AvaII 999 GGTCC G^G[AT]CC
BamHI 153 GGATCC G^GATCC
BbsI 187 GAAGAC GAAGAC(2/6)
BciVI 849 GGATAC GTATCC(6/5)
BclI 1563 TGATCA T^GATCA
BfrI 106 CTTAAG C^TTAAG
BlpI 3 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3 GCTAAGC GC^T[ACGT]AGC
BpuAI 187 GAAGAC GAAGAC(2/6)
BsaWI 1328 ACCGGA [AT]^CCGG[AT]
BsgI 169 GTGCAG GTGCAG(16/14)
BsiHKAI 990 GAGCTC G[AT]GC[AT]^C
BsiYI 1153 CCAAAGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 1153 CCAAAGACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspDI 1353 ATCGAT AT^CGAT
BstYI 153 GGATCC [GA]^GATC[CT]
CelII 3 GCTAAGC GC^T[ACGT]AGC
Cfr13I 999 GGTCC G^G[ACGT]CC
ClaI 1353 ATCGAT AT^CGAT
EcoT14I 164 CCTTGG C^C[AT][AT]GG
FbaI 1563 TGATCA T^GATCA
HapII 1329 CCGG C^CGG
HpaII 1329 CCGG C^CGG
MflI 153 GGATCC [GA]^GATC[CT]
MspI 1329 CCGG C^CGG
PstI 596 CTGCAG CTGCA^G
SacI 990 GAGCTC GAGCT^C
Sau96I 999 GGTCC G^G[ACGT]CC
ScaI 970 AGTACT AGT^ACT
StyI 164 CCTTGG C^C[AT][AT]GG
TaqI 1354 TCGA T^CGA
TthHB8I 1354 TCGA T^CGA
XhoII 153 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwI 154 GATCC GGATC(4/5)
153 GGATC
AlwNI 230 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
422 CAGTTGCTG
BanII 990 GAGCTC G[GA]GC[CT]^C
1425 GAGCCC
BmyI 990 GAGCTC G[AGT]GC[ACT]^C
1425 GAGCCC
BpmI 687 CTCCAG CTGGAG(16/14)
974 CTCCAG
BsaJI 164 CCTTGG C^C[ACGT][ACGT]GG
485 CCCTGG
BseRI 1610 CTCCTC GAGGAG(10/8)
625 GAGGAG
BsmFI 1169 GGGAC GGGAC(10/14)
948 GTCCC
Bsp1286I 990 GAGCTC G[AGT]GC[ACT]^C
1425 GAGCCC
BssKI 486 CCTGG ^CC[ACGT]GG
673 CCTGG
BstAPI 421 GCAGTTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
589 GCACTTTCTGC
BstNI 486 CCTGG CC^[AT]GG
673 CCTGG
EcoRI 570 GAATTC G^AATTC
1302 GAATTC
EcoRII 486 CCTGG ^CC[AT]GG
673 CCTGG
MvaI 486 CCTGG CC^[AT]GG
673 CCTGG
PleI 130 GAGTC GAGTC(4/5)
1408 GAGTC
SapI 100 GAAGAGC GCTCTTC(1/4)
430 GCTCTTC
ScrFI 486 CCTGG CC^[ACGT]GG
673 CCTGG
SfcI 236 CTGTAG C^T[GA][CT]AG
596 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BsrI BstF5I DdeI DpnII
EarI Fnu4HI FokI HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MnlI MseI MwoI NdeII
NlaIV RsaI Sau3AI SfaNI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI