Restriction map for
pFN21AE5656
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvaI AviII
AvrII BbeI BbrPI BbvCI BcgI
BcnI BfrI BglII BlnI BsaAI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRI EcoRV EcoT22I
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI MamI
MfeI MluI MroI MunI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RsrII SalI SbfI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccII 1180 CGCG CG^CG
AcsI 556 AAATTT [GA]^AATT[CT]
AflIII 1169 ACGTGT A^C[GA][CT]GT
Aor51HI 1127 AGCGCT AGC^GCT
ApaI 17 GGGCCC GGGCC^C
ApoI 556 AAATTT [GA]^AATT[CT]
Asp700I 280 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2 TGGCCA TGG^CCA
BamHI 243 GGATCC G^GATCC
BanI 643 GGCACC G^G[CT][GA]CC
BbsI 829 GAAGAC GAAGAC(2/6)
BciVI 909 GGATAC GTATCC(6/5)
BclI 191 TGATCA T^GATCA
BfaI 1034 CTAG C^TAG
BglI 1289 GCCGCTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 453 GCTCAGC GC^T[ACGT]AGC
BpmI 307 CTCCAG CTGGAG(16/14)
Bpu1102I 453 GCTCAGC GC^T[ACGT]AGC
BpuAI 829 GAAGAC GAAGAC(2/6)
BsaWI 1271 ACCGGA [AT]^CCGG[AT]
BseRI 148 GAGGAG GAGGAG(10/8)
BsmBI 947 GAGACG CGTCTC(1/5)
BspHI 734 TCATGA T^CATGA
BspMI 1265 ACCTGC ACCTGC(4/8)
BsrDI 993 CATTGC GCAATG(2/0)
BssSI 12 CTCGTG CACGAG(-5/-1)
BstEII 1158 GGTGACC G^GT[ACGT]ACC
BstPI 1158 GGTGACC G^GT[ACGT]ACC
BstUI 1180 CGCG CG^CG
BstYI 243 GGATCC [GA]^GATC[CT]
CelII 453 GCTCAGC GC^T[ACGT]AGC
DraIII 1069 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 2 TGGCCA [CT]^GGCC[GA]
Eco47III 1127 AGCGCT AGC^GCT
EcoO65I 1158 GGTGACC G^GT[ACGT]ACC
EcoT14I 323 CCATGG C^C[AT][AT]GG
FauI 1178 CCCGC CCCGC(4/6)
FbaI 191 TGATCA T^GATCA
HapII 1272 CCGG C^CGG
HindIII 574 AAGCTT A^AGCTT
HpaII 1272 CCGG C^CGG
KspI 1179 CCGCGG CCGC^GG
MaeI 1034 CTAG C^TAG
MaeII 1169 ACGT A^CGT
MflI 243 GGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MseI 1373 TTAA T^TAA
MslI 1164 CACCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1272 CCGG C^CGG
MvnI 1180 CGCG CG^CG
NcoI 323 CCATGG C^CATGG
NdeI 509 CATATG CA^TATG
PvuII 431 CAGCTG CAG^CTG
RcaI 734 TCATGA T^CATGA
SacI 1051 GAGCTC GAGCT^C
SacII 1179 CCGCGG CCGC^GG
SapI 280 GAAGAGC GCTCTTC(1/4)
ScaI 923 AGTACT AGT^ACT
SexAI 1070 ACCAGGT A^CC[AT]GGT
StuI 962 AGGCCT AGG^CCT
StyI 323 CCATGG C^C[AT][AT]GG
Tru9I 1373 TTAA T^TAA
Tsp45I 1159 GTGAC ^GT[GC]AC
XcmI 405 CCAGTCTCAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 243 GGATCC [GA]^GATC[CT]
XmnI 280 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
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AspHI 669 GTGCTC G[AT]GC[AT]^C
1051 GAGCTC
AvaII 653 GGACC G^G[AT]CC
861 GGTCC
BanII 17 GGGCCC G[GA]GC[CT]^C
1051 GAGCTC
BsaI 901 GAGACC GGTCTC(1/5)
1258 GAGACC
BsgI 1267 CTGCAC GTGCAG(16/14)
936 GTGCAG
BsiHKAI 669 GTGCTC G[AT]GC[AT]^C
1051 GAGCTC
BsmFI 652 GGGAC GGGAC(10/14)
862 GTCCC
DraII 17 GGGCCCT [GA]G^G[ACGT]CC[CT]
1304 GGGGCCT
EcoO109I 17 GGGCCCT [GA]G^G[ACGT]CC[CT]
1304 GGGGCCT
HaeII 519 GGCGCT [GA]GCGC^[CT]
1127 AGCGCT
MaeIII 971 GTAAC ^GT[ACGT]AC
1159 GTGAC
MspA1I 431 CAGCTG C[AC]G^C[GT]G
1179 CCGCGG
PflMI 852 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1068 CCACCAGGTGG
SmlI 679 CTCAAG C^T[CT][GA]AG
998 CTCAAG
Van91I 852 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1068 CCACCAGGTGG
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbvI BmyI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI TaqI TfiI TseI
Tsp509I TspRI TthHB8I