Restriction map for
pFN21AE2591
(Circular) (Six-base) MAPSORT of: pFN21AE2591.seq Check: 9280 from: 1 to: 6328
pFN21AE2591 6328 bp
With 182 enzymes: SgfI *
October 13, 2015 18:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4138 278
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3671 1937
Size: 1734 4594
Acc65I G'GTAC_C
Cuts at: 3649 3649
Size: 6328
AclI AA'CG_TT
Cuts at: 1563 4455 4828 1563
Size: 2892 373 3063
Fragments arranged by size:
3063 2892 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2399 2406 2423 2951 3084 4391 5775 6043
Size: 767 7 17 528 133 1307 1384 268
Cuts at: 6043 1632
Size: 1917
Fragments arranged by size:
1917 1384 1307 767 528 268 133 17
7
AflII C'TTAA_G
Cuts at: 820 1017 2111 820
Size: 197 1094 5037
Fragments arranged by size:
5037 1094 197
AflIII A'CryG_T
Cuts at: 1203 5227 6204 1203
Size: 4024 977 1327
Fragments arranged by size:
4024 1327 977
AgeI A'CCGG_T
Cuts at: 5159 5159
Size: 6328
AhdI GACnn_n'nnGTC
Cuts at: 5057 5057
Size: 6328
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6328
AlwNI CAG_nnn'CTG
Cuts at: 2240 2432 4071 5643 2240
Size: 192 1639 1572 2925
Fragments arranged by size:
2925 1639 1572 192
ApaLI G'TGCA_C
Cuts at: 4385 5541 4385
Size: 1156 5172
ApoI r'AATT_y
Cuts at: 1493 2368 2548 2575 3241 3307 3497 3637 3863
Size: 875 180 27 666 66 190 140 226
Cuts at: 3863 4015 6124 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 875 666 226 190 180 152
140 66 27
AseI AT'TA_AT
Cuts at: 160 1032 4882 160
Size: 872 3850 1606
Fragments arranged by size:
3850 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
AvaI C'yCGr_G
Cuts at: 1870 1943 3653 1870
Size: 73 1710 4545
Fragments arranged by size:
4545 1710 73
BamHI G'GATC_C
Cuts at: 2158 3658 4025 2158
Size: 1500 367 4461
Fragments arranged by size:
4461 1500 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3649 5104 618
Size: 325 217 627 1862 1455 1842
Fragments arranged by size:
1862 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2999 3434 3647 1391
Size: 1608 435 213 4072
Fragments arranged by size:
4072 1608 435 213
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2199 928
Size: 971 300 5057
Fragments arranged by size:
5057 971 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4519 4553 1903
Size: 34 50 34 2498 34 3678
Fragments arranged by size:
3678 2498 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2848 4220 5430 2848
Size: 1372 1210 3746
Fragments arranged by size:
3746 1372 1210
BclI T'GATC_A
Cuts at: 1548 3568 1548
Size: 2020 4308
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4939 136
Size: 107 122 71 631 605 3267 1525
Fragments arranged by size:
3267 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6323 6323
Size: 6328
BlpI GC'TnA_GC
Cuts at: 2009 3745 2009
Size: 1736 4592
Bme1580I G_kGCm'C
Cuts at: 1790 4389 5545 1790
Size: 2599 1156 2573
Fragments arranged by size:
2599 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4059 5017 442
Size: 416 53 83 399 24 2642 958 1753
Fragments arranged by size:
2642 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6328
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2677 2964 4988 1239
Size: 1438 287 2024 2579
Fragments arranged by size:
2579 2024 1438 287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2465 2514 3471 3524 4453 5318 5616 2465
Size: 49 957 53 929 865 298 3177
Fragments arranged by size:
3177 957 929 865 298 53 49
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4991 882
Size: 617 3492 2219
Fragments arranged by size:
3492 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4119 493
Size: 711 2915 2702
Fragments arranged by size:
2915 2702 711
BsaBI GATnn'nnATC
Cuts at: 6322 6322
Size: 6328
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4135 4517 5152 275
Size: 53 83 186 936 2602 382 635 1451
Fragments arranged by size:
2602 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3333 4761 5159 5433 5580 5991 3333
Size: 1428 398 274 147 411 3670
Fragments arranged by size:
3670 1428 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6103
Fragments arranged by size:
6103 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2645 3606 1186
Size: 1459 961 3908
Fragments arranged by size:
3908 1459 961
BseYI C'CCAG_C
Cuts at: 5531 6027 5531
Size: 496 5832
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2195 1163
Size: 573 459 5296
Fragments arranged by size:
5296 573 459
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3778 4539 5567 1540
Size: 275 186 1777 761 1028 2301
Fragments arranged by size:
2301 1777 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2999 4389 4474 5545 2999
Size: 1390 85 1071 3782
Fragments arranged by size:
3782 1390 1071 85
BsmI GAATG_Cn'
Cuts at: 3839 3932 3839
Size: 93 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2999 3434 3647 4389 4474 5545 1391
Size: 399 1209 435 213 742 85 1071 2174
Fragments arranged by size:
2174 1209 1071 742 435 399 213 85
BspEI T'CCGG_A
Cuts at: 5991 5991
Size: 6328
BspHI T'CATG_A
Cuts at: 4217 5947 4217
Size: 1730 4598
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3683 844
Size: 2839 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4215 1394
Size: 429 2392 3507
Fragments arranged by size:
3507 2392 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4823 4997 65
Size: 1183 3575 174 1396
Fragments arranged by size:
3575 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4972 5159 1700
Size: 110 3162 187 2869
Fragments arranged by size:
3162 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6328
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6328
BssSI C'ACGA_G
Cuts at: 4388 5400 4388
Size: 1012 5316
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2432 2600 794
Size: 1638 168 4522
Fragments arranged by size:
4522 1638 168
BstBI TT'CG_AA
Cuts at: 5132 5132
Size: 6328
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6328
BstYI r'GATC_y
Cuts at: 1972 2158 3658 4025 4410 4427 5879 5965 5977
Size: 186 1500 367 385 17 1452 86 12
Cuts at: 5977 6323 1972
Size: 346 1977
Fragments arranged by size:
1977 1500 1452 385 367 346 186 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5776
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5589
Fragments arranged by size:
5589 683 56
BtsI GCAGTG_nn'
Cuts at: 2632 3499 3844 4638 4658 2632
Size: 867 345 794 20 4302
Fragments arranged by size:
4302 867 794 345 20
ClaI AT'CG_AT
Cuts at: 1554 3359 1554
Size: 1805 4523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6293
DraI TTT'AAA
Cuts at: 3632 3632
Size: 6328
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5335 809
Size: 507 4019 1802
Fragments arranged by size:
4019 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3775 4664 6293
Size: 54 1425 50 275 18 1945 889 1629
Cuts at: 6293 8
Size: 43
Fragments arranged by size:
1945 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3775 1537
Size: 275 1963 4090
Fragments arranged by size:
4090 1963 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2100 2442 2553 4258 1353
Size: 747 342 111 1705 3423
Fragments arranged by size:
3423 1705 747 342 111
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4915 5289 5435 387
Size: 1064 138 3326 374 146 1280
Fragments arranged by size:
3326 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2997 2997
Size: 6328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2399 2406 2423 2677 2951 2964 3084
Size: 393 767 7 17 254 274 13 120
Cuts at: 3084 4391 4988 5775 6043 1239
Size: 1307 597 787 268 1524
Fragments arranged by size:
1524 1307 787 767 597 393 274 268
254 120 17 13 7
EcoRI G'AATT_C
Cuts at: 2575 3307 3637 4015 2575
Size: 732 330 378 4888
Fragments arranged by size:
4888 732 378 330
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2795 2795
Size: 6328
FspI TGC'GCA
Cuts at: 4834 4834
Size: 6328
HaeII r_GCGC'y
Cuts at: 1678 5475 1678
Size: 3797 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2107 2139 2384 2416 2627
Size: 32 135 32 535 32 245 32 211
Cuts at: 2627 2659 3371 3403 3458 3490 4969 5001 5043
Size: 32 712 32 55 32 1479 32 42
Cuts at: 5043 5075 1373
Size: 32 2626
Fragments arranged by size:
2626 1479 712 535 245 211 135 55
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3672 3918 669
Size: 988 281 1734 246 3079
Fragments arranged by size:
3079 1734 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6328
HpaI GTT'AAC
Cuts at: 3918 3918
Size: 6328
KpnI G_GTAC'C
Cuts at: 3653 3653
Size: 6328
MfeI C'AATT_G
Cuts at: 3927 3927
Size: 6328
MluI A'CGCG_T
Cuts at: 6204 6204
Size: 6328
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3229 3375 3501 5442 5626 1305
Size: 1924 146 126 1941 184 2007
Fragments arranged by size:
2007 1941 1924 184 146 126
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4506
Fragments arranged by size:
4506 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4286 4645 4804 518
Size: 648 3120 359 159 2042
Fragments arranged by size:
3120 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3744 4421 5569 5814 1606
Size: 2138 677 1148 245 2120
Fragments arranged by size:
2138 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5776
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6328
NotI GC'GGCC_GC
Cuts at: 3775 3775
Size: 6328
NspI r_CATG'y
Cuts at: 1132 1229 3686 5231 1132
Size: 97 2457 1545 2229
Fragments arranged by size:
2457 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6328
PciI A'CATG_T
Cuts at: 5227 5227
Size: 6328
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6328
PmeI GTTT'AAAC
Cuts at: 3632 3632
Size: 6328
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4397 4429 5910 5942 4397
Size: 32 1481 32 4783
Fragments arranged by size:
4783 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6328
PsiI TTA'TAA
Cuts at: 3898 3898
Size: 6328
PstI C_TGCA'G
Cuts at: 830 2605 3680 830
Size: 1775 1075 3478
Fragments arranged by size:
3478 1775 1075
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6328
SacI G_AGCT'C
Cuts at: 2999 2999
Size: 6328
SalI G'TCGA_C
Cuts at: 1936 3670 1936
Size: 1734 4594
SapI GCTCTTCn'nnn_
Cuts at: 2100 2442 2100
Size: 342 5986
SbfI CC_TGCA'GG
Cuts at: 3680 3680
Size: 6328
ScaI AGT'ACT
Cuts at: 2977 4576 6288 2977
Size: 1599 1712 3017
Fragments arranged by size:
3017 1712 1599
SfcI C'TryA_G
Cuts at: 826 1045 2241 2601 3676 4811 5492 5683 826
Size: 219 1196 360 1075 1135 681 191 1471
Fragments arranged by size:
1471 1196 1135 1075 681 360 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6328
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6328
SmaI CCC'GGG
Cuts at: 1872 3655 1872
Size: 1783 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2111 2480 2529 3486 3503 4432
Size: 197 926 168 369 49 957 17 929
Cuts at: 4432 5333 5595 820
Size: 901 262 1553
Fragments arranged by size:
1553 957 929 926 901 369 262 197
168 49 17
SnaBI TAC'GTA
Cuts at: 493 4119 493
Size: 3626 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6328
SphI G_CATG'C
Cuts at: 1132 3686 1132
Size: 2554 3774
SspI AAT'ATT
Cuts at: 5 52 4252 5
Size: 47 4200 2081
Fragments arranged by size:
4200 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6328
StyI C'CwwG_G
Cuts at: 513 1065 2169 3767 513
Size: 552 1104 1598 3074
Fragments arranged by size:
3074 1598 1104 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4355 4372 4525 4710 5155 325
Size: 1255 2775 17 153 185 445 1498
Fragments arranged by size:
2775 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2942 2975 4574
Size: 274 80 33 51 1443 965 33 1599
Cuts at: 4574 6286 96
Size: 1712 138
Fragments arranged by size:
1712 1599 1443 965 274 138 80 51
33 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2200 2232 6288 6320 2200
Size: 32 4056 32 2208
Fragments arranged by size:
4056 2208 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6328
XbaI T'CTAG_A
Cuts at: 3664 3664
Size: 6328
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6328
XmaI C'CCGG_G
Cuts at: 1870 3653 1870
Size: 1783 4545
XmnI GAAnn'nnTTC
Cuts at: 4457 4457
Size: 6328
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4136 276
Size: 53 83 186 936 2602 2468
Fragments arranged by size:
2602 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI