(Linear) (Six-base) MAPSORT of: KIEE5656.seq Check: 9194 from: 1 to: 1434 KIEE5656.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 592 907 1434 Size: 592 315 527 Fragments arranged by size: 592 527 315 AfeI AGC'GCT Cuts at: 0 1129 1434 Size: 1129 305 AflIII A'CryG_T Cuts at: 0 1169 1434 Size: 1169 265 AleI CACnn'nnGTG Cuts at: 0 1168 1434 Size: 1168 266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1130 1434 Size: 1130 304 AlwNI CAG_nnn'CTG Cuts at: 0 396 492 797 807 1434 Size: 396 96 305 10 627 Fragments arranged by size: 627 396 305 96 10 ApaI G_GGCC'C Cuts at: 0 21 1434 Size: 21 1413 ApoI r'AATT_y Cuts at: 0 556 1434 Size: 556 878 BamHI G'GATC_C Cuts at: 0 243 1434 Size: 243 1191 BanI G'GyrC_C Cuts at: 0 643 1434 Size: 643 791 BanII G_rGCy'C Cuts at: 0 21 1055 1434 Size: 21 1034 379 Fragments arranged by size: 1034 379 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 836 1434 Size: 836 598 BciVI GTATCCnnnnn_n' Cuts at: 0 903 1434 Size: 903 531 BclI T'GATC_A Cuts at: 0 191 1434 Size: 191 1243 BglI GCCn_nnn'nGGC Cuts at: 0 1295 1434 Size: 1295 139 BlpI GC'TnA_GC Cuts at: 0 454 1434 Size: 454 980 Bme1580I G_kGCm'C Cuts at: 0 21 1179 1434 Size: 21 1158 255 Fragments arranged by size: 1158 255 21 BmrI ACTGGGnnnn_n' Cuts at: 0 165 399 480 1434 Size: 165 234 81 954 Fragments arranged by size: 954 234 165 81 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 1434 Size: 292 1142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 664 983 1434 Size: 664 319 451 Fragments arranged by size: 664 451 319 BsaI GGTCTCn'nnnn_ Cuts at: 0 895 1252 1434 Size: 895 357 182 Fragments arranged by size: 895 357 182 BsaWI w'CCGG_w Cuts at: 0 1271 1434 Size: 1271 163 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 290 320 655 685 1434 Size: 290 30 335 30 749 Fragments arranged by size: 749 335 290 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 163 1434 Size: 163 1271 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 957 1252 1434 Size: 957 295 182 Fragments arranged by size: 957 295 182 BsiHKAI G_wGCw'C Cuts at: 0 673 1055 1434 Size: 673 382 379 Fragments arranged by size: 673 382 379 BsmBI CGTCTCn'nnnn_ Cuts at: 0 941 1434 Size: 941 493 Bsp1286I G_dGCh'C Cuts at: 0 21 673 1055 1179 1434 Size: 21 652 382 124 255 Fragments arranged by size: 652 382 255 124 21 BspHI T'CATG_A Cuts at: 0 734 1434 Size: 734 700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1274 1434 Size: 1274 160 BsrDI GCAATG_nn' Cuts at: 0 992 1434 Size: 992 442 BssSI C'ACGA_G Cuts at: 0 12 1434 Size: 12 1422 BstEII G'GTnAC_C Cuts at: 0 1158 1434 Size: 1158 276 BstYI r'GATC_y Cuts at: 0 243 1434 Size: 243 1191 BtgI C'CryG_G Cuts at: 0 323 1179 1229 1434 Size: 323 856 50 205 Fragments arranged by size: 856 323 205 50 BtsI GCAGTG_nn' Cuts at: 0 125 1319 1434 Size: 125 1194 115 Fragments arranged by size: 1194 125 115 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 206 241 1434 Size: 206 35 1193 Fragments arranged by size: 1193 206 35 DraIII CAC_nnn'GTG Cuts at: 0 1074 1434 Size: 1074 360 EaeI y'GGCC_r Cuts at: 0 2 1434 Size: 2 1432 EarI CTCTTCn'nnn_ Cuts at: 0 74 140 275 893 1434 Size: 74 66 135 618 541 Fragments arranged by size: 618 541 135 74 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 807 1434 Size: 807 627 EcoICRI GAG'CTC Cuts at: 0 1053 1434 Size: 1053 381 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 292 592 907 1434 Size: 292 300 315 527 Fragments arranged by size: 527 315 300 292 EcoO109I rG'GnC_Cy Cuts at: 0 18 1305 1434 Size: 18 1287 129 Fragments arranged by size: 1287 129 18 HaeII r_GCGC'y Cuts at: 0 523 1131 1434 Size: 523 608 303 Fragments arranged by size: 608 523 303 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 235 267 655 687 729 761 1434 Size: 235 32 388 32 42 32 673 Fragments arranged by size: 673 388 235 42 32 32 32 HindIII A'AGCT_T Cuts at: 0 574 1434 Size: 574 860 MscI TGG'CCA Cuts at: 0 4 1434 Size: 4 1430 MslI CAynn'nnrTG Cuts at: 0 1168 1434 Size: 1168 266 MspA1I CmG'CkG Cuts at: 0 433 1181 1434 Size: 433 748 253 Fragments arranged by size: 748 433 253 NcoI C'CATG_G Cuts at: 0 323 1434 Size: 323 1111 NdeI CA'TA_TG Cuts at: 0 510 1434 Size: 510 924 PasI CC'CwG_GG Cuts at: 0 649 1434 Size: 649 785 PflMI CCAn_nnn'nTGG Cuts at: 0 858 1074 1434 Size: 858 216 360 Fragments arranged by size: 858 360 216 PfoI T'CCnGG_A Cuts at: 0 1361 1434 Size: 1361 73 PspOMI G'GGCC_C Cuts at: 0 17 1434 Size: 17 1417 PvuII CAG'CTG Cuts at: 0 433 1434 Size: 433 1001 SacI G_AGCT'C Cuts at: 0 1055 1434 Size: 1055 379 SacII CC_GC'GG Cuts at: 0 1182 1434 Size: 1182 252 SapI GCTCTTCn'nnn_ Cuts at: 0 275 1434 Size: 275 1159 ScaI AGT'ACT Cuts at: 0 925 1434 Size: 925 509 SexAI A'CCwGG_T Cuts at: 0 1070 1434 Size: 1070 364 SmlI C'TyrA_G Cuts at: 0 679 998 1434 Size: 679 319 436 Fragments arranged by size: 679 436 319 StuI AGG'CCT Cuts at: 0 964 1434 Size: 964 470 StyI C'CwwG_G Cuts at: 0 323 1434 Size: 323 1111 TatI w'GTAC_w Cuts at: 0 260 923 1434 Size: 260 663 511 Fragments arranged by size: 663 511 260 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 290 322 1434 Size: 290 32 1112 Fragments arranged by size: 1112 290 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 412 1434 Size: 412 1022 XmnI GAAnn'nnTTC Cuts at: 0 284 1434 Size: 284 1150 Enzymes that do cut: AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApoI BamHI BanI BanII BbsI BciVI BclI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrDI BssSI BstEII BstYI BtgI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HindIII MscI MslI MspA1I NcoI NdeI PasI PflMI PfoI PspOMI PvuII SacI SacII SapI ScaI SexAI SmlI StuI StyI TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI BcgI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaAI BsaBI BsaHI BseYI BsiEI BsiWI BsmI BspEI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI ZraI