(Linear) (Six-base) MAPSORT of: KIEE2591.seq Check: 5711 from: 1 to: 1623 KIEE2591.orf With 182 enzymes: SgfI * October 13, 2015 18:22 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 401 418 946 1079 1623 Size: 394 7 17 528 133 544 Fragments arranged by size: 544 528 394 133 17 7 AflII C'TTAA_G Cuts at: 0 106 1623 Size: 106 1517 AlwNI CAG_nnn'CTG Cuts at: 0 235 427 1623 Size: 235 192 1196 Fragments arranged by size: 1196 235 192 ApoI r'AATT_y Cuts at: 0 363 543 570 1236 1302 1492 1623 Size: 363 180 27 666 66 190 131 Fragments arranged by size: 666 363 190 180 131 66 27 BamHI G'GATC_C Cuts at: 0 153 1623 Size: 153 1470 BanII G_rGCy'C Cuts at: 0 994 1429 1623 Size: 994 435 194 Fragments arranged by size: 994 435 194 BbsI GAAGACnn'nnnn_ Cuts at: 0 194 1623 Size: 194 1429 BciVI GTATCCnnnnn_n' Cuts at: 0 843 1623 Size: 843 780 BclI T'GATC_A Cuts at: 0 1563 1623 Size: 1563 60 BlpI GC'TnA_GC Cuts at: 0 4 1623 Size: 4 1619 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 672 959 1623 Size: 672 287 664 Fragments arranged by size: 672 664 287 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 460 509 1466 1519 1623 Size: 460 49 957 53 104 Fragments arranged by size: 957 460 104 53 49 BsaWI w'CCGG_w Cuts at: 0 1328 1623 Size: 1328 295 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 640 1601 1623 Size: 640 961 22 Fragments arranged by size: 961 640 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 190 1623 Size: 190 1433 BsiHKAI G_wGCw'C Cuts at: 0 994 1623 Size: 994 629 Bsp1286I G_dGCh'C Cuts at: 0 994 1429 1623 Size: 994 435 194 Fragments arranged by size: 994 435 194 BstAPI GCAn_nnn'nTGC Cuts at: 0 427 595 1623 Size: 427 168 1028 Fragments arranged by size: 1028 427 168 BstYI r'GATC_y Cuts at: 0 153 1623 Size: 153 1470 BtsI GCAGTG_nn' Cuts at: 0 627 1494 1623 Size: 627 867 129 Fragments arranged by size: 867 627 129 ClaI AT'CG_AT Cuts at: 0 1354 1623 Size: 1354 269 EarI CTCTTCn'nnn_ Cuts at: 0 95 437 548 1623 Size: 95 342 111 1075 Fragments arranged by size: 1075 342 111 95 EcoICRI GAG'CTC Cuts at: 0 992 1623 Size: 992 631 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 401 418 672 946 959 1079 1623 Size: 394 7 17 254 274 13 120 544 Fragments arranged by size: 544 394 274 254 120 17 13 7 EcoRI G'AATT_C Cuts at: 0 570 1302 1623 Size: 570 732 321 Fragments arranged by size: 732 570 321 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 790 1623 Size: 790 833 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 102 134 379 411 622 654 1366 1398 Size: 102 32 245 32 211 32 712 32 Cuts at: 1398 1453 1485 1623 Size: 55 32 138 Fragments arranged by size: 712 245 211 138 102 55 32 32 32 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1224 1370 1496 1623 Size: 1224 146 126 127 Fragments arranged by size: 1224 146 127 126 PstI C_TGCA'G Cuts at: 0 600 1623 Size: 600 1023 SacI G_AGCT'C Cuts at: 0 994 1623 Size: 994 629 SapI GCTCTTCn'nnn_ Cuts at: 0 95 437 1623 Size: 95 342 1186 Fragments arranged by size: 1186 342 95 ScaI AGT'ACT Cuts at: 0 972 1623 Size: 972 651 SfcI C'TryA_G Cuts at: 0 236 596 1623 Size: 236 360 1027 Fragments arranged by size: 1027 360 236 SmlI C'TyrA_G Cuts at: 0 106 475 524 1481 1498 1623 Size: 106 369 49 957 17 125 Fragments arranged by size: 957 369 125 106 49 17 StyI C'CwwG_G Cuts at: 0 164 1623 Size: 164 1459 TatI w'GTAC_w Cuts at: 0 937 970 1623 Size: 937 33 653 Fragments arranged by size: 937 653 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 195 227 1623 Size: 195 32 1396 Fragments arranged by size: 1396 195 32 Enzymes that do cut: AcuI AflII AlwNI ApoI BamHI BanII BbsI BciVI BclI BlpI BpmI BpuEI BsaWI BseRI BsgI BsiHKAI Bsp1286I BstAPI BstYI BtsI ClaI EarI EcoICRI Eco57MI EcoRI FalI Hin4I MmeI PstI SacI SapI ScaI SfcI SmlI StyI TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI BcgI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI CspCI DraI DraIII DrdI EaeI EagI EciI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI