(Circular) (Six-base) MAPSORT of: pFN21AE6479.seq Check: 4086 from: 1 to: 6130 pFN21AE6479 6130 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 2934 3451 2934 Size: 517 5613 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3075 3154 3443 4193 5577 5845 1632 Size: 1443 79 289 750 1384 268 1917 Fragments arranged by size: 1917 1443 1384 750 289 268 79 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 5029 6006 1203 Size: 3826 977 1327 Fragments arranged by size: 3826 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 2357 1166 Size: 1191 4939 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 2513 3873 5445 2513 Size: 1360 1572 3198 Fragments arranged by size: 3198 1572 1360 ApaI G_GGCC'C Cuts at: 2411 2411 Size: 6130 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3439 3665 3817 5926 1493 Size: 1946 226 152 2109 1697 Fragments arranged by size: 2109 1946 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 3455 1870 Size: 73 1512 4545 Fragments arranged by size: 4545 1512 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2298 2934 2966 3451 4906 Size: 325 217 627 511 636 32 485 1455 Cuts at: 4906 618 Size: 1842 Fragments arranged by size: 1842 1455 636 627 511 485 325 217 32 BanII G_rGCy'C Cuts at: 1391 2177 2264 2349 2411 2708 2750 3337 3449 Size: 786 87 85 62 297 42 587 112 Cuts at: 3449 1391 Size: 4072 Fragments arranged by size: 4072 786 587 297 112 87 85 62 42 BbeI G_GCGC'C Cuts at: 2970 2970 Size: 6130 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2285 3215 928 Size: 971 386 930 3843 Fragments arranged by size: 3843 971 930 386 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2493 2637 Size: 107 122 71 631 605 337 484 144 Cuts at: 2637 4741 136 Size: 2104 1525 Fragments arranged by size: 2104 1525 631 605 484 337 144 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2301 2411 4191 5347 1790 Size: 511 110 1780 1156 2573 Fragments arranged by size: 2573 1780 1156 511 110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3861 4819 442 Size: 416 53 83 399 24 2444 958 1753 Fragments arranged by size: 2444 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2527 2527 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2345 4790 1239 Size: 1106 2445 2579 Fragments arranged by size: 2579 2445 1106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4255 5120 5418 4255 Size: 865 298 4967 Fragments arranged by size: 4967 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3166 3418 4793 882 Size: 617 1667 252 1375 2219 Fragments arranged by size: 2219 1667 1375 617 252 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 2043 6124 2043 Size: 4081 2049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2967 3937 4319 4954 Size: 53 83 186 936 1434 970 382 635 Cuts at: 4954 275 Size: 1451 Fragments arranged by size: 1451 1434 970 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2193 2223 1178 Size: 30 165 30 790 30 5085 Fragments arranged by size: 5085 790 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 2834 2873 1186 Size: 1330 318 39 4443 Fragments arranged by size: 4443 1330 318 39 BseYI C'CCAG_C Cuts at: 2257 2334 2599 3191 3417 5333 5829 2257 Size: 77 265 592 226 1916 496 2558 Fragments arranged by size: 2558 1916 592 496 265 226 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2330 2689 2879 1163 Size: 573 594 359 190 4414 Fragments arranged by size: 4414 594 573 359 190 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2826 4191 4276 5347 2826 Size: 1365 85 1071 3609 Fragments arranged by size: 3609 1365 1071 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 BsmBI CGTCTCn'nnnn_ Cuts at: 2361 2946 2361 Size: 585 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2264 2301 2349 2411 2708 2750 Size: 399 387 87 37 48 62 297 42 Cuts at: 2750 2826 3337 3449 4191 4276 5347 1391 Size: 76 511 112 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 511 399 387 297 112 87 85 76 62 48 42 37 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3061 3485 844 Size: 2217 424 3489 Fragments arranged by size: 3489 2217 424 BsrBI CCG'CTC Cuts at: 1394 1823 2233 4017 1394 Size: 429 410 1784 3507 Fragments arranged by size: 3507 1784 429 410 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2417 4774 4961 1700 Size: 110 607 2357 187 2869 Fragments arranged by size: 2869 2357 607 187 110 BsrGI T'GTAC_A Cuts at: 96 3132 96 Size: 3036 3094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 3035 4190 5202 3035 Size: 1155 1012 3963 Fragments arranged by size: 3963 1155 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstXI CCAn_nnnn'nTGG Cuts at: 2875 3010 3391 2875 Size: 135 381 5614 Fragments arranged by size: 5614 381 135 BstYI r'GATC_y Cuts at: 1972 2226 2886 3460 3827 4212 4229 5681 5767 Size: 254 660 574 367 385 17 1452 86 Cuts at: 5767 5779 6125 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 660 574 385 367 346 254 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2633 2633 Size: 6130 BtgI C'CryG_G Cuts at: 513 1065 2004 2799 2953 3142 3285 513 Size: 552 939 795 154 189 143 3358 Fragments arranged by size: 3358 939 795 552 189 154 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 3646 4440 4460 3646 Size: 794 20 5316 Fragments arranged by size: 5316 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2061 2096 529 Size: 35 1497 35 4563 Fragments arranged by size: 4563 1497 35 35 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2105 2415 2802 Size: 54 1425 50 275 18 275 310 387 Cuts at: 2802 3393 3577 4466 6095 8 Size: 591 184 889 1629 43 Fragments arranged by size: 1629 1425 889 591 387 310 275 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2108 2811 3193 4060 1353 Size: 755 703 382 867 3423 Fragments arranged by size: 3423 867 755 703 382 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 3075 3154 3443 4193 4790 5577 Size: 393 713 730 79 289 750 597 787 Cuts at: 5577 5845 1239 Size: 268 1524 Fragments arranged by size: 1524 787 750 730 713 597 393 289 268 79 EcoNI CCTnn'n_nnAGG Cuts at: 3407 3407 Size: 6130 EcoO109I rG'GnC_Cy Cuts at: 2407 2518 2407 Size: 111 6019 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 EcoRV GAT'ATC Cuts at: 2194 2194 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 2970 5277 1678 Size: 1292 2307 2531 Fragments arranged by size: 2531 2307 1292 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2109 2141 2472 2504 4771 Size: 32 135 32 537 32 331 32 2267 Cuts at: 4771 4803 4845 4877 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2267 537 331 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2473 3474 3720 669 Size: 988 281 535 1001 246 3079 Fragments arranged by size: 3079 1001 988 535 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KasI G'GCGC_C Cuts at: 2966 2966 Size: 6130 KpnI G_GTAC'C Cuts at: 2938 3455 2938 Size: 517 5613 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2289 3189 5244 5428 1305 Size: 984 900 2055 184 2007 Fragments arranged by size: 2055 2007 984 900 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2304 2357 2660 2918 4088 4447 4606 Size: 648 1138 53 303 258 1170 359 159 Cuts at: 4606 518 Size: 2042 Fragments arranged by size: 2042 1170 1138 648 359 303 258 159 53 MspA1I CmG'CkG Cuts at: 1606 3323 3421 3546 4223 5371 5616 1606 Size: 1717 98 125 677 1148 245 2120 Fragments arranged by size: 2120 1717 1148 677 245 125 98 NaeI GCC'GGC Cuts at: 2419 2419 Size: 6130 NarI GG'CG_CC Cuts at: 2967 2967 Size: 6130 NcoI C'CATG_G Cuts at: 513 1065 2004 2953 513 Size: 552 939 949 3690 Fragments arranged by size: 3690 949 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NgoMIV G'CCGG_C Cuts at: 2417 2417 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 3488 5033 1132 Size: 97 2259 1545 2229 Fragments arranged by size: 2259 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3156 1348 Size: 1808 4322 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3171 1613 Size: 1558 4572 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PspOMI G'GGCC_C Cuts at: 2407 2407 Size: 6130 PstI C_TGCA'G Cuts at: 830 2155 2320 3482 830 Size: 1325 165 1162 3478 Fragments arranged by size: 3478 1325 1162 165 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 3421 3421 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SapI GCTCTTCn'nnn_ Cuts at: 3193 3193 Size: 6130 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2151 2316 3478 4613 5294 5485 826 Size: 219 1106 165 1162 1135 681 191 1471 Fragments arranged by size: 1471 1162 1135 1106 681 219 191 165 SfoI GGC'GCC Cuts at: 2968 2968 Size: 6130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4234 5135 5397 820 Size: 197 926 2291 901 262 1553 Fragments arranged by size: 2291 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2004 2868 2953 3569 513 Size: 552 939 864 85 616 3074 Fragments arranged by size: 3074 939 864 616 552 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2068 4157 4174 4327 4512 4957 325 Size: 1255 488 2089 17 153 185 445 1498 Fragments arranged by size: 2089 1498 1255 488 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 3132 4376 Size: 274 80 33 51 1443 54 1101 1244 Cuts at: 4376 6088 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1244 1101 274 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 2573 1316 Size: 1257 4873 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI